Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_594 and Pf6N2E2_595 are separated by 197 nucleotides Pf6N2E2_595 and Pf6N2E2_596 are separated by 37 nucleotides Pf6N2E2_596 and Pf6N2E2_597 are separated by 120 nucleotides
Pf6N2E2_594 - FMN reductase, NADPH-dependent, at 670,473 to 671,012
_594
Pf6N2E2_595 - Arsenical resistance operon repressor, at 671,210 to 671,560
_595
Pf6N2E2_596 - Arsenic efflux pump protein, at 671,598 to 672,881
_596
Pf6N2E2_597 - FIG00953564: hypothetical protein, at 673,002 to 673,967
_597
Position (kb) 671
672
673 Strain fitness (log2 ratio) -2
-1
0
1
2 at 670.598 kb on + strand, within Pf6N2E2_594 at 670.598 kb on + strand, within Pf6N2E2_594 at 670.599 kb on - strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.615 kb on + strand, within Pf6N2E2_594 at 670.632 kb on + strand, within Pf6N2E2_594 at 670.632 kb on + strand, within Pf6N2E2_594 at 670.632 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.692 kb on + strand, within Pf6N2E2_594 at 670.693 kb on - strand, within Pf6N2E2_594 at 670.968 kb on - strand at 670.968 kb on - strand at 671.031 kb on - strand at 671.173 kb on - strand at 671.174 kb on + strand at 671.175 kb on - strand at 671.175 kb on - strand at 671.188 kb on + strand at 671.206 kb on - strand at 671.206 kb on - strand at 671.420 kb on + strand, within Pf6N2E2_595 at 671.420 kb on + strand, within Pf6N2E2_595 at 671.424 kb on - strand, within Pf6N2E2_595 at 671.424 kb on - strand, within Pf6N2E2_595 at 671.633 kb on + strand at 671.633 kb on + strand at 671.633 kb on + strand at 671.753 kb on + strand, within Pf6N2E2_596 at 671.771 kb on + strand, within Pf6N2E2_596 at 671.771 kb on + strand, within Pf6N2E2_596 at 671.772 kb on - strand, within Pf6N2E2_596 at 671.888 kb on + strand, within Pf6N2E2_596 at 671.888 kb on + strand, within Pf6N2E2_596 at 671.888 kb on + strand, within Pf6N2E2_596 at 672.271 kb on - strand, within Pf6N2E2_596 at 672.465 kb on + strand, within Pf6N2E2_596 at 672.465 kb on + strand, within Pf6N2E2_596 at 672.465 kb on + strand, within Pf6N2E2_596 at 672.477 kb on + strand, within Pf6N2E2_596 at 672.507 kb on + strand, within Pf6N2E2_596 at 672.684 kb on + strand, within Pf6N2E2_596 at 672.684 kb on + strand, within Pf6N2E2_596 at 672.684 kb on + strand, within Pf6N2E2_596 at 672.798 kb on + strand at 672.798 kb on + strand at 672.801 kb on + strand at 672.801 kb on + strand at 672.801 kb on + strand at 672.801 kb on + strand at 672.801 kb on + strand at 672.802 kb on - strand at 672.802 kb on - strand at 672.802 kb on - strand at 672.940 kb on - strand at 673.048 kb on - strand at 673.339 kb on + strand, within Pf6N2E2_597 at 673.378 kb on - strand, within Pf6N2E2_597 at 673.536 kb on - strand, within Pf6N2E2_597 at 673.555 kb on + strand, within Pf6N2E2_597 at 673.555 kb on + strand, within Pf6N2E2_597 at 673.556 kb on - strand, within Pf6N2E2_597 at 673.556 kb on - strand, within Pf6N2E2_597 at 673.603 kb on - strand, within Pf6N2E2_597 at 673.718 kb on - strand, within Pf6N2E2_597 at 673.718 kb on - strand, within Pf6N2E2_597
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 670,598 + Pf6N2E2_594 0.23 +1.2 670,598 + Pf6N2E2_594 0.23 +0.9 670,599 - Pf6N2E2_594 0.23 +1.5 670,615 + Pf6N2E2_594 0.26 -1.0 670,615 + Pf6N2E2_594 0.26 +0.9 670,615 + Pf6N2E2_594 0.26 -0.6 670,615 + Pf6N2E2_594 0.26 +0.2 670,632 + Pf6N2E2_594 0.29 +0.6 670,632 + Pf6N2E2_594 0.29 +0.5 670,632 + Pf6N2E2_594 0.29 +0.3 670,692 + Pf6N2E2_594 0.41 +0.3 670,692 + Pf6N2E2_594 0.41 -0.1 670,692 + Pf6N2E2_594 0.41 +0.5 670,692 + Pf6N2E2_594 0.41 +0.6 670,693 - Pf6N2E2_594 0.41 +0.7 670,968 - +0.9 670,968 - -0.7 671,031 - -1.0 671,173 - +0.1 671,174 + +0.2 671,175 - +0.1 671,175 - -0.2 671,188 + -0.9 671,206 - -0.2 671,206 - +0.1 671,420 + Pf6N2E2_595 0.60 -1.1 671,420 + Pf6N2E2_595 0.60 -1.5 671,424 - Pf6N2E2_595 0.61 -1.3 671,424 - Pf6N2E2_595 0.61 -0.6 671,633 + -0.1 671,633 + -0.6 671,633 + -0.3 671,753 + Pf6N2E2_596 0.12 -0.4 671,771 + Pf6N2E2_596 0.13 +0.6 671,771 + Pf6N2E2_596 0.13 -0.1 671,772 - Pf6N2E2_596 0.14 -0.0 671,888 + Pf6N2E2_596 0.23 -0.8 671,888 + Pf6N2E2_596 0.23 -1.2 671,888 + Pf6N2E2_596 0.23 -0.2 672,271 - Pf6N2E2_596 0.52 +0.9 672,465 + Pf6N2E2_596 0.68 +0.6 672,465 + Pf6N2E2_596 0.68 -0.4 672,465 + Pf6N2E2_596 0.68 +0.6 672,477 + Pf6N2E2_596 0.68 +0.9 672,507 + Pf6N2E2_596 0.71 +0.1 672,684 + Pf6N2E2_596 0.85 +0.5 672,684 + Pf6N2E2_596 0.85 -0.6 672,684 + Pf6N2E2_596 0.85 +1.1 672,798 + -0.5 672,798 + +1.8 672,801 + +0.2 672,801 + -0.4 672,801 + +0.5 672,801 + -0.3 672,801 + -0.5 672,802 - +1.0 672,802 - +1.1 672,802 - -0.3 672,940 - -0.7 673,048 - -0.4 673,339 + Pf6N2E2_597 0.35 -0.7 673,378 - Pf6N2E2_597 0.39 -0.3 673,536 - Pf6N2E2_597 0.55 -0.0 673,555 + Pf6N2E2_597 0.57 -0.3 673,555 + Pf6N2E2_597 0.57 -1.9 673,556 - Pf6N2E2_597 0.57 -0.7 673,556 - Pf6N2E2_597 0.57 +0.7 673,603 - Pf6N2E2_597 0.62 -0.6 673,718 - Pf6N2E2_597 0.74 -1.1 673,718 - Pf6N2E2_597 0.74 +0.2
Or see this region's nucleotide sequence