Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_467

Experiment: Fusidic 0.3 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_465 and Pf6N2E2_466 are separated by 41 nucleotidesPf6N2E2_466 and Pf6N2E2_467 are separated by 296 nucleotidesPf6N2E2_467 and Pf6N2E2_468 are separated by 6 nucleotides Pf6N2E2_465 - Probable transmembrane protein, at 530,730 to 531,371 _465 Pf6N2E2_466 - Nicotinamidase (EC 3.5.1.19), at 531,413 to 532,066 _466 Pf6N2E2_467 - Sensory box/GGDEF family protein, at 532,363 to 534,441 _467 Pf6N2E2_468 - DNA alkylation repair enzyme, at 534,448 to 535,551 _468 Position (kb) 532 533 534 535Strain fitness (log2 ratio) -2 -1 0 1 2at 531.401 kb on + strandat 531.401 kb on + strandat 531.401 kb on + strandat 531.401 kb on + strandat 531.401 kb on + strandat 531.404 kb on + strandat 531.404 kb on + strandat 531.405 kb on - strandat 531.405 kb on - strandat 531.434 kb on + strandat 531.435 kb on - strandat 531.435 kb on - strandat 531.615 kb on + strand, within Pf6N2E2_466at 531.615 kb on + strand, within Pf6N2E2_466at 531.616 kb on - strand, within Pf6N2E2_466at 531.696 kb on + strand, within Pf6N2E2_466at 531.697 kb on - strand, within Pf6N2E2_466at 531.697 kb on - strand, within Pf6N2E2_466at 531.792 kb on - strand, within Pf6N2E2_466at 531.792 kb on - strand, within Pf6N2E2_466at 531.792 kb on - strand, within Pf6N2E2_466at 532.014 kb on - strandat 532.014 kb on - strandat 532.100 kb on + strandat 532.101 kb on - strandat 532.101 kb on - strandat 532.126 kb on + strandat 532.127 kb on - strandat 532.127 kb on - strandat 532.200 kb on - strandat 532.244 kb on + strandat 532.244 kb on + strandat 532.297 kb on + strandat 532.297 kb on + strandat 532.297 kb on + strandat 532.297 kb on + strandat 532.297 kb on + strandat 532.298 kb on - strandat 532.302 kb on - strandat 532.302 kb on - strandat 532.349 kb on + strandat 532.349 kb on + strandat 532.378 kb on + strandat 532.378 kb on + strandat 532.379 kb on - strandat 532.429 kb on + strandat 532.429 kb on + strandat 532.429 kb on + strandat 532.429 kb on + strandat 532.430 kb on - strandat 532.430 kb on - strandat 532.430 kb on - strandat 532.589 kb on + strand, within Pf6N2E2_467at 532.589 kb on + strand, within Pf6N2E2_467at 532.590 kb on - strand, within Pf6N2E2_467at 532.590 kb on - strand, within Pf6N2E2_467at 532.590 kb on - strand, within Pf6N2E2_467at 532.590 kb on - strand, within Pf6N2E2_467at 532.729 kb on + strand, within Pf6N2E2_467at 532.729 kb on + strand, within Pf6N2E2_467at 532.730 kb on - strand, within Pf6N2E2_467at 532.730 kb on - strand, within Pf6N2E2_467at 532.772 kb on - strand, within Pf6N2E2_467at 532.930 kb on + strand, within Pf6N2E2_467at 532.930 kb on + strand, within Pf6N2E2_467at 532.930 kb on + strand, within Pf6N2E2_467at 532.931 kb on - strand, within Pf6N2E2_467at 532.931 kb on - strand, within Pf6N2E2_467at 533.716 kb on + strand, within Pf6N2E2_467at 533.717 kb on - strand, within Pf6N2E2_467at 533.719 kb on + strand, within Pf6N2E2_467at 533.905 kb on + strand, within Pf6N2E2_467at 533.905 kb on + strand, within Pf6N2E2_467at 533.905 kb on + strand, within Pf6N2E2_467at 533.906 kb on - strand, within Pf6N2E2_467at 533.906 kb on - strand, within Pf6N2E2_467at 533.906 kb on - strand, within Pf6N2E2_467at 533.919 kb on + strand, within Pf6N2E2_467at 533.919 kb on + strand, within Pf6N2E2_467at 533.920 kb on - strand, within Pf6N2E2_467at 534.160 kb on + strand, within Pf6N2E2_467at 534.160 kb on + strand, within Pf6N2E2_467at 534.160 kb on + strand, within Pf6N2E2_467at 534.161 kb on - strand, within Pf6N2E2_467at 534.651 kb on - strand, within Pf6N2E2_468at 534.971 kb on + strand, within Pf6N2E2_468at 534.971 kb on + strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 534.972 kb on - strand, within Pf6N2E2_468at 535.096 kb on + strand, within Pf6N2E2_468at 535.208 kb on + strand, within Pf6N2E2_468at 535.209 kb on - strand, within Pf6N2E2_468at 535.251 kb on - strand, within Pf6N2E2_468at 535.251 kb on - strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.262 kb on + strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.263 kb on - strand, within Pf6N2E2_468at 535.354 kb on + strand, within Pf6N2E2_468

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml
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531,401 + +0.9
531,401 + -2.0
531,401 + -0.6
531,401 + -0.3
531,401 + +1.1
531,404 + -0.2
531,404 + -0.3
531,405 - +0.1
531,405 - +0.2
531,434 + +0.4
531,435 - +0.2
531,435 - +0.0
531,615 + Pf6N2E2_466 0.31 +1.4
531,615 + Pf6N2E2_466 0.31 +0.0
531,616 - Pf6N2E2_466 0.31 +0.2
531,696 + Pf6N2E2_466 0.43 -0.8
531,697 - Pf6N2E2_466 0.43 +1.1
531,697 - Pf6N2E2_466 0.43 +0.7
531,792 - Pf6N2E2_466 0.58 -1.1
531,792 - Pf6N2E2_466 0.58 +0.8
531,792 - Pf6N2E2_466 0.58 -0.6
532,014 - -0.3
532,014 - -0.2
532,100 + -0.3
532,101 - -0.7
532,101 - -0.8
532,126 + +0.2
532,127 - -0.1
532,127 - -2.3
532,200 - -1.3
532,244 + +0.4
532,244 + +0.7
532,297 + +0.6
532,297 + +0.3
532,297 + +0.7
532,297 + +1.0
532,297 + -0.9
532,298 - -0.9
532,302 - -0.2
532,302 - -0.4
532,349 + +1.9
532,349 + +1.6
532,378 + +1.2
532,378 + -0.1
532,379 - +0.7
532,429 + -1.7
532,429 + +0.7
532,429 + -0.3
532,429 + +0.2
532,430 - -1.4
532,430 - +0.0
532,430 - +0.1
532,589 + Pf6N2E2_467 0.11 -0.0
532,589 + Pf6N2E2_467 0.11 -0.7
532,590 - Pf6N2E2_467 0.11 -1.2
532,590 - Pf6N2E2_467 0.11 -0.7
532,590 - Pf6N2E2_467 0.11 -0.8
532,590 - Pf6N2E2_467 0.11 +1.0
532,729 + Pf6N2E2_467 0.18 -0.2
532,729 + Pf6N2E2_467 0.18 +1.2
532,730 - Pf6N2E2_467 0.18 -0.4
532,730 - Pf6N2E2_467 0.18 -1.4
532,772 - Pf6N2E2_467 0.20 +0.3
532,930 + Pf6N2E2_467 0.27 -0.6
532,930 + Pf6N2E2_467 0.27 +0.3
532,930 + Pf6N2E2_467 0.27 -0.5
532,931 - Pf6N2E2_467 0.27 -0.0
532,931 - Pf6N2E2_467 0.27 -1.2
533,716 + Pf6N2E2_467 0.65 -1.1
533,717 - Pf6N2E2_467 0.65 -0.4
533,719 + Pf6N2E2_467 0.65 -1.1
533,905 + Pf6N2E2_467 0.74 -0.8
533,905 + Pf6N2E2_467 0.74 -0.0
533,905 + Pf6N2E2_467 0.74 -1.1
533,906 - Pf6N2E2_467 0.74 -2.6
533,906 - Pf6N2E2_467 0.74 -0.8
533,906 - Pf6N2E2_467 0.74 +0.2
533,919 + Pf6N2E2_467 0.75 -0.7
533,919 + Pf6N2E2_467 0.75 -0.4
533,920 - Pf6N2E2_467 0.75 -1.4
534,160 + Pf6N2E2_467 0.86 -0.3
534,160 + Pf6N2E2_467 0.86 -1.3
534,160 + Pf6N2E2_467 0.86 -1.8
534,161 - Pf6N2E2_467 0.86 -0.7
534,651 - Pf6N2E2_468 0.18 +0.1
534,971 + Pf6N2E2_468 0.47 -0.2
534,971 + Pf6N2E2_468 0.47 -0.3
534,972 - Pf6N2E2_468 0.47 -0.4
534,972 - Pf6N2E2_468 0.47 -0.4
534,972 - Pf6N2E2_468 0.47 +0.2
534,972 - Pf6N2E2_468 0.47 +0.0
534,972 - Pf6N2E2_468 0.47 +0.9
535,096 + Pf6N2E2_468 0.59 -1.3
535,208 + Pf6N2E2_468 0.69 +0.2
535,209 - Pf6N2E2_468 0.69 -2.3
535,251 - Pf6N2E2_468 0.73 -0.3
535,251 - Pf6N2E2_468 0.73 +0.7
535,262 + Pf6N2E2_468 0.74 +0.3
535,262 + Pf6N2E2_468 0.74 +0.8
535,262 + Pf6N2E2_468 0.74 +0.2
535,262 + Pf6N2E2_468 0.74 -0.2
535,262 + Pf6N2E2_468 0.74 +0.4
535,263 - Pf6N2E2_468 0.74 -0.7
535,263 - Pf6N2E2_468 0.74 +0.5
535,263 - Pf6N2E2_468 0.74 +1.2
535,263 - Pf6N2E2_468 0.74 +0.2
535,354 + Pf6N2E2_468 0.82 +0.2

Or see this region's nucleotide sequence