Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_446 and Pf6N2E2_447 are separated by 174 nucleotides Pf6N2E2_447 and Pf6N2E2_448 are separated by 124 nucleotides Pf6N2E2_448 and Pf6N2E2_449 are separated by 87 nucleotides Pf6N2E2_449 and Pf6N2E2_450 are separated by 194 nucleotides
Pf6N2E2_446 - Glutathione reductase (EC 1.8.1.7), at 508,167 to 509,525
_446
Pf6N2E2_447 - Alkyl hydroperoxide reductase protein C (EC 1.6.4.-), at 509,700 to 510,263
_447
Pf6N2E2_448 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 510,388 to 511,950
_448
Pf6N2E2_449 - Lactoylglutathione lyase (EC 4.4.1.5), at 512,038 to 512,559
_449
Pf6N2E2_450 - FIG00953950: hypothetical protein, at 512,754 to 513,281
_450
Position (kb)
510
511
512 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 509.507 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.540 kb on + strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.541 kb on - strand at 509.580 kb on - strand at 509.583 kb on + strand at 509.584 kb on - strand at 509.656 kb on + strand at 509.656 kb on + strand at 509.656 kb on + strand at 509.706 kb on - strand at 509.706 kb on - strand at 509.706 kb on - strand at 509.724 kb on - strand at 509.814 kb on + strand, within Pf6N2E2_447 at 509.814 kb on + strand, within Pf6N2E2_447 at 509.904 kb on + strand, within Pf6N2E2_447 at 509.905 kb on - strand, within Pf6N2E2_447 at 509.905 kb on - strand, within Pf6N2E2_447 at 509.979 kb on + strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 509.980 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.169 kb on - strand, within Pf6N2E2_447 at 510.261 kb on + strand at 510.261 kb on + strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.262 kb on - strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.298 kb on + strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.299 kb on - strand at 510.387 kb on + strand at 510.388 kb on - strand at 510.388 kb on - strand at 510.405 kb on + strand at 510.405 kb on + strand at 510.424 kb on + strand at 510.504 kb on - strand at 510.538 kb on - strand at 510.538 kb on - strand at 510.538 kb on - strand at 510.538 kb on - strand at 510.754 kb on + strand, within Pf6N2E2_448 at 510.754 kb on + strand, within Pf6N2E2_448 at 510.755 kb on - strand, within Pf6N2E2_448 at 510.755 kb on - strand, within Pf6N2E2_448 at 510.755 kb on - strand, within Pf6N2E2_448 at 510.907 kb on + strand, within Pf6N2E2_448 at 510.908 kb on - strand, within Pf6N2E2_448 at 511.072 kb on + strand, within Pf6N2E2_448 at 511.072 kb on + strand, within Pf6N2E2_448 at 511.072 kb on + strand, within Pf6N2E2_448 at 511.073 kb on - strand, within Pf6N2E2_448 at 511.083 kb on + strand, within Pf6N2E2_448 at 511.222 kb on + strand, within Pf6N2E2_448 at 511.391 kb on - strand, within Pf6N2E2_448 at 511.411 kb on + strand, within Pf6N2E2_448 at 511.473 kb on + strand, within Pf6N2E2_448 at 511.473 kb on + strand, within Pf6N2E2_448 at 511.608 kb on + strand, within Pf6N2E2_448 at 511.609 kb on - strand, within Pf6N2E2_448 at 511.861 kb on + strand at 511.861 kb on + strand at 511.862 kb on - strand at 511.948 kb on + strand at 512.039 kb on + strand at 512.040 kb on - strand at 512.072 kb on + strand at 512.073 kb on - strand at 512.237 kb on + strand, within Pf6N2E2_449 at 512.370 kb on - strand, within Pf6N2E2_449 at 512.370 kb on - strand, within Pf6N2E2_449 at 512.370 kb on - strand, within Pf6N2E2_449 at 512.435 kb on + strand, within Pf6N2E2_449 at 512.573 kb on - strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.574 kb on + strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.575 kb on - strand at 512.658 kb on + strand at 512.659 kb on - strand at 512.659 kb on - strand at 512.659 kb on - strand at 512.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 509,507 + -0.9 509,540 + -1.1 509,540 + +0.7 509,540 + +0.0 509,540 + +0.1 509,540 + -0.4 509,540 + -0.2 509,540 + -1.1 509,540 + +0.0 509,541 - -1.1 509,541 - -0.6 509,541 - +0.1 509,541 - -0.3 509,580 - -1.5 509,583 + +1.2 509,584 - -0.3 509,656 + -0.3 509,656 + +0.3 509,656 + +0.8 509,706 - -2.1 509,706 - -0.8 509,706 - +0.8 509,724 - -0.2 509,814 + Pf6N2E2_447 0.20 -1.4 509,814 + Pf6N2E2_447 0.20 -2.3 509,904 + Pf6N2E2_447 0.36 -0.9 509,905 - Pf6N2E2_447 0.36 -2.2 509,905 - Pf6N2E2_447 0.36 -2.4 509,979 + Pf6N2E2_447 0.49 +2.9 509,980 - Pf6N2E2_447 0.50 -1.8 509,980 - Pf6N2E2_447 0.50 +0.3 509,980 - Pf6N2E2_447 0.50 -1.4 510,169 - Pf6N2E2_447 0.83 -0.7 510,169 - Pf6N2E2_447 0.83 +0.5 510,169 - Pf6N2E2_447 0.83 -0.8 510,261 + +0.5 510,261 + -0.3 510,262 - -0.1 510,262 - +1.2 510,262 - -0.4 510,262 - -0.1 510,262 - -0.6 510,262 - -1.2 510,262 - -0.3 510,298 + -0.3 510,298 + -2.1 510,298 + -0.0 510,298 + +0.6 510,299 - -0.2 510,299 - +0.7 510,299 - +1.9 510,299 - +0.3 510,299 - +0.2 510,299 - +0.8 510,299 - +0.7 510,387 + -0.5 510,388 - -0.5 510,388 - -1.8 510,405 + +0.7 510,405 + -0.4 510,424 + +0.7 510,504 - +1.6 510,538 - +1.5 510,538 - +0.5 510,538 - +0.4 510,538 - +0.6 510,754 + Pf6N2E2_448 0.23 +0.3 510,754 + Pf6N2E2_448 0.23 -0.7 510,755 - Pf6N2E2_448 0.23 +0.3 510,755 - Pf6N2E2_448 0.23 -0.3 510,755 - Pf6N2E2_448 0.23 -1.0 510,907 + Pf6N2E2_448 0.33 -0.1 510,908 - Pf6N2E2_448 0.33 +0.7 511,072 + Pf6N2E2_448 0.44 +0.2 511,072 + Pf6N2E2_448 0.44 -0.9 511,072 + Pf6N2E2_448 0.44 -0.1 511,073 - Pf6N2E2_448 0.44 -1.4 511,083 + Pf6N2E2_448 0.44 +0.8 511,222 + Pf6N2E2_448 0.53 -0.6 511,391 - Pf6N2E2_448 0.64 -2.7 511,411 + Pf6N2E2_448 0.65 -1.4 511,473 + Pf6N2E2_448 0.69 -0.5 511,473 + Pf6N2E2_448 0.69 -1.5 511,608 + Pf6N2E2_448 0.78 -0.7 511,609 - Pf6N2E2_448 0.78 -0.7 511,861 + -0.6 511,861 + -0.5 511,862 - -1.6 511,948 + -1.9 512,039 + -0.5 512,040 - -1.2 512,072 + -1.0 512,073 - +0.5 512,237 + Pf6N2E2_449 0.38 +0.6 512,370 - Pf6N2E2_449 0.64 -0.3 512,370 - Pf6N2E2_449 0.64 -0.4 512,370 - Pf6N2E2_449 0.64 -0.5 512,435 + Pf6N2E2_449 0.76 +0.9 512,573 - -1.5 512,574 + +0.5 512,574 + +0.4 512,574 + +0.1 512,574 + +0.9 512,574 + +0.6 512,574 + +1.0 512,574 + -1.3 512,574 + -0.0 512,574 + -0.4 512,575 - -1.6 512,575 - +0.4 512,575 - -1.2 512,575 - +0.3 512,575 - +0.0 512,658 + -0.3 512,659 - -1.2 512,659 - +0.3 512,659 - -0.4 512,659 - +0.7
Or see this region's nucleotide sequence