Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4120 and Pf6N2E2_4121 are separated by 24 nucleotides Pf6N2E2_4121 and Pf6N2E2_4122 overlap by 4 nucleotides
Pf6N2E2_4120 - Trk system potassium uptake protein TrkA, at 4,798,826 to 4,800,199
_4120
Pf6N2E2_4121 - Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-), at 4,800,224 to 4,801,534
_4121
Pf6N2E2_4122 - Methionyl-tRNA formyltransferase (EC 2.1.2.9), at 4,801,531 to 4,802,490
_4122
Position (kb)
4800
4801
4802 Strain fitness (log2 ratio)
-2
-1
0
1 at 4799.398 kb on - strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.508 kb on + strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.509 kb on - strand, within Pf6N2E2_4120 at 4799.552 kb on - strand, within Pf6N2E2_4120 at 4799.719 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.722 kb on - strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.806 kb on + strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.807 kb on - strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.808 kb on + strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4799.809 kb on - strand, within Pf6N2E2_4120 at 4800.261 kb on + strand at 4800.262 kb on - strand at 4800.262 kb on - strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.264 kb on + strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.265 kb on - strand at 4800.436 kb on + strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.437 kb on - strand, within Pf6N2E2_4121 at 4800.933 kb on + strand, within Pf6N2E2_4121 at 4800.934 kb on - strand, within Pf6N2E2_4121 at 4800.982 kb on - strand, within Pf6N2E2_4121 at 4802.495 kb on - strand at 4802.495 kb on - strand at 4802.495 kb on - strand at 4802.506 kb on - strand at 4802.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 4,799,398 - Pf6N2E2_4120 0.42 -1.5 4,799,508 + Pf6N2E2_4120 0.50 -1.3 4,799,508 + Pf6N2E2_4120 0.50 -0.4 4,799,508 + Pf6N2E2_4120 0.50 +0.3 4,799,508 + Pf6N2E2_4120 0.50 -0.4 4,799,508 + Pf6N2E2_4120 0.50 -0.5 4,799,508 + Pf6N2E2_4120 0.50 -2.5 4,799,509 - Pf6N2E2_4120 0.50 +0.8 4,799,509 - Pf6N2E2_4120 0.50 +0.5 4,799,509 - Pf6N2E2_4120 0.50 +0.3 4,799,509 - Pf6N2E2_4120 0.50 +0.7 4,799,509 - Pf6N2E2_4120 0.50 +0.7 4,799,509 - Pf6N2E2_4120 0.50 -0.6 4,799,509 - Pf6N2E2_4120 0.50 +0.4 4,799,509 - Pf6N2E2_4120 0.50 -0.6 4,799,509 - Pf6N2E2_4120 0.50 +0.4 4,799,509 - Pf6N2E2_4120 0.50 +0.1 4,799,552 - Pf6N2E2_4120 0.53 -0.3 4,799,719 - Pf6N2E2_4120 0.65 +0.1 4,799,722 - Pf6N2E2_4120 0.65 +0.3 4,799,722 - Pf6N2E2_4120 0.65 -1.3 4,799,722 - Pf6N2E2_4120 0.65 +0.1 4,799,806 + Pf6N2E2_4120 0.71 +0.9 4,799,806 + Pf6N2E2_4120 0.71 -0.9 4,799,806 + Pf6N2E2_4120 0.71 -0.2 4,799,806 + Pf6N2E2_4120 0.71 -1.3 4,799,807 - Pf6N2E2_4120 0.71 -0.5 4,799,807 - Pf6N2E2_4120 0.71 -0.2 4,799,807 - Pf6N2E2_4120 0.71 -0.8 4,799,807 - Pf6N2E2_4120 0.71 +0.5 4,799,807 - Pf6N2E2_4120 0.71 -0.3 4,799,808 + Pf6N2E2_4120 0.71 -0.0 4,799,808 + Pf6N2E2_4120 0.71 -0.0 4,799,808 + Pf6N2E2_4120 0.71 -1.1 4,799,808 + Pf6N2E2_4120 0.71 -0.8 4,799,808 + Pf6N2E2_4120 0.71 +0.2 4,799,808 + Pf6N2E2_4120 0.71 +0.7 4,799,808 + Pf6N2E2_4120 0.71 +0.6 4,799,808 + Pf6N2E2_4120 0.71 +0.7 4,799,808 + Pf6N2E2_4120 0.71 +0.2 4,799,808 + Pf6N2E2_4120 0.71 -1.5 4,799,808 + Pf6N2E2_4120 0.71 -2.3 4,799,808 + Pf6N2E2_4120 0.71 +0.5 4,799,808 + Pf6N2E2_4120 0.71 +1.2 4,799,808 + Pf6N2E2_4120 0.71 +0.5 4,799,808 + Pf6N2E2_4120 0.71 +0.4 4,799,808 + Pf6N2E2_4120 0.71 +0.1 4,799,808 + Pf6N2E2_4120 0.71 -0.6 4,799,808 + Pf6N2E2_4120 0.71 -2.3 4,799,808 + Pf6N2E2_4120 0.71 -0.4 4,799,809 - Pf6N2E2_4120 0.72 -0.8 4,799,809 - Pf6N2E2_4120 0.72 +0.8 4,799,809 - Pf6N2E2_4120 0.72 +0.1 4,799,809 - Pf6N2E2_4120 0.72 +0.8 4,799,809 - Pf6N2E2_4120 0.72 -0.0 4,799,809 - Pf6N2E2_4120 0.72 -0.6 4,799,809 - Pf6N2E2_4120 0.72 -0.5 4,799,809 - Pf6N2E2_4120 0.72 +0.5 4,799,809 - Pf6N2E2_4120 0.72 +0.3 4,799,809 - Pf6N2E2_4120 0.72 -2.1 4,799,809 - Pf6N2E2_4120 0.72 +0.2 4,799,809 - Pf6N2E2_4120 0.72 -2.2 4,799,809 - Pf6N2E2_4120 0.72 +0.3 4,799,809 - Pf6N2E2_4120 0.72 -0.3 4,799,809 - Pf6N2E2_4120 0.72 -1.9 4,799,809 - Pf6N2E2_4120 0.72 +0.2 4,799,809 - Pf6N2E2_4120 0.72 +0.6 4,799,809 - Pf6N2E2_4120 0.72 -0.2 4,800,261 + -0.1 4,800,262 - -0.8 4,800,262 - -0.1 4,800,264 + +0.9 4,800,264 + +0.3 4,800,264 + -0.2 4,800,264 + +0.2 4,800,265 - -0.6 4,800,265 - -0.9 4,800,265 - -1.8 4,800,265 - +1.3 4,800,436 + Pf6N2E2_4121 0.16 -2.5 4,800,437 - Pf6N2E2_4121 0.16 -0.3 4,800,437 - Pf6N2E2_4121 0.16 -1.7 4,800,437 - Pf6N2E2_4121 0.16 +0.3 4,800,437 - Pf6N2E2_4121 0.16 +0.1 4,800,933 + Pf6N2E2_4121 0.54 -0.6 4,800,934 - Pf6N2E2_4121 0.54 -0.5 4,800,982 - Pf6N2E2_4121 0.58 -1.0 4,802,495 - +0.5 4,802,495 - -1.2 4,802,495 - +0.1 4,802,506 - +0.9 4,802,506 - -0.3
Or see this region's nucleotide sequence