Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3745 and Pf6N2E2_3746 are separated by 17 nucleotides Pf6N2E2_3746 and Pf6N2E2_3747 overlap by 4 nucleotides Pf6N2E2_3747 and Pf6N2E2_3748 overlap by 4 nucleotides
Pf6N2E2_3745 - General secretion pathway protein E, at 4,359,369 to 4,360,820
_3745
Pf6N2E2_3746 - General secretion pathway protein D, at 4,360,838 to 4,362,772
_3746
Pf6N2E2_3747 - hypothetical protein, at 4,362,769 to 4,363,293
_3747
Pf6N2E2_3748 - Cytochrome B561, at 4,363,290 to 4,363,835
_3748
Position (kb)
4360
4361
4362
4363 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4359.874 kb on - strand, within Pf6N2E2_3745 at 4359.874 kb on - strand, within Pf6N2E2_3745 at 4359.874 kb on - strand, within Pf6N2E2_3745 at 4359.988 kb on + strand, within Pf6N2E2_3745 at 4359.989 kb on - strand, within Pf6N2E2_3745 at 4360.054 kb on + strand, within Pf6N2E2_3745 at 4360.055 kb on - strand, within Pf6N2E2_3745 at 4360.055 kb on - strand, within Pf6N2E2_3745 at 4360.251 kb on + strand, within Pf6N2E2_3745 at 4360.251 kb on + strand, within Pf6N2E2_3745 at 4360.429 kb on + strand, within Pf6N2E2_3745 at 4360.429 kb on + strand, within Pf6N2E2_3745 at 4360.429 kb on + strand, within Pf6N2E2_3745 at 4360.429 kb on + strand, within Pf6N2E2_3745 at 4360.430 kb on - strand, within Pf6N2E2_3745 at 4360.430 kb on - strand, within Pf6N2E2_3745 at 4360.430 kb on - strand, within Pf6N2E2_3745 at 4360.430 kb on - strand, within Pf6N2E2_3745 at 4360.430 kb on - strand, within Pf6N2E2_3745 at 4360.637 kb on - strand, within Pf6N2E2_3745 at 4360.978 kb on - strand at 4361.104 kb on + strand, within Pf6N2E2_3746 at 4361.319 kb on + strand, within Pf6N2E2_3746 at 4361.319 kb on + strand, within Pf6N2E2_3746 at 4361.319 kb on + strand, within Pf6N2E2_3746 at 4361.319 kb on + strand, within Pf6N2E2_3746 at 4361.320 kb on - strand, within Pf6N2E2_3746 at 4361.320 kb on - strand, within Pf6N2E2_3746 at 4361.404 kb on - strand, within Pf6N2E2_3746 at 4361.503 kb on + strand, within Pf6N2E2_3746 at 4361.578 kb on + strand, within Pf6N2E2_3746 at 4361.587 kb on + strand, within Pf6N2E2_3746 at 4361.588 kb on - strand, within Pf6N2E2_3746 at 4361.588 kb on - strand, within Pf6N2E2_3746 at 4362.292 kb on - strand, within Pf6N2E2_3746 at 4362.292 kb on - strand, within Pf6N2E2_3746 at 4362.292 kb on - strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.501 kb on + strand, within Pf6N2E2_3746 at 4362.502 kb on - strand, within Pf6N2E2_3746 at 4362.502 kb on - strand, within Pf6N2E2_3746 at 4362.502 kb on - strand, within Pf6N2E2_3746 at 4362.735 kb on - strand at 4362.735 kb on - strand at 4362.796 kb on + strand at 4362.796 kb on + strand at 4362.800 kb on + strand at 4362.800 kb on + strand at 4362.800 kb on + strand at 4362.800 kb on + strand at 4362.801 kb on - strand at 4362.801 kb on - strand at 4362.977 kb on + strand, within Pf6N2E2_3747 at 4362.978 kb on - strand, within Pf6N2E2_3747 at 4363.053 kb on + strand, within Pf6N2E2_3747 at 4363.053 kb on + strand, within Pf6N2E2_3747 at 4363.053 kb on + strand, within Pf6N2E2_3747 at 4363.054 kb on - strand, within Pf6N2E2_3747 at 4363.054 kb on - strand, within Pf6N2E2_3747 at 4363.230 kb on - strand, within Pf6N2E2_3747 at 4363.347 kb on + strand, within Pf6N2E2_3748 at 4363.348 kb on - strand, within Pf6N2E2_3748 at 4363.433 kb on + strand, within Pf6N2E2_3748 at 4363.564 kb on + strand, within Pf6N2E2_3748 at 4363.564 kb on + strand, within Pf6N2E2_3748 at 4363.564 kb on + strand, within Pf6N2E2_3748 at 4363.565 kb on - strand, within Pf6N2E2_3748 at 4363.565 kb on - strand, within Pf6N2E2_3748 at 4363.750 kb on + strand, within Pf6N2E2_3748 at 4363.751 kb on - strand, within Pf6N2E2_3748 at 4363.751 kb on - strand, within Pf6N2E2_3748
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 4,359,874 - Pf6N2E2_3745 0.35 -0.3 4,359,874 - Pf6N2E2_3745 0.35 -0.9 4,359,874 - Pf6N2E2_3745 0.35 -0.1 4,359,988 + Pf6N2E2_3745 0.43 +1.2 4,359,989 - Pf6N2E2_3745 0.43 +2.1 4,360,054 + Pf6N2E2_3745 0.47 -0.9 4,360,055 - Pf6N2E2_3745 0.47 -0.0 4,360,055 - Pf6N2E2_3745 0.47 +0.3 4,360,251 + Pf6N2E2_3745 0.61 +0.2 4,360,251 + Pf6N2E2_3745 0.61 +0.6 4,360,429 + Pf6N2E2_3745 0.73 +0.3 4,360,429 + Pf6N2E2_3745 0.73 -1.0 4,360,429 + Pf6N2E2_3745 0.73 +0.0 4,360,429 + Pf6N2E2_3745 0.73 +0.8 4,360,430 - Pf6N2E2_3745 0.73 +1.4 4,360,430 - Pf6N2E2_3745 0.73 +1.0 4,360,430 - Pf6N2E2_3745 0.73 -0.1 4,360,430 - Pf6N2E2_3745 0.73 -0.6 4,360,430 - Pf6N2E2_3745 0.73 -0.4 4,360,637 - Pf6N2E2_3745 0.87 +0.4 4,360,978 - -1.4 4,361,104 + Pf6N2E2_3746 0.14 -0.5 4,361,319 + Pf6N2E2_3746 0.25 -0.5 4,361,319 + Pf6N2E2_3746 0.25 +0.3 4,361,319 + Pf6N2E2_3746 0.25 +0.2 4,361,319 + Pf6N2E2_3746 0.25 -0.3 4,361,320 - Pf6N2E2_3746 0.25 -0.3 4,361,320 - Pf6N2E2_3746 0.25 -0.3 4,361,404 - Pf6N2E2_3746 0.29 +0.1 4,361,503 + Pf6N2E2_3746 0.34 +0.1 4,361,578 + Pf6N2E2_3746 0.38 -1.4 4,361,587 + Pf6N2E2_3746 0.39 -0.3 4,361,588 - Pf6N2E2_3746 0.39 -0.0 4,361,588 - Pf6N2E2_3746 0.39 +0.4 4,362,292 - Pf6N2E2_3746 0.75 +0.4 4,362,292 - Pf6N2E2_3746 0.75 -0.8 4,362,292 - Pf6N2E2_3746 0.75 +0.1 4,362,501 + Pf6N2E2_3746 0.86 -0.4 4,362,501 + Pf6N2E2_3746 0.86 -0.9 4,362,501 + Pf6N2E2_3746 0.86 -0.8 4,362,501 + Pf6N2E2_3746 0.86 +0.5 4,362,502 - Pf6N2E2_3746 0.86 +0.3 4,362,502 - Pf6N2E2_3746 0.86 -0.6 4,362,502 - Pf6N2E2_3746 0.86 -0.8 4,362,735 - +0.4 4,362,735 - +0.4 4,362,796 + -0.3 4,362,796 + -0.3 4,362,800 + +0.3 4,362,800 + -0.1 4,362,800 + -1.2 4,362,800 + +0.1 4,362,801 - +0.8 4,362,801 - +0.5 4,362,977 + Pf6N2E2_3747 0.40 +0.0 4,362,978 - Pf6N2E2_3747 0.40 -0.5 4,363,053 + Pf6N2E2_3747 0.54 +0.1 4,363,053 + Pf6N2E2_3747 0.54 -0.1 4,363,053 + Pf6N2E2_3747 0.54 -1.1 4,363,054 - Pf6N2E2_3747 0.54 +0.5 4,363,054 - Pf6N2E2_3747 0.54 +0.7 4,363,230 - Pf6N2E2_3747 0.88 +0.5 4,363,347 + Pf6N2E2_3748 0.10 -0.2 4,363,348 - Pf6N2E2_3748 0.11 -1.2 4,363,433 + Pf6N2E2_3748 0.26 -2.0 4,363,564 + Pf6N2E2_3748 0.50 -0.8 4,363,564 + Pf6N2E2_3748 0.50 -1.0 4,363,564 + Pf6N2E2_3748 0.50 -0.2 4,363,565 - Pf6N2E2_3748 0.50 -1.3 4,363,565 - Pf6N2E2_3748 0.50 -0.4 4,363,750 + Pf6N2E2_3748 0.84 -1.0 4,363,751 - Pf6N2E2_3748 0.84 -0.7 4,363,751 - Pf6N2E2_3748 0.84 -0.1
Or see this region's nucleotide sequence