Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_346 and Pf6N2E2_347 are separated by 120 nucleotides Pf6N2E2_347 and Pf6N2E2_348 are separated by 132 nucleotides Pf6N2E2_348 and Pf6N2E2_349 overlap by 4 nucleotides Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides
Pf6N2E2_346 - 3-demethylubiquinone-9 3-methyltransferase, at 412,980 to 413,459
_346
Pf6N2E2_347 - Endoribonuclease L-PSP, at 413,580 to 413,981
_347
Pf6N2E2_348 - Transcriptional regulator, LysR family, at 414,114 to 415,043
_348
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Position (kb) 414
415
416 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 413.219 kb on + strand, within Pf6N2E2_346 at 413.636 kb on - strand, within Pf6N2E2_347 at 413.637 kb on + strand, within Pf6N2E2_347 at 413.637 kb on + strand, within Pf6N2E2_347 at 413.637 kb on + strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.638 kb on - strand, within Pf6N2E2_347 at 413.697 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.935 kb on + strand, within Pf6N2E2_347 at 413.936 kb on - strand, within Pf6N2E2_347 at 413.936 kb on - strand, within Pf6N2E2_347 at 413.985 kb on + strand at 413.986 kb on - strand at 413.986 kb on - strand at 413.986 kb on - strand at 414.026 kb on + strand at 414.027 kb on - strand at 414.306 kb on + strand, within Pf6N2E2_348 at 414.306 kb on + strand, within Pf6N2E2_348 at 414.456 kb on + strand, within Pf6N2E2_348 at 414.457 kb on - strand, within Pf6N2E2_348 at 414.726 kb on + strand, within Pf6N2E2_348 at 414.727 kb on - strand, within Pf6N2E2_348 at 414.801 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.849 kb on + strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.850 kb on - strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 414.927 kb on + strand, within Pf6N2E2_348 at 415.062 kb on + strand at 415.062 kb on + strand at 415.063 kb on - strand at 415.063 kb on - strand at 415.073 kb on + strand at 415.073 kb on + strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.074 kb on - strand at 415.082 kb on + strand at 415.083 kb on - strand at 415.083 kb on - strand at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 413,219 + Pf6N2E2_346 0.50 +1.2 413,636 - Pf6N2E2_347 0.14 -2.0 413,637 + Pf6N2E2_347 0.14 -0.8 413,637 + Pf6N2E2_347 0.14 -0.4 413,637 + Pf6N2E2_347 0.14 -1.1 413,638 - Pf6N2E2_347 0.14 -0.4 413,638 - Pf6N2E2_347 0.14 -2.2 413,638 - Pf6N2E2_347 0.14 +0.7 413,638 - Pf6N2E2_347 0.14 -0.9 413,697 + Pf6N2E2_347 0.29 -1.7 413,935 + Pf6N2E2_347 0.88 +0.5 413,935 + Pf6N2E2_347 0.88 +0.5 413,935 + Pf6N2E2_347 0.88 +1.1 413,935 + Pf6N2E2_347 0.88 +0.7 413,935 + Pf6N2E2_347 0.88 +0.2 413,936 - Pf6N2E2_347 0.89 -0.3 413,936 - Pf6N2E2_347 0.89 -0.1 413,985 + -0.0 413,986 - +0.7 413,986 - +1.0 413,986 - +0.5 414,026 + +0.8 414,027 - -0.2 414,306 + Pf6N2E2_348 0.21 -1.4 414,306 + Pf6N2E2_348 0.21 -0.5 414,456 + Pf6N2E2_348 0.37 -0.5 414,457 - Pf6N2E2_348 0.37 +0.2 414,726 + Pf6N2E2_348 0.66 -1.3 414,727 - Pf6N2E2_348 0.66 +1.0 414,801 + Pf6N2E2_348 0.74 -0.5 414,849 + Pf6N2E2_348 0.79 -0.0 414,849 + Pf6N2E2_348 0.79 +0.6 414,849 + Pf6N2E2_348 0.79 -1.0 414,849 + Pf6N2E2_348 0.79 +0.2 414,849 + Pf6N2E2_348 0.79 -0.4 414,850 - Pf6N2E2_348 0.79 -0.6 414,850 - Pf6N2E2_348 0.79 -1.7 414,850 - Pf6N2E2_348 0.79 +3.0 414,850 - Pf6N2E2_348 0.79 -0.3 414,927 + Pf6N2E2_348 0.87 -0.5 414,927 + Pf6N2E2_348 0.87 +0.3 415,062 + -0.2 415,062 + -0.5 415,063 - -0.5 415,063 - -0.7 415,073 + -0.3 415,073 + -0.0 415,074 - -1.8 415,074 - +0.3 415,074 - -0.8 415,082 + +1.8 415,083 - -2.8 415,083 - -0.5 415,347 + Pf6N2E2_349 0.42 -0.1 415,515 + Pf6N2E2_349 0.65 -0.6 415,516 - Pf6N2E2_349 0.65 +0.9 415,542 - Pf6N2E2_349 0.68 -0.6 415,660 + Pf6N2E2_349 0.84 +0.2 415,660 + Pf6N2E2_349 0.84 -1.3 415,772 + +0.1 415,773 - +0.2 415,806 + +1.9 415,807 - +0.1 415,807 - -1.6 415,807 - -0.5 415,807 - +0.7 415,865 + +1.6
Or see this region's nucleotide sequence