Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2839 and Pf6N2E2_2840 are separated by 577 nucleotides Pf6N2E2_2840 and Pf6N2E2_2841 are separated by 404 nucleotides
Pf6N2E2_2839 - Pca regulon regulatory protein PcaR, at 3,328,869 to 3,329,711
_2839
Pf6N2E2_2840 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,330,289 to 3,330,489
_2840
Pf6N2E2_2841 - Low-affinity inorganic phosphate transporter, at 3,330,894 to 3,332,330
_2841
Position (kb)
3330
3331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3329.291 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.293 kb on + strand, within Pf6N2E2_2839 at 3329.294 kb on - strand, within Pf6N2E2_2839 at 3329.294 kb on - strand, within Pf6N2E2_2839 at 3329.294 kb on - strand, within Pf6N2E2_2839 at 3329.294 kb on - strand, within Pf6N2E2_2839 at 3329.294 kb on - strand, within Pf6N2E2_2839 at 3329.294 kb on - strand, within Pf6N2E2_2839 at 3329.300 kb on + strand, within Pf6N2E2_2839 at 3329.404 kb on + strand, within Pf6N2E2_2839 at 3329.404 kb on + strand, within Pf6N2E2_2839 at 3329.404 kb on + strand, within Pf6N2E2_2839 at 3329.404 kb on + strand, within Pf6N2E2_2839 at 3329.404 kb on + strand, within Pf6N2E2_2839 at 3329.405 kb on - strand, within Pf6N2E2_2839 at 3329.405 kb on - strand, within Pf6N2E2_2839 at 3329.405 kb on - strand, within Pf6N2E2_2839 at 3329.405 kb on - strand, within Pf6N2E2_2839 at 3329.405 kb on - strand, within Pf6N2E2_2839 at 3329.444 kb on - strand, within Pf6N2E2_2839 at 3329.444 kb on - strand, within Pf6N2E2_2839 at 3329.486 kb on + strand, within Pf6N2E2_2839 at 3329.486 kb on + strand, within Pf6N2E2_2839 at 3329.486 kb on + strand, within Pf6N2E2_2839 at 3329.486 kb on + strand, within Pf6N2E2_2839 at 3329.487 kb on - strand, within Pf6N2E2_2839 at 3329.487 kb on - strand, within Pf6N2E2_2839 at 3329.740 kb on - strand at 3329.742 kb on + strand at 3329.742 kb on + strand at 3329.742 kb on + strand at 3329.742 kb on + strand at 3329.742 kb on + strand at 3329.742 kb on + strand at 3329.743 kb on - strand at 3329.743 kb on - strand at 3329.743 kb on - strand at 3329.743 kb on - strand at 3329.743 kb on - strand at 3329.833 kb on + strand at 3329.957 kb on - strand at 3329.998 kb on + strand at 3329.998 kb on + strand at 3329.998 kb on + strand at 3329.998 kb on + strand at 3329.999 kb on - strand at 3329.999 kb on - strand at 3329.999 kb on - strand at 3330.032 kb on + strand at 3330.052 kb on + strand at 3330.053 kb on - strand at 3330.068 kb on + strand at 3330.069 kb on - strand at 3330.069 kb on - strand at 3330.070 kb on + strand at 3330.070 kb on + strand at 3330.071 kb on - strand at 3330.127 kb on + strand at 3330.127 kb on + strand at 3330.127 kb on + strand at 3330.127 kb on + strand at 3330.131 kb on - strand at 3330.153 kb on + strand at 3330.154 kb on - strand at 3330.170 kb on - strand at 3330.198 kb on - strand at 3330.198 kb on - strand at 3330.198 kb on - strand at 3330.239 kb on + strand at 3330.239 kb on + strand at 3330.240 kb on - strand at 3330.240 kb on - strand at 3330.274 kb on + strand at 3330.274 kb on + strand at 3330.274 kb on + strand at 3330.275 kb on - strand at 3330.275 kb on - strand at 3330.278 kb on + strand at 3330.279 kb on - strand at 3330.279 kb on - strand at 3330.282 kb on + strand at 3330.282 kb on + strand at 3330.282 kb on + strand at 3330.282 kb on + strand at 3330.338 kb on - strand, within Pf6N2E2_2840 at 3330.338 kb on - strand, within Pf6N2E2_2840 at 3330.338 kb on - strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.397 kb on - strand, within Pf6N2E2_2840 at 3330.409 kb on - strand, within Pf6N2E2_2840 at 3330.631 kb on - strand at 3330.657 kb on + strand at 3330.657 kb on + strand at 3330.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 3,329,291 + Pf6N2E2_2839 0.50 -0.4 3,329,293 + Pf6N2E2_2839 0.50 -0.3 3,329,293 + Pf6N2E2_2839 0.50 -0.0 3,329,293 + Pf6N2E2_2839 0.50 -0.5 3,329,293 + Pf6N2E2_2839 0.50 -0.6 3,329,293 + Pf6N2E2_2839 0.50 +0.7 3,329,293 + Pf6N2E2_2839 0.50 -0.2 3,329,293 + Pf6N2E2_2839 0.50 +0.8 3,329,294 - Pf6N2E2_2839 0.50 -0.6 3,329,294 - Pf6N2E2_2839 0.50 -0.5 3,329,294 - Pf6N2E2_2839 0.50 -0.5 3,329,294 - Pf6N2E2_2839 0.50 -0.8 3,329,294 - Pf6N2E2_2839 0.50 -2.3 3,329,294 - Pf6N2E2_2839 0.50 -0.3 3,329,300 + Pf6N2E2_2839 0.51 +0.6 3,329,404 + Pf6N2E2_2839 0.63 +0.7 3,329,404 + Pf6N2E2_2839 0.63 +0.1 3,329,404 + Pf6N2E2_2839 0.63 -0.2 3,329,404 + Pf6N2E2_2839 0.63 -0.2 3,329,404 + Pf6N2E2_2839 0.63 -0.9 3,329,405 - Pf6N2E2_2839 0.64 +0.3 3,329,405 - Pf6N2E2_2839 0.64 -0.1 3,329,405 - Pf6N2E2_2839 0.64 -0.3 3,329,405 - Pf6N2E2_2839 0.64 +0.0 3,329,405 - Pf6N2E2_2839 0.64 -0.1 3,329,444 - Pf6N2E2_2839 0.68 -1.4 3,329,444 - Pf6N2E2_2839 0.68 +0.4 3,329,486 + Pf6N2E2_2839 0.73 -0.4 3,329,486 + Pf6N2E2_2839 0.73 +0.7 3,329,486 + Pf6N2E2_2839 0.73 -0.6 3,329,486 + Pf6N2E2_2839 0.73 -0.4 3,329,487 - Pf6N2E2_2839 0.73 -1.1 3,329,487 - Pf6N2E2_2839 0.73 -0.6 3,329,740 - -0.6 3,329,742 + +0.1 3,329,742 + -0.1 3,329,742 + +0.2 3,329,742 + -1.0 3,329,742 + +0.2 3,329,742 + -0.8 3,329,743 - -0.6 3,329,743 - +0.0 3,329,743 - -0.3 3,329,743 - -1.8 3,329,743 - +0.1 3,329,833 + +0.6 3,329,957 - -0.3 3,329,998 + +0.6 3,329,998 + -0.5 3,329,998 + -0.3 3,329,998 + -1.0 3,329,999 - -0.8 3,329,999 - -0.8 3,329,999 - +0.6 3,330,032 + +0.5 3,330,052 + +1.5 3,330,053 - -0.4 3,330,068 + -0.8 3,330,069 - -1.2 3,330,069 - +1.6 3,330,070 + -0.4 3,330,070 + +0.5 3,330,071 - +1.1 3,330,127 + -0.1 3,330,127 + +0.3 3,330,127 + +0.1 3,330,127 + -0.8 3,330,131 - +0.1 3,330,153 + +0.6 3,330,154 - +0.2 3,330,170 - -0.1 3,330,198 - -0.2 3,330,198 - -0.7 3,330,198 - +0.8 3,330,239 + +0.1 3,330,239 + -0.8 3,330,240 - +0.5 3,330,240 - -1.2 3,330,274 + -0.6 3,330,274 + +0.6 3,330,274 + +0.1 3,330,275 - -0.4 3,330,275 - -0.5 3,330,278 + -2.0 3,330,279 - +0.1 3,330,279 - -1.3 3,330,282 + -1.0 3,330,282 + -0.1 3,330,282 + -0.3 3,330,282 + -0.5 3,330,338 - Pf6N2E2_2840 0.24 -0.9 3,330,338 - Pf6N2E2_2840 0.24 -0.6 3,330,338 - Pf6N2E2_2840 0.24 -0.2 3,330,370 + Pf6N2E2_2840 0.40 -0.9 3,330,370 + Pf6N2E2_2840 0.40 -0.6 3,330,370 + Pf6N2E2_2840 0.40 +0.1 3,330,370 + Pf6N2E2_2840 0.40 -1.7 3,330,371 - Pf6N2E2_2840 0.41 -1.5 3,330,371 - Pf6N2E2_2840 0.41 -1.9 3,330,371 - Pf6N2E2_2840 0.41 -0.9 3,330,371 - Pf6N2E2_2840 0.41 -0.9 3,330,396 + Pf6N2E2_2840 0.53 -0.5 3,330,396 + Pf6N2E2_2840 0.53 -1.7 3,330,396 + Pf6N2E2_2840 0.53 -0.3 3,330,396 + Pf6N2E2_2840 0.53 -1.5 3,330,396 + Pf6N2E2_2840 0.53 -1.4 3,330,396 + Pf6N2E2_2840 0.53 -1.0 3,330,397 - Pf6N2E2_2840 0.54 -0.4 3,330,409 - Pf6N2E2_2840 0.60 +3.1 3,330,631 - -2.3 3,330,657 + -1.0 3,330,657 + -0.2 3,330,749 + -2.8
Or see this region's nucleotide sequence