Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2822 and Pf6N2E2_2823 are separated by 261 nucleotides Pf6N2E2_2823 and Pf6N2E2_2824 are separated by 3 nucleotides
Pf6N2E2_2822 - Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family, at 3,308,633 to 3,309,265
_2822
Pf6N2E2_2823 - Multidrug efflux RND membrane fusion protein MexC, at 3,309,527 to 3,310,684
_2823
Pf6N2E2_2824 - RND efflux system, inner membrane transporter CmeB, at 3,310,688 to 3,313,852
_2824
Position (kb)
3309
3310
3311 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3308.528 kb on - strand at 3308.528 kb on - strand at 3308.550 kb on + strand at 3308.575 kb on + strand at 3308.576 kb on - strand at 3308.607 kb on - strand at 3308.666 kb on - strand at 3308.666 kb on - strand at 3308.739 kb on + strand, within Pf6N2E2_2822 at 3308.739 kb on + strand, within Pf6N2E2_2822 at 3308.739 kb on + strand, within Pf6N2E2_2822 at 3308.740 kb on - strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.920 kb on + strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.921 kb on - strand, within Pf6N2E2_2822 at 3308.939 kb on - strand, within Pf6N2E2_2822 at 3309.002 kb on - strand, within Pf6N2E2_2822 at 3309.002 kb on - strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.120 kb on + strand, within Pf6N2E2_2822 at 3309.121 kb on - strand, within Pf6N2E2_2822 at 3309.121 kb on - strand, within Pf6N2E2_2822 at 3309.271 kb on + strand at 3309.271 kb on + strand at 3309.272 kb on - strand at 3309.304 kb on + strand at 3309.305 kb on - strand at 3309.305 kb on - strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.315 kb on + strand at 3309.379 kb on + strand at 3309.379 kb on + strand at 3309.379 kb on + strand at 3309.383 kb on + strand at 3309.383 kb on + strand at 3309.384 kb on - strand at 3309.423 kb on + strand at 3309.427 kb on + strand at 3309.433 kb on + strand at 3309.441 kb on - strand at 3309.448 kb on + strand at 3309.448 kb on + strand at 3309.797 kb on + strand, within Pf6N2E2_2823 at 3309.818 kb on + strand, within Pf6N2E2_2823 at 3309.818 kb on + strand, within Pf6N2E2_2823 at 3309.819 kb on - strand, within Pf6N2E2_2823 at 3309.924 kb on - strand, within Pf6N2E2_2823 at 3309.992 kb on + strand, within Pf6N2E2_2823 at 3309.993 kb on - strand, within Pf6N2E2_2823 at 3310.004 kb on + strand, within Pf6N2E2_2823 at 3310.112 kb on + strand, within Pf6N2E2_2823 at 3310.113 kb on - strand, within Pf6N2E2_2823 at 3310.113 kb on - strand, within Pf6N2E2_2823 at 3310.238 kb on + strand, within Pf6N2E2_2823 at 3310.657 kb on + strand at 3310.658 kb on - strand at 3310.916 kb on + strand at 3310.917 kb on - strand at 3310.920 kb on + strand at 3310.920 kb on + strand at 3310.920 kb on + strand at 3310.921 kb on - strand at 3310.934 kb on - strand at 3311.156 kb on + strand, within Pf6N2E2_2824 at 3311.156 kb on + strand, within Pf6N2E2_2824 at 3311.156 kb on + strand, within Pf6N2E2_2824 at 3311.232 kb on - strand, within Pf6N2E2_2824 at 3311.386 kb on + strand, within Pf6N2E2_2824 at 3311.387 kb on - strand, within Pf6N2E2_2824 at 3311.387 kb on - strand, within Pf6N2E2_2824 at 3311.387 kb on - strand, within Pf6N2E2_2824 at 3311.510 kb on + strand, within Pf6N2E2_2824 at 3311.510 kb on + strand, within Pf6N2E2_2824 at 3311.510 kb on + strand, within Pf6N2E2_2824 at 3311.511 kb on - strand, within Pf6N2E2_2824 at 3311.511 kb on - strand, within Pf6N2E2_2824
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 3,308,528 - -1.2 3,308,528 - -0.6 3,308,550 + -2.0 3,308,575 + -1.9 3,308,576 - -3.9 3,308,607 - -1.1 3,308,666 - +3.0 3,308,666 - +3.3 3,308,739 + Pf6N2E2_2822 0.17 +2.9 3,308,739 + Pf6N2E2_2822 0.17 +3.0 3,308,739 + Pf6N2E2_2822 0.17 +2.5 3,308,740 - Pf6N2E2_2822 0.17 +2.6 3,308,920 + Pf6N2E2_2822 0.45 +3.2 3,308,920 + Pf6N2E2_2822 0.45 +3.6 3,308,920 + Pf6N2E2_2822 0.45 +3.6 3,308,920 + Pf6N2E2_2822 0.45 +2.7 3,308,920 + Pf6N2E2_2822 0.45 +3.5 3,308,920 + Pf6N2E2_2822 0.45 +2.7 3,308,921 - Pf6N2E2_2822 0.45 +3.0 3,308,921 - Pf6N2E2_2822 0.45 +2.7 3,308,921 - Pf6N2E2_2822 0.45 +2.5 3,308,921 - Pf6N2E2_2822 0.45 +2.6 3,308,939 - Pf6N2E2_2822 0.48 +3.1 3,309,002 - Pf6N2E2_2822 0.58 +2.3 3,309,002 - Pf6N2E2_2822 0.58 +1.5 3,309,120 + Pf6N2E2_2822 0.77 +2.2 3,309,120 + Pf6N2E2_2822 0.77 +1.7 3,309,120 + Pf6N2E2_2822 0.77 +3.0 3,309,120 + Pf6N2E2_2822 0.77 +2.9 3,309,121 - Pf6N2E2_2822 0.77 +1.9 3,309,121 - Pf6N2E2_2822 0.77 +1.9 3,309,271 + +2.5 3,309,271 + +1.7 3,309,272 - -3.4 3,309,304 + +2.4 3,309,305 - -2.8 3,309,305 - -4.4 3,309,315 + +2.5 3,309,315 + +2.6 3,309,315 + +1.6 3,309,315 + +2.1 3,309,315 + +2.2 3,309,379 + +1.6 3,309,379 + +1.6 3,309,379 + +1.2 3,309,383 + +2.8 3,309,383 + +0.5 3,309,384 - -4.5 3,309,423 + +2.6 3,309,427 + +1.8 3,309,433 + +1.3 3,309,441 - -3.4 3,309,448 + +1.1 3,309,448 + -0.5 3,309,797 + Pf6N2E2_2823 0.23 -2.5 3,309,818 + Pf6N2E2_2823 0.25 -3.7 3,309,818 + Pf6N2E2_2823 0.25 -1.5 3,309,819 - Pf6N2E2_2823 0.25 -2.4 3,309,924 - Pf6N2E2_2823 0.34 -2.4 3,309,992 + Pf6N2E2_2823 0.40 -3.4 3,309,993 - Pf6N2E2_2823 0.40 -2.2 3,310,004 + Pf6N2E2_2823 0.41 -2.2 3,310,112 + Pf6N2E2_2823 0.51 -1.1 3,310,113 - Pf6N2E2_2823 0.51 -0.5 3,310,113 - Pf6N2E2_2823 0.51 -2.4 3,310,238 + Pf6N2E2_2823 0.61 -2.2 3,310,657 + -3.3 3,310,658 - -0.9 3,310,916 + -2.7 3,310,917 - -2.8 3,310,920 + -2.6 3,310,920 + -3.3 3,310,920 + -1.8 3,310,921 - -2.5 3,310,934 - -1.2 3,311,156 + Pf6N2E2_2824 0.15 -4.3 3,311,156 + Pf6N2E2_2824 0.15 -2.7 3,311,156 + Pf6N2E2_2824 0.15 -1.7 3,311,232 - Pf6N2E2_2824 0.17 -3.0 3,311,386 + Pf6N2E2_2824 0.22 -3.5 3,311,387 - Pf6N2E2_2824 0.22 -1.7 3,311,387 - Pf6N2E2_2824 0.22 -2.4 3,311,387 - Pf6N2E2_2824 0.22 -2.8 3,311,510 + Pf6N2E2_2824 0.26 +0.3 3,311,510 + Pf6N2E2_2824 0.26 -2.2 3,311,510 + Pf6N2E2_2824 0.26 -4.2 3,311,511 - Pf6N2E2_2824 0.26 -0.9 3,311,511 - Pf6N2E2_2824 0.26 -1.2
Or see this region's nucleotide sequence