Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2345 and Pf6N2E2_2346 are separated by 142 nucleotides
Pf6N2E2_2345 - CHASE domain/PAS domain protein, at 2,777,197 to 2,779,581
_2345
Pf6N2E2_2346 - 23S rRNA (guanine-N-2-) -methyltransferase rlmL EC 2.1.1.-), at 2,779,724 to 2,781,994
_2346
Position (kb)
2779
2780
2781
2782 Strain fitness (log2 ratio)
-2
-1
0
1 at 2779.086 kb on - strand, within Pf6N2E2_2345 at 2779.106 kb on + strand, within Pf6N2E2_2345 at 2779.107 kb on - strand, within Pf6N2E2_2345 at 2779.107 kb on - strand, within Pf6N2E2_2345 at 2779.107 kb on - strand, within Pf6N2E2_2345 at 2779.121 kb on + strand, within Pf6N2E2_2345 at 2779.122 kb on - strand, within Pf6N2E2_2345 at 2779.280 kb on - strand, within Pf6N2E2_2345 at 2779.280 kb on - strand, within Pf6N2E2_2345 at 2779.280 kb on - strand, within Pf6N2E2_2345 at 2779.292 kb on + strand, within Pf6N2E2_2345 at 2779.488 kb on + strand at 2779.488 kb on + strand at 2779.489 kb on - strand at 2779.542 kb on + strand at 2779.542 kb on + strand at 2779.581 kb on + strand at 2779.638 kb on - strand at 2779.640 kb on - strand at 2779.679 kb on - strand at 2779.679 kb on - strand at 2779.869 kb on + strand at 2779.869 kb on + strand at 2779.869 kb on + strand at 2779.869 kb on + strand at 2779.870 kb on - strand at 2780.001 kb on + strand, within Pf6N2E2_2346 at 2780.180 kb on - strand, within Pf6N2E2_2346 at 2780.181 kb on + strand, within Pf6N2E2_2346 at 2780.404 kb on + strand, within Pf6N2E2_2346 at 2780.493 kb on + strand, within Pf6N2E2_2346 at 2780.507 kb on + strand, within Pf6N2E2_2346 at 2780.521 kb on - strand, within Pf6N2E2_2346 at 2780.541 kb on + strand, within Pf6N2E2_2346 at 2780.541 kb on + strand, within Pf6N2E2_2346 at 2780.562 kb on + strand, within Pf6N2E2_2346 at 2780.562 kb on + strand, within Pf6N2E2_2346 at 2780.562 kb on + strand, within Pf6N2E2_2346 at 2780.602 kb on + strand, within Pf6N2E2_2346 at 2780.603 kb on - strand, within Pf6N2E2_2346 at 2780.603 kb on - strand, within Pf6N2E2_2346 at 2780.728 kb on - strand, within Pf6N2E2_2346 at 2780.728 kb on - strand, within Pf6N2E2_2346 at 2780.728 kb on - strand, within Pf6N2E2_2346 at 2780.763 kb on + strand, within Pf6N2E2_2346 at 2780.764 kb on - strand, within Pf6N2E2_2346 at 2780.886 kb on + strand, within Pf6N2E2_2346 at 2780.886 kb on + strand, within Pf6N2E2_2346 at 2780.887 kb on - strand, within Pf6N2E2_2346 at 2780.887 kb on - strand, within Pf6N2E2_2346 at 2780.887 kb on - strand, within Pf6N2E2_2346 at 2780.998 kb on - strand, within Pf6N2E2_2346 at 2781.003 kb on + strand, within Pf6N2E2_2346 at 2781.003 kb on + strand, within Pf6N2E2_2346 at 2781.004 kb on - strand, within Pf6N2E2_2346 at 2781.114 kb on + strand, within Pf6N2E2_2346 at 2781.114 kb on + strand, within Pf6N2E2_2346 at 2781.114 kb on + strand, within Pf6N2E2_2346 at 2781.115 kb on - strand, within Pf6N2E2_2346 at 2781.115 kb on - strand, within Pf6N2E2_2346 at 2781.359 kb on + strand, within Pf6N2E2_2346 at 2781.360 kb on - strand, within Pf6N2E2_2346 at 2781.768 kb on + strand at 2782.122 kb on - strand at 2782.132 kb on - strand at 2782.195 kb on + strand at 2782.199 kb on - strand at 2782.240 kb on + strand at 2782.240 kb on + strand at 2782.240 kb on + strand at 2782.266 kb on + strand at 2782.266 kb on + strand at 2782.267 kb on - strand at 2782.267 kb on - strand at 2782.306 kb on + strand at 2782.306 kb on + strand at 2782.307 kb on - strand at 2782.307 kb on - strand at 2782.311 kb on - strand at 2782.327 kb on + strand at 2782.327 kb on + strand at 2782.342 kb on - strand at 2782.380 kb on + strand at 2782.497 kb on + strand at 2782.498 kb on - strand at 2782.498 kb on - strand at 2782.498 kb on - strand at 2782.498 kb on - strand at 2782.525 kb on + strand at 2782.526 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.538 kb on - strand at 2782.566 kb on + strand at 2782.566 kb on + strand at 2782.575 kb on - strand at 2782.581 kb on - strand at 2782.594 kb on + strand at 2782.594 kb on + strand at 2782.618 kb on + strand at 2782.619 kb on - strand at 2782.619 kb on - strand at 2782.619 kb on - strand at 2782.623 kb on + strand at 2782.687 kb on + strand at 2782.711 kb on + strand at 2782.711 kb on + strand at 2782.712 kb on - strand at 2782.751 kb on + strand at 2782.751 kb on + strand at 2782.752 kb on - strand at 2782.752 kb on - strand at 2782.752 kb on - strand at 2782.856 kb on + strand at 2782.856 kb on + strand at 2782.857 kb on - strand at 2782.857 kb on - strand at 2782.870 kb on + strand at 2782.871 kb on - strand at 2782.871 kb on - strand at 2782.871 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 2,779,086 - Pf6N2E2_2345 0.79 +0.4 2,779,106 + Pf6N2E2_2345 0.80 +1.4 2,779,107 - Pf6N2E2_2345 0.80 -0.9 2,779,107 - Pf6N2E2_2345 0.80 -1.1 2,779,107 - Pf6N2E2_2345 0.80 -0.1 2,779,121 + Pf6N2E2_2345 0.81 +0.9 2,779,122 - Pf6N2E2_2345 0.81 +1.2 2,779,280 - Pf6N2E2_2345 0.87 -1.4 2,779,280 - Pf6N2E2_2345 0.87 -0.9 2,779,280 - Pf6N2E2_2345 0.87 +0.6 2,779,292 + Pf6N2E2_2345 0.88 +0.2 2,779,488 + +0.2 2,779,488 + -0.4 2,779,489 - +0.6 2,779,542 + -2.1 2,779,542 + -0.4 2,779,581 + -1.1 2,779,638 - -2.0 2,779,640 - +0.0 2,779,679 - -0.0 2,779,679 - +0.8 2,779,869 + +0.4 2,779,869 + +0.2 2,779,869 + +0.2 2,779,869 + -0.1 2,779,870 - +1.0 2,780,001 + Pf6N2E2_2346 0.12 +1.7 2,780,180 - Pf6N2E2_2346 0.20 -0.2 2,780,181 + Pf6N2E2_2346 0.20 +0.2 2,780,404 + Pf6N2E2_2346 0.30 -0.2 2,780,493 + Pf6N2E2_2346 0.34 +0.8 2,780,507 + Pf6N2E2_2346 0.34 -0.9 2,780,521 - Pf6N2E2_2346 0.35 +0.2 2,780,541 + Pf6N2E2_2346 0.36 +0.5 2,780,541 + Pf6N2E2_2346 0.36 +1.1 2,780,562 + Pf6N2E2_2346 0.37 -0.5 2,780,562 + Pf6N2E2_2346 0.37 -0.2 2,780,562 + Pf6N2E2_2346 0.37 -0.6 2,780,602 + Pf6N2E2_2346 0.39 +0.9 2,780,603 - Pf6N2E2_2346 0.39 +1.0 2,780,603 - Pf6N2E2_2346 0.39 -0.1 2,780,728 - Pf6N2E2_2346 0.44 +1.2 2,780,728 - Pf6N2E2_2346 0.44 +0.3 2,780,728 - Pf6N2E2_2346 0.44 -0.1 2,780,763 + Pf6N2E2_2346 0.46 +1.2 2,780,764 - Pf6N2E2_2346 0.46 +0.3 2,780,886 + Pf6N2E2_2346 0.51 +0.7 2,780,886 + Pf6N2E2_2346 0.51 +1.4 2,780,887 - Pf6N2E2_2346 0.51 +0.9 2,780,887 - Pf6N2E2_2346 0.51 +0.5 2,780,887 - Pf6N2E2_2346 0.51 +0.1 2,780,998 - Pf6N2E2_2346 0.56 +0.4 2,781,003 + Pf6N2E2_2346 0.56 -0.2 2,781,003 + Pf6N2E2_2346 0.56 +0.6 2,781,004 - Pf6N2E2_2346 0.56 -0.7 2,781,114 + Pf6N2E2_2346 0.61 +1.0 2,781,114 + Pf6N2E2_2346 0.61 +1.4 2,781,114 + Pf6N2E2_2346 0.61 +0.5 2,781,115 - Pf6N2E2_2346 0.61 -0.1 2,781,115 - Pf6N2E2_2346 0.61 +0.4 2,781,359 + Pf6N2E2_2346 0.72 +0.7 2,781,360 - Pf6N2E2_2346 0.72 +1.0 2,781,768 + +0.0 2,782,122 - -0.4 2,782,132 - +0.8 2,782,195 + +0.0 2,782,199 - -1.1 2,782,240 + +0.8 2,782,240 + +0.6 2,782,240 + +0.4 2,782,266 + -1.1 2,782,266 + +0.8 2,782,267 - -0.6 2,782,267 - +0.8 2,782,306 + -0.2 2,782,306 + -0.6 2,782,307 - -0.1 2,782,307 - +0.8 2,782,311 - -0.5 2,782,327 + +0.5 2,782,327 + -0.1 2,782,342 - +0.9 2,782,380 + +0.5 2,782,497 + -0.6 2,782,498 - +1.1 2,782,498 - -0.3 2,782,498 - -0.3 2,782,498 - +0.2 2,782,525 + +0.7 2,782,526 - -1.0 2,782,538 - +0.5 2,782,538 - -0.9 2,782,538 - +0.7 2,782,538 - -0.5 2,782,538 - +0.7 2,782,566 + -0.4 2,782,566 + +0.0 2,782,575 - -1.4 2,782,581 - +0.0 2,782,594 + -1.9 2,782,594 + +0.3 2,782,618 + +1.0 2,782,619 - +0.2 2,782,619 - -0.2 2,782,619 - +0.8 2,782,623 + -0.7 2,782,687 + -1.3 2,782,711 + +0.4 2,782,711 + -0.1 2,782,712 - -0.3 2,782,751 + -0.4 2,782,751 + +0.6 2,782,752 - +1.0 2,782,752 - +0.3 2,782,752 - -1.1 2,782,856 + +0.5 2,782,856 + +0.0 2,782,857 - -0.7 2,782,857 - +1.0 2,782,870 + +0.2 2,782,871 - -0.1 2,782,871 - -1.4 2,782,871 - +0.6
Or see this region's nucleotide sequence