Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1932 and Pf6N2E2_1933 are separated by 202 nucleotides Pf6N2E2_1933 and Pf6N2E2_1934 are separated by 55 nucleotides Pf6N2E2_1934 and Pf6N2E2_1935 are separated by 63 nucleotides
Pf6N2E2_1932 - Transcriptional regulator, IclR family, at 2,288,821 to 2,289,564
_1932
Pf6N2E2_1933 - TcuR: regulates tcuABC genes used in utilization of tricarballylate, at 2,289,767 to 2,290,699
_1933
Pf6N2E2_1934 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,290,755 to 2,291,549
_1934
Pf6N2E2_1935 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,291,613 to 2,292,257
_1935
Position (kb)
2289
2290
2291 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2288.961 kb on - strand, within Pf6N2E2_1932 at 2288.961 kb on - strand, within Pf6N2E2_1932 at 2289.014 kb on - strand, within Pf6N2E2_1932 at 2289.149 kb on + strand, within Pf6N2E2_1932 at 2289.149 kb on + strand, within Pf6N2E2_1932 at 2289.149 kb on + strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.150 kb on - strand, within Pf6N2E2_1932 at 2289.429 kb on - strand, within Pf6N2E2_1932 at 2289.609 kb on - strand at 2289.674 kb on - strand at 2289.674 kb on - strand at 2289.674 kb on - strand at 2289.676 kb on + strand at 2289.677 kb on - strand at 2289.677 kb on - strand at 2289.677 kb on - strand at 2289.677 kb on - strand at 2289.677 kb on - strand at 2289.823 kb on + strand at 2289.824 kb on - strand at 2289.824 kb on - strand at 2289.956 kb on - strand, within Pf6N2E2_1933 at 2289.980 kb on - strand, within Pf6N2E2_1933 at 2290.127 kb on + strand, within Pf6N2E2_1933 at 2290.128 kb on - strand, within Pf6N2E2_1933 at 2290.128 kb on - strand, within Pf6N2E2_1933 at 2290.130 kb on - strand, within Pf6N2E2_1933 at 2290.223 kb on + strand, within Pf6N2E2_1933 at 2290.336 kb on + strand, within Pf6N2E2_1933 at 2290.337 kb on - strand, within Pf6N2E2_1933 at 2290.553 kb on + strand, within Pf6N2E2_1933 at 2290.664 kb on + strand at 2290.713 kb on + strand at 2290.713 kb on + strand at 2290.757 kb on - strand at 2290.792 kb on + strand at 2290.792 kb on + strand at 2290.793 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.894 kb on + strand, within Pf6N2E2_1934 at 2290.895 kb on - strand, within Pf6N2E2_1934 at 2291.010 kb on + strand, within Pf6N2E2_1934 at 2291.011 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.083 kb on - strand, within Pf6N2E2_1934 at 2291.098 kb on + strand, within Pf6N2E2_1934 at 2291.099 kb on - strand, within Pf6N2E2_1934 at 2291.135 kb on + strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.137 kb on + strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.266 kb on - strand, within Pf6N2E2_1934 at 2291.304 kb on - strand, within Pf6N2E2_1934 at 2291.500 kb on - strand at 2291.509 kb on - strand at 2291.529 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 2,288,961 - Pf6N2E2_1932 0.19 +0.6 2,288,961 - Pf6N2E2_1932 0.19 -1.9 2,289,014 - Pf6N2E2_1932 0.26 -0.1 2,289,149 + Pf6N2E2_1932 0.44 -0.4 2,289,149 + Pf6N2E2_1932 0.44 +0.2 2,289,149 + Pf6N2E2_1932 0.44 -1.5 2,289,150 - Pf6N2E2_1932 0.44 -0.9 2,289,150 - Pf6N2E2_1932 0.44 -1.4 2,289,150 - Pf6N2E2_1932 0.44 -0.4 2,289,150 - Pf6N2E2_1932 0.44 +0.0 2,289,150 - Pf6N2E2_1932 0.44 +0.3 2,289,150 - Pf6N2E2_1932 0.44 -0.3 2,289,150 - Pf6N2E2_1932 0.44 +0.5 2,289,429 - Pf6N2E2_1932 0.82 +0.1 2,289,609 - +0.3 2,289,674 - +0.7 2,289,674 - -0.4 2,289,674 - +0.7 2,289,676 + +0.2 2,289,677 - -0.4 2,289,677 - -0.1 2,289,677 - -0.5 2,289,677 - -1.7 2,289,677 - +0.1 2,289,823 + +0.2 2,289,824 - +0.7 2,289,824 - -0.8 2,289,956 - Pf6N2E2_1933 0.20 -1.0 2,289,980 - Pf6N2E2_1933 0.23 -2.0 2,290,127 + Pf6N2E2_1933 0.39 -1.7 2,290,128 - Pf6N2E2_1933 0.39 -1.0 2,290,128 - Pf6N2E2_1933 0.39 -1.0 2,290,130 - Pf6N2E2_1933 0.39 -1.1 2,290,223 + Pf6N2E2_1933 0.49 -0.8 2,290,336 + Pf6N2E2_1933 0.61 -0.8 2,290,337 - Pf6N2E2_1933 0.61 +1.3 2,290,553 + Pf6N2E2_1933 0.84 +0.8 2,290,664 + +0.3 2,290,713 + -1.2 2,290,713 + -1.4 2,290,757 - -1.1 2,290,792 + +0.6 2,290,792 + +0.2 2,290,793 - -0.6 2,290,814 - -0.4 2,290,814 - +0.5 2,290,814 - -0.0 2,290,814 - +1.1 2,290,894 + Pf6N2E2_1934 0.17 -0.7 2,290,895 - Pf6N2E2_1934 0.18 +0.1 2,291,010 + Pf6N2E2_1934 0.32 +1.5 2,291,011 - Pf6N2E2_1934 0.32 +1.8 2,291,056 - Pf6N2E2_1934 0.38 -1.3 2,291,056 - Pf6N2E2_1934 0.38 +0.1 2,291,083 - Pf6N2E2_1934 0.41 -0.6 2,291,098 + Pf6N2E2_1934 0.43 -0.4 2,291,099 - Pf6N2E2_1934 0.43 +0.7 2,291,135 + Pf6N2E2_1934 0.48 -0.2 2,291,136 - Pf6N2E2_1934 0.48 +0.5 2,291,136 - Pf6N2E2_1934 0.48 -0.7 2,291,137 + Pf6N2E2_1934 0.48 -0.6 2,291,138 - Pf6N2E2_1934 0.48 -0.9 2,291,138 - Pf6N2E2_1934 0.48 -1.5 2,291,138 - Pf6N2E2_1934 0.48 -0.2 2,291,138 - Pf6N2E2_1934 0.48 +0.1 2,291,138 - Pf6N2E2_1934 0.48 -0.3 2,291,266 - Pf6N2E2_1934 0.64 -0.8 2,291,304 - Pf6N2E2_1934 0.69 +0.2 2,291,500 - -0.2 2,291,509 - +1.2 2,291,529 - +0.0 2,291,553 - -0.6 2,291,553 - +0.4 2,291,553 - +1.3 2,291,553 - +0.3 2,291,553 - +0.4 2,291,553 - -0.2
Or see this region's nucleotide sequence