Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1516

Experiment: Fusidic 0.3 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1515 and Pf6N2E2_1516 are separated by 232 nucleotidesPf6N2E2_1516 and Pf6N2E2_1517 are separated by 130 nucleotides Pf6N2E2_1515 - Cell division protein FtsH (EC 3.4.24.-), at 1,769,751 to 1,771,577 _1515 Pf6N2E2_1516 - transcriptional regulator, Crp/Fnr family, at 1,771,810 to 1,772,562 _1516 Pf6N2E2_1517 - Arginine/ornithine antiporter ArcD, at 1,772,693 to 1,774,186 _1517 Position (kb) 1771 1772 1773Strain fitness (log2 ratio) -2 -1 0 1at 1770.831 kb on - strand, within Pf6N2E2_1515at 1770.970 kb on + strand, within Pf6N2E2_1515at 1770.971 kb on - strand, within Pf6N2E2_1515at 1770.971 kb on - strand, within Pf6N2E2_1515at 1770.971 kb on - strand, within Pf6N2E2_1515at 1770.971 kb on - strand, within Pf6N2E2_1515at 1770.971 kb on - strand, within Pf6N2E2_1515at 1770.999 kb on + strand, within Pf6N2E2_1515at 1771.059 kb on - strand, within Pf6N2E2_1515at 1771.064 kb on - strand, within Pf6N2E2_1515at 1771.099 kb on - strand, within Pf6N2E2_1515at 1771.101 kb on - strand, within Pf6N2E2_1515at 1771.101 kb on - strand, within Pf6N2E2_1515at 1771.203 kb on + strand, within Pf6N2E2_1515at 1771.332 kb on + strand, within Pf6N2E2_1515at 1771.333 kb on - strand, within Pf6N2E2_1515at 1771.333 kb on - strand, within Pf6N2E2_1515at 1771.333 kb on - strand, within Pf6N2E2_1515at 1771.429 kb on - strandat 1771.429 kb on - strandat 1771.666 kb on + strandat 1771.666 kb on + strandat 1771.666 kb on + strandat 1771.666 kb on + strandat 1771.667 kb on - strandat 1771.667 kb on - strandat 1771.667 kb on - strandat 1771.667 kb on - strandat 1771.778 kb on + strandat 1771.779 kb on - strandat 1771.779 kb on - strandat 1771.779 kb on - strandat 1771.793 kb on + strandat 1771.794 kb on - strandat 1772.009 kb on - strand, within Pf6N2E2_1516at 1772.009 kb on - strand, within Pf6N2E2_1516at 1772.052 kb on - strand, within Pf6N2E2_1516at 1772.211 kb on - strand, within Pf6N2E2_1516at 1772.211 kb on - strand, within Pf6N2E2_1516at 1772.325 kb on - strand, within Pf6N2E2_1516at 1772.347 kb on - strand, within Pf6N2E2_1516at 1772.360 kb on - strand, within Pf6N2E2_1516at 1772.588 kb on + strandat 1772.713 kb on + strandat 1772.714 kb on - strandat 1773.077 kb on + strand, within Pf6N2E2_1517at 1773.078 kb on - strand, within Pf6N2E2_1517at 1773.108 kb on - strand, within Pf6N2E2_1517at 1773.108 kb on - strand, within Pf6N2E2_1517at 1773.133 kb on + strand, within Pf6N2E2_1517at 1773.133 kb on + strand, within Pf6N2E2_1517at 1773.136 kb on + strand, within Pf6N2E2_1517at 1773.137 kb on - strand, within Pf6N2E2_1517at 1773.414 kb on + strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517at 1773.415 kb on - strand, within Pf6N2E2_1517

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml
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1,770,831 - Pf6N2E2_1515 0.59 -0.5
1,770,970 + Pf6N2E2_1515 0.67 +0.7
1,770,971 - Pf6N2E2_1515 0.67 -0.0
1,770,971 - Pf6N2E2_1515 0.67 +0.4
1,770,971 - Pf6N2E2_1515 0.67 +1.4
1,770,971 - Pf6N2E2_1515 0.67 -0.3
1,770,971 - Pf6N2E2_1515 0.67 +0.2
1,770,999 + Pf6N2E2_1515 0.68 +0.9
1,771,059 - Pf6N2E2_1515 0.72 +0.3
1,771,064 - Pf6N2E2_1515 0.72 -1.2
1,771,099 - Pf6N2E2_1515 0.74 +0.7
1,771,101 - Pf6N2E2_1515 0.74 -0.0
1,771,101 - Pf6N2E2_1515 0.74 -0.6
1,771,203 + Pf6N2E2_1515 0.79 -1.1
1,771,332 + Pf6N2E2_1515 0.87 +1.3
1,771,333 - Pf6N2E2_1515 0.87 +1.5
1,771,333 - Pf6N2E2_1515 0.87 +0.3
1,771,333 - Pf6N2E2_1515 0.87 -1.3
1,771,429 - +0.2
1,771,429 - +0.6
1,771,666 + +0.5
1,771,666 + +0.5
1,771,666 + -1.6
1,771,666 + +0.3
1,771,667 - -0.4
1,771,667 - +1.7
1,771,667 - -0.7
1,771,667 - +1.4
1,771,778 + +0.2
1,771,779 - -0.4
1,771,779 - -1.7
1,771,779 - +0.5
1,771,793 + +0.7
1,771,794 - +0.4
1,772,009 - Pf6N2E2_1516 0.26 -0.3
1,772,009 - Pf6N2E2_1516 0.26 -0.7
1,772,052 - Pf6N2E2_1516 0.32 +0.4
1,772,211 - Pf6N2E2_1516 0.53 -1.4
1,772,211 - Pf6N2E2_1516 0.53 -1.4
1,772,325 - Pf6N2E2_1516 0.68 -0.3
1,772,347 - Pf6N2E2_1516 0.71 -0.3
1,772,360 - Pf6N2E2_1516 0.73 -2.1
1,772,588 + -0.8
1,772,713 + +0.0
1,772,714 - +0.9
1,773,077 + Pf6N2E2_1517 0.26 +0.3
1,773,078 - Pf6N2E2_1517 0.26 +0.9
1,773,108 - Pf6N2E2_1517 0.28 +1.4
1,773,108 - Pf6N2E2_1517 0.28 -0.1
1,773,133 + Pf6N2E2_1517 0.29 +1.1
1,773,133 + Pf6N2E2_1517 0.29 +0.9
1,773,136 + Pf6N2E2_1517 0.30 -2.1
1,773,137 - Pf6N2E2_1517 0.30 +0.1
1,773,414 + Pf6N2E2_1517 0.48 -0.1
1,773,415 - Pf6N2E2_1517 0.48 +0.9
1,773,415 - Pf6N2E2_1517 0.48 -0.1
1,773,415 - Pf6N2E2_1517 0.48 -0.9
1,773,415 - Pf6N2E2_1517 0.48 -0.5

Or see this region's nucleotide sequence