Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1343 and Pf6N2E2_1344 are separated by 121 nucleotides Pf6N2E2_1344 and Pf6N2E2_1345 are separated by 51 nucleotides
Pf6N2E2_1343 - Transcriptional regulator, at 1,579,210 to 1,580,124
_1343
Pf6N2E2_1344 - Pirin-related protein, at 1,580,246 to 1,581,085
_1344
Pf6N2E2_1345 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 1,581,137 to 1,582,213
_1345
Position (kb)
1580
1581
1582 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1579.341 kb on - strand, within Pf6N2E2_1343 at 1579.610 kb on + strand, within Pf6N2E2_1343 at 1579.610 kb on + strand, within Pf6N2E2_1343 at 1579.611 kb on - strand, within Pf6N2E2_1343 at 1579.668 kb on - strand, within Pf6N2E2_1343 at 1579.928 kb on + strand, within Pf6N2E2_1343 at 1579.928 kb on + strand, within Pf6N2E2_1343 at 1580.169 kb on + strand at 1580.199 kb on + strand at 1580.200 kb on - strand at 1580.200 kb on - strand at 1580.200 kb on - strand at 1580.453 kb on + strand, within Pf6N2E2_1344 at 1580.453 kb on + strand, within Pf6N2E2_1344 at 1580.453 kb on + strand, within Pf6N2E2_1344 at 1580.453 kb on + strand, within Pf6N2E2_1344 at 1580.453 kb on + strand, within Pf6N2E2_1344 at 1580.453 kb on + strand, within Pf6N2E2_1344 at 1580.454 kb on - strand, within Pf6N2E2_1344 at 1580.506 kb on - strand, within Pf6N2E2_1344 at 1580.550 kb on + strand, within Pf6N2E2_1344 at 1580.651 kb on + strand, within Pf6N2E2_1344 at 1580.709 kb on - strand, within Pf6N2E2_1344 at 1580.709 kb on - strand, within Pf6N2E2_1344 at 1580.753 kb on + strand, within Pf6N2E2_1344 at 1580.753 kb on + strand, within Pf6N2E2_1344 at 1580.753 kb on + strand, within Pf6N2E2_1344 at 1580.753 kb on + strand, within Pf6N2E2_1344 at 1580.754 kb on - strand, within Pf6N2E2_1344 at 1580.793 kb on - strand, within Pf6N2E2_1344 at 1580.794 kb on + strand, within Pf6N2E2_1344 at 1580.794 kb on + strand, within Pf6N2E2_1344 at 1580.795 kb on - strand, within Pf6N2E2_1344 at 1580.987 kb on + strand, within Pf6N2E2_1344 at 1580.987 kb on + strand, within Pf6N2E2_1344 at 1580.987 kb on + strand, within Pf6N2E2_1344 at 1580.991 kb on - strand, within Pf6N2E2_1344 at 1580.991 kb on - strand, within Pf6N2E2_1344 at 1581.107 kb on - strand at 1581.126 kb on - strand at 1581.126 kb on - strand at 1581.275 kb on + strand, within Pf6N2E2_1345 at 1581.276 kb on - strand, within Pf6N2E2_1345 at 1581.279 kb on - strand, within Pf6N2E2_1345 at 1581.539 kb on + strand, within Pf6N2E2_1345 at 1581.539 kb on + strand, within Pf6N2E2_1345 at 1581.540 kb on - strand, within Pf6N2E2_1345 at 1581.564 kb on - strand, within Pf6N2E2_1345 at 1581.564 kb on - strand, within Pf6N2E2_1345 at 1581.686 kb on + strand, within Pf6N2E2_1345 at 1581.848 kb on + strand, within Pf6N2E2_1345 at 1581.849 kb on - strand, within Pf6N2E2_1345 at 1581.927 kb on - strand, within Pf6N2E2_1345 at 1581.927 kb on - strand, within Pf6N2E2_1345 at 1581.998 kb on + strand, within Pf6N2E2_1345 at 1581.999 kb on - strand, within Pf6N2E2_1345 at 1582.066 kb on - strand, within Pf6N2E2_1345 at 1582.066 kb on - strand, within Pf6N2E2_1345
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 1,579,341 - Pf6N2E2_1343 0.14 +0.3 1,579,610 + Pf6N2E2_1343 0.44 +0.6 1,579,610 + Pf6N2E2_1343 0.44 +0.3 1,579,611 - Pf6N2E2_1343 0.44 +0.5 1,579,668 - Pf6N2E2_1343 0.50 +1.7 1,579,928 + Pf6N2E2_1343 0.78 -0.1 1,579,928 + Pf6N2E2_1343 0.78 -0.1 1,580,169 + +0.9 1,580,199 + +0.5 1,580,200 - +0.4 1,580,200 - -0.6 1,580,200 - +0.5 1,580,453 + Pf6N2E2_1344 0.25 +0.9 1,580,453 + Pf6N2E2_1344 0.25 +0.4 1,580,453 + Pf6N2E2_1344 0.25 -0.8 1,580,453 + Pf6N2E2_1344 0.25 +0.1 1,580,453 + Pf6N2E2_1344 0.25 +0.3 1,580,453 + Pf6N2E2_1344 0.25 +0.7 1,580,454 - Pf6N2E2_1344 0.25 -0.0 1,580,506 - Pf6N2E2_1344 0.31 -0.1 1,580,550 + Pf6N2E2_1344 0.36 +0.9 1,580,651 + Pf6N2E2_1344 0.48 +0.3 1,580,709 - Pf6N2E2_1344 0.55 +0.0 1,580,709 - Pf6N2E2_1344 0.55 +0.0 1,580,753 + Pf6N2E2_1344 0.60 +1.4 1,580,753 + Pf6N2E2_1344 0.60 +0.6 1,580,753 + Pf6N2E2_1344 0.60 +1.2 1,580,753 + Pf6N2E2_1344 0.60 -3.0 1,580,754 - Pf6N2E2_1344 0.60 -0.3 1,580,793 - Pf6N2E2_1344 0.65 -1.7 1,580,794 + Pf6N2E2_1344 0.65 +0.5 1,580,794 + Pf6N2E2_1344 0.65 -0.7 1,580,795 - Pf6N2E2_1344 0.65 -0.4 1,580,987 + Pf6N2E2_1344 0.88 +0.6 1,580,987 + Pf6N2E2_1344 0.88 +0.2 1,580,987 + Pf6N2E2_1344 0.88 +1.0 1,580,991 - Pf6N2E2_1344 0.89 +1.3 1,580,991 - Pf6N2E2_1344 0.89 +1.4 1,581,107 - +0.5 1,581,126 - +0.6 1,581,126 - +1.1 1,581,275 + Pf6N2E2_1345 0.13 +0.4 1,581,276 - Pf6N2E2_1345 0.13 +0.3 1,581,279 - Pf6N2E2_1345 0.13 +0.2 1,581,539 + Pf6N2E2_1345 0.37 -0.1 1,581,539 + Pf6N2E2_1345 0.37 -0.0 1,581,540 - Pf6N2E2_1345 0.37 -0.6 1,581,564 - Pf6N2E2_1345 0.40 +0.5 1,581,564 - Pf6N2E2_1345 0.40 -0.4 1,581,686 + Pf6N2E2_1345 0.51 +0.9 1,581,848 + Pf6N2E2_1345 0.66 +0.5 1,581,849 - Pf6N2E2_1345 0.66 +1.0 1,581,927 - Pf6N2E2_1345 0.73 -2.1 1,581,927 - Pf6N2E2_1345 0.73 +0.9 1,581,998 + Pf6N2E2_1345 0.80 -0.1 1,581,999 - Pf6N2E2_1345 0.80 +0.3 1,582,066 - Pf6N2E2_1345 0.86 -0.4 1,582,066 - Pf6N2E2_1345 0.86 -0.7
Or see this region's nucleotide sequence