Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02980 and IAI47_02985 are separated by 20 nucleotides
IAI47_02980: IAI47_02980 - diguanylate cyclase, at 616,164 to 617,585
_02980
IAI47_02985: IAI47_02985 - D-alanine--D-alanine ligase, at 617,606 to 618,703
_02985
Position (kb) 617
618
619 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 616.634 kb on + strand, within IAI47_02980 at 616.634 kb on + strand, within IAI47_02980 at 616.635 kb on - strand, within IAI47_02980 at 616.777 kb on + strand, within IAI47_02980 at 616.777 kb on + strand, within IAI47_02980 at 616.778 kb on - strand, within IAI47_02980 at 616.778 kb on - strand, within IAI47_02980 at 616.778 kb on - strand, within IAI47_02980 at 616.793 kb on + strand, within IAI47_02980 at 616.793 kb on + strand, within IAI47_02980 at 616.912 kb on + strand, within IAI47_02980 at 616.912 kb on + strand, within IAI47_02980 at 616.912 kb on + strand, within IAI47_02980 at 616.913 kb on - strand, within IAI47_02980 at 616.913 kb on - strand, within IAI47_02980 at 616.934 kb on + strand, within IAI47_02980 at 616.934 kb on + strand, within IAI47_02980 at 616.935 kb on - strand, within IAI47_02980 at 616.935 kb on - strand, within IAI47_02980 at 616.985 kb on + strand, within IAI47_02980 at 616.986 kb on - strand, within IAI47_02980 at 616.986 kb on - strand, within IAI47_02980 at 616.994 kb on + strand, within IAI47_02980 at 616.994 kb on + strand, within IAI47_02980 at 616.995 kb on - strand, within IAI47_02980 at 617.067 kb on + strand, within IAI47_02980 at 617.068 kb on - strand, within IAI47_02980 at 617.104 kb on + strand, within IAI47_02980 at 617.104 kb on + strand, within IAI47_02980 at 617.104 kb on + strand, within IAI47_02980 at 617.104 kb on + strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.105 kb on - strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.106 kb on + strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.107 kb on - strand, within IAI47_02980 at 617.129 kb on + strand, within IAI47_02980 at 617.130 kb on - strand, within IAI47_02980 at 617.130 kb on - strand, within IAI47_02980 at 617.144 kb on + strand, within IAI47_02980 at 617.145 kb on - strand, within IAI47_02980 at 617.145 kb on - strand, within IAI47_02980 at 617.145 kb on - strand, within IAI47_02980 at 617.176 kb on - strand, within IAI47_02980 at 617.176 kb on - strand, within IAI47_02980 at 617.176 kb on - strand, within IAI47_02980 at 617.189 kb on + strand, within IAI47_02980 at 617.190 kb on - strand, within IAI47_02980 at 617.190 kb on - strand, within IAI47_02980 at 617.264 kb on + strand, within IAI47_02980 at 617.340 kb on + strand, within IAI47_02980 at 617.341 kb on - strand, within IAI47_02980 at 617.341 kb on - strand, within IAI47_02980 at 617.403 kb on - strand, within IAI47_02980 at 617.403 kb on - strand, within IAI47_02980 at 617.438 kb on + strand, within IAI47_02980 at 617.439 kb on - strand, within IAI47_02980 at 617.439 kb on - strand, within IAI47_02980 at 617.511 kb on + strand at 617.512 kb on - strand at 617.512 kb on - strand at 617.556 kb on + strand at 617.556 kb on + strand at 617.556 kb on + strand at 617.557 kb on - strand at 617.557 kb on - strand at 617.557 kb on - strand at 617.557 kb on - strand at 617.557 kb on - strand at 617.557 kb on - strand at 617.663 kb on + strand at 617.663 kb on + strand at 617.663 kb on + strand at 617.664 kb on - strand at 617.721 kb on + strand, within IAI47_02985 at 617.721 kb on + strand, within IAI47_02985 at 617.721 kb on + strand, within IAI47_02985 at 617.722 kb on - strand, within IAI47_02985 at 617.737 kb on + strand, within IAI47_02985 at 617.737 kb on + strand, within IAI47_02985 at 617.738 kb on - strand, within IAI47_02985 at 617.833 kb on + strand, within IAI47_02985 at 617.923 kb on + strand, within IAI47_02985 at 617.924 kb on - strand, within IAI47_02985 at 617.924 kb on - strand, within IAI47_02985 at 617.924 kb on - strand, within IAI47_02985 at 617.924 kb on - strand, within IAI47_02985 at 617.924 kb on - strand, within IAI47_02985 at 617.924 kb on - strand, within IAI47_02985 at 617.926 kb on - strand, within IAI47_02985 at 617.926 kb on - strand, within IAI47_02985 at 617.937 kb on + strand, within IAI47_02985 at 617.937 kb on + strand, within IAI47_02985 at 617.937 kb on + strand, within IAI47_02985 at 617.937 kb on + strand, within IAI47_02985 at 617.943 kb on + strand, within IAI47_02985 at 617.944 kb on - strand, within IAI47_02985 at 617.944 kb on - strand, within IAI47_02985 at 617.944 kb on - strand, within IAI47_02985 at 617.944 kb on - strand, within IAI47_02985 at 617.944 kb on - strand, within IAI47_02985 at 618.032 kb on + strand, within IAI47_02985 at 618.033 kb on - strand, within IAI47_02985 at 618.050 kb on + strand, within IAI47_02985 at 618.051 kb on - strand, within IAI47_02985 at 618.051 kb on - strand, within IAI47_02985 at 618.106 kb on + strand, within IAI47_02985 at 618.106 kb on + strand, within IAI47_02985 at 618.106 kb on + strand, within IAI47_02985 at 618.106 kb on + strand, within IAI47_02985 at 618.107 kb on - strand, within IAI47_02985 at 618.107 kb on - strand, within IAI47_02985 at 618.107 kb on - strand, within IAI47_02985 at 618.107 kb on - strand, within IAI47_02985 at 618.110 kb on + strand, within IAI47_02985 at 618.110 kb on + strand, within IAI47_02985 at 618.110 kb on + strand, within IAI47_02985 at 618.110 kb on + strand, within IAI47_02985 at 618.110 kb on + strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.111 kb on - strand, within IAI47_02985 at 618.155 kb on - strand, within IAI47_02985 at 618.155 kb on - strand, within IAI47_02985 at 618.155 kb on - strand, within IAI47_02985 at 618.158 kb on + strand, within IAI47_02985 at 618.158 kb on + strand, within IAI47_02985 at 618.159 kb on - strand, within IAI47_02985 at 618.260 kb on + strand, within IAI47_02985 at 618.260 kb on + strand, within IAI47_02985 at 618.261 kb on - strand, within IAI47_02985 at 618.261 kb on - strand, within IAI47_02985 at 618.329 kb on + strand, within IAI47_02985 at 618.329 kb on + strand, within IAI47_02985 at 618.340 kb on + strand, within IAI47_02985 at 618.340 kb on + strand, within IAI47_02985 at 618.340 kb on + strand, within IAI47_02985 at 618.340 kb on + strand, within IAI47_02985 at 618.341 kb on - strand, within IAI47_02985 at 618.341 kb on - strand, within IAI47_02985 at 618.341 kb on - strand, within IAI47_02985 at 618.341 kb on - strand, within IAI47_02985 at 618.344 kb on + strand, within IAI47_02985 at 618.344 kb on + strand, within IAI47_02985 at 618.344 kb on + strand, within IAI47_02985 at 618.344 kb on + strand, within IAI47_02985 at 618.345 kb on - strand, within IAI47_02985 at 618.345 kb on - strand, within IAI47_02985 at 618.345 kb on - strand, within IAI47_02985 at 618.345 kb on - strand, within IAI47_02985 at 618.345 kb on - strand, within IAI47_02985 at 618.345 kb on - strand, within IAI47_02985 at 618.379 kb on + strand, within IAI47_02985 at 618.380 kb on - strand, within IAI47_02985 at 618.380 kb on - strand, within IAI47_02985 at 618.380 kb on - strand, within IAI47_02985 at 618.400 kb on + strand, within IAI47_02985 at 618.400 kb on + strand, within IAI47_02985 at 618.400 kb on + strand, within IAI47_02985 at 618.526 kb on + strand, within IAI47_02985 at 618.526 kb on + strand, within IAI47_02985 at 618.526 kb on + strand, within IAI47_02985 at 618.527 kb on - strand, within IAI47_02985 at 618.527 kb on - strand, within IAI47_02985 at 618.527 kb on - strand, within IAI47_02985 at 618.530 kb on + strand, within IAI47_02985 at 618.530 kb on + strand, within IAI47_02985 at 618.530 kb on + strand, within IAI47_02985 at 618.531 kb on - strand, within IAI47_02985 at 618.625 kb on + strand at 618.625 kb on + strand at 618.626 kb on - strand at 618.686 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.739 kb on + strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.740 kb on - strand at 618.760 kb on + strand at 618.761 kb on - strand at 618.777 kb on - strand at 618.792 kb on + strand at 618.792 kb on + strand at 618.792 kb on + strand at 618.792 kb on + strand at 618.792 kb on + strand at 618.792 kb on + strand at 618.792 kb on + strand at 618.793 kb on - strand at 618.793 kb on - strand at 618.814 kb on + strand at 618.815 kb on - strand at 618.815 kb on - strand at 618.824 kb on + strand at 618.824 kb on + strand at 618.825 kb on - strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.837 kb on + strand at 618.838 kb on - strand at 618.838 kb on - strand at 618.843 kb on - strand at 618.909 kb on + strand at 618.909 kb on + strand at 618.909 kb on + strand at 618.910 kb on - strand at 618.910 kb on - strand at 618.910 kb on - strand at 618.910 kb on - strand at 618.966 kb on + strand at 618.966 kb on + strand at 618.966 kb on + strand at 618.966 kb on + strand at 618.967 kb on - strand at 618.984 kb on + strand at 618.984 kb on + strand at 618.984 kb on + strand at 618.984 kb on + strand at 618.984 kb on + strand at 618.984 kb on + strand at 618.985 kb on - strand at 618.996 kb on + strand at 618.996 kb on + strand at 618.997 kb on - strand at 618.997 kb on - strand at 618.997 kb on - strand at 619.022 kb on - strand at 619.060 kb on + strand at 619.061 kb on - strand at 619.113 kb on + strand at 619.114 kb on - strand at 619.134 kb on + strand at 619.134 kb on + strand at 619.134 kb on + strand at 619.134 kb on + strand at 619.135 kb on - strand at 619.173 kb on - strand at 619.173 kb on - strand at 619.173 kb on - strand at 619.184 kb on + strand at 619.184 kb on + strand at 619.185 kb on - strand at 619.185 kb on - strand at 619.185 kb on - strand at 619.245 kb on - strand at 619.253 kb on + strand at 619.253 kb on + strand at 619.253 kb on + strand at 619.253 kb on + strand at 619.253 kb on + strand at 619.254 kb on - strand at 619.263 kb on + strand at 619.264 kb on - strand at 619.347 kb on - strand at 619.347 kb on - strand at 619.347 kb on - strand at 619.347 kb on - strand at 619.358 kb on + strand at 619.358 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.378 kb on + strand at 619.379 kb on - strand at 619.379 kb on - strand at 619.379 kb on - strand at 619.379 kb on - strand at 619.379 kb on - strand at 619.379 kb on - strand at 619.422 kb on - strand at 619.422 kb on - strand at 619.432 kb on + strand at 619.433 kb on - strand at 619.433 kb on - strand at 619.436 kb on - strand at 619.439 kb on + strand at 619.440 kb on - strand at 619.440 kb on - strand at 619.440 kb on - strand at 619.453 kb on + strand at 619.454 kb on - strand at 619.454 kb on - strand at 619.454 kb on - strand at 619.472 kb on + strand at 619.473 kb on - strand at 619.476 kb on + strand at 619.477 kb on - strand at 619.477 kb on - strand at 619.477 kb on - strand at 619.477 kb on - strand at 619.477 kb on - strand at 619.518 kb on - strand at 619.589 kb on + strand at 619.590 kb on - strand at 619.643 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 616,634 + IAI47_02980 0.33 +0.2 616,634 + IAI47_02980 0.33 -0.8 616,635 - IAI47_02980 0.33 +1.3 616,777 + IAI47_02980 0.43 -0.0 616,777 + IAI47_02980 0.43 +0.5 616,778 - IAI47_02980 0.43 +0.7 616,778 - IAI47_02980 0.43 -1.8 616,778 - IAI47_02980 0.43 +0.8 616,793 + IAI47_02980 0.44 +1.0 616,793 + IAI47_02980 0.44 -0.8 616,912 + IAI47_02980 0.53 +0.8 616,912 + IAI47_02980 0.53 +1.6 616,912 + IAI47_02980 0.53 +1.2 616,913 - IAI47_02980 0.53 +0.5 616,913 - IAI47_02980 0.53 +0.0 616,934 + IAI47_02980 0.54 -1.1 616,934 + IAI47_02980 0.54 -1.0 616,935 - IAI47_02980 0.54 -2.5 616,935 - IAI47_02980 0.54 -1.7 616,985 + IAI47_02980 0.58 +3.7 616,986 - IAI47_02980 0.58 -1.3 616,986 - IAI47_02980 0.58 -1.7 616,994 + IAI47_02980 0.58 +0.3 616,994 + IAI47_02980 0.58 -0.1 616,995 - IAI47_02980 0.58 -0.7 617,067 + IAI47_02980 0.64 +0.5 617,068 - IAI47_02980 0.64 -0.4 617,104 + IAI47_02980 0.66 +2.4 617,104 + IAI47_02980 0.66 +1.8 617,104 + IAI47_02980 0.66 -1.3 617,104 + IAI47_02980 0.66 +2.5 617,105 - IAI47_02980 0.66 -0.4 617,105 - IAI47_02980 0.66 -1.8 617,105 - IAI47_02980 0.66 -0.9 617,105 - IAI47_02980 0.66 -0.6 617,105 - IAI47_02980 0.66 -1.7 617,105 - IAI47_02980 0.66 +1.7 617,105 - IAI47_02980 0.66 -0.9 617,106 + IAI47_02980 0.66 -1.0 617,106 + IAI47_02980 0.66 +0.8 617,106 + IAI47_02980 0.66 -0.1 617,106 + IAI47_02980 0.66 -0.1 617,106 + IAI47_02980 0.66 -0.0 617,106 + IAI47_02980 0.66 +0.2 617,106 + IAI47_02980 0.66 -1.5 617,106 + IAI47_02980 0.66 +0.1 617,107 - IAI47_02980 0.66 -0.5 617,107 - IAI47_02980 0.66 +0.5 617,107 - IAI47_02980 0.66 -0.6 617,107 - IAI47_02980 0.66 -0.5 617,107 - IAI47_02980 0.66 +1.2 617,107 - IAI47_02980 0.66 -0.6 617,107 - IAI47_02980 0.66 +0.5 617,129 + IAI47_02980 0.68 -1.7 617,130 - IAI47_02980 0.68 +0.3 617,130 - IAI47_02980 0.68 +0.0 617,144 + IAI47_02980 0.69 +1.1 617,145 - IAI47_02980 0.69 -1.5 617,145 - IAI47_02980 0.69 -0.3 617,145 - IAI47_02980 0.69 -0.7 617,176 - IAI47_02980 0.71 -1.5 617,176 - IAI47_02980 0.71 -0.3 617,176 - IAI47_02980 0.71 -0.4 617,189 + IAI47_02980 0.72 -3.1 617,190 - IAI47_02980 0.72 -2.4 617,190 - IAI47_02980 0.72 -0.3 617,264 + IAI47_02980 0.77 -1.9 617,340 + IAI47_02980 0.83 +1.9 617,341 - IAI47_02980 0.83 -0.3 617,341 - IAI47_02980 0.83 -2.0 617,403 - IAI47_02980 0.87 +0.4 617,403 - IAI47_02980 0.87 -1.0 617,438 + IAI47_02980 0.90 +1.8 617,439 - IAI47_02980 0.90 +0.1 617,439 - IAI47_02980 0.90 +1.8 617,511 + -0.5 617,512 - -2.7 617,512 - -0.5 617,556 + -0.3 617,556 + -0.3 617,556 + -0.3 617,557 - +1.3 617,557 - +0.6 617,557 - +0.3 617,557 - -0.4 617,557 - -0.9 617,557 - +0.2 617,663 + +0.2 617,663 + -0.4 617,663 + -0.8 617,664 - +0.4 617,721 + IAI47_02985 0.10 -0.4 617,721 + IAI47_02985 0.10 -1.1 617,721 + IAI47_02985 0.10 -1.4 617,722 - IAI47_02985 0.11 -0.5 617,737 + IAI47_02985 0.12 -0.3 617,737 + IAI47_02985 0.12 -1.7 617,738 - IAI47_02985 0.12 +1.1 617,833 + IAI47_02985 0.21 -0.5 617,923 + IAI47_02985 0.29 -0.8 617,924 - IAI47_02985 0.29 -0.9 617,924 - IAI47_02985 0.29 -1.0 617,924 - IAI47_02985 0.29 -2.5 617,924 - IAI47_02985 0.29 -0.8 617,924 - IAI47_02985 0.29 +0.2 617,924 - IAI47_02985 0.29 -0.4 617,926 - IAI47_02985 0.29 +0.5 617,926 - IAI47_02985 0.29 -0.2 617,937 + IAI47_02985 0.30 -0.2 617,937 + IAI47_02985 0.30 -1.6 617,937 + IAI47_02985 0.30 -0.3 617,937 + IAI47_02985 0.30 -1.9 617,943 + IAI47_02985 0.31 -0.1 617,944 - IAI47_02985 0.31 -0.8 617,944 - IAI47_02985 0.31 +0.9 617,944 - IAI47_02985 0.31 +1.0 617,944 - IAI47_02985 0.31 -0.5 617,944 - IAI47_02985 0.31 +0.5 618,032 + IAI47_02985 0.39 -2.1 618,033 - IAI47_02985 0.39 +0.2 618,050 + IAI47_02985 0.40 -0.3 618,051 - IAI47_02985 0.41 -1.3 618,051 - IAI47_02985 0.41 +3.1 618,106 + IAI47_02985 0.46 -0.2 618,106 + IAI47_02985 0.46 -1.9 618,106 + IAI47_02985 0.46 -0.4 618,106 + IAI47_02985 0.46 +0.7 618,107 - IAI47_02985 0.46 +0.8 618,107 - IAI47_02985 0.46 +0.8 618,107 - IAI47_02985 0.46 -0.5 618,107 - IAI47_02985 0.46 +0.8 618,110 + IAI47_02985 0.46 -0.7 618,110 + IAI47_02985 0.46 -1.8 618,110 + IAI47_02985 0.46 -1.0 618,110 + IAI47_02985 0.46 +0.6 618,110 + IAI47_02985 0.46 -0.9 618,111 - IAI47_02985 0.46 -1.4 618,111 - IAI47_02985 0.46 +0.3 618,111 - IAI47_02985 0.46 -1.1 618,111 - IAI47_02985 0.46 -0.2 618,111 - IAI47_02985 0.46 -0.1 618,111 - IAI47_02985 0.46 -1.3 618,111 - IAI47_02985 0.46 -1.1 618,111 - IAI47_02985 0.46 -2.4 618,111 - IAI47_02985 0.46 -0.2 618,111 - IAI47_02985 0.46 +0.6 618,111 - IAI47_02985 0.46 +1.3 618,111 - IAI47_02985 0.46 +0.8 618,111 - IAI47_02985 0.46 -1.1 618,111 - IAI47_02985 0.46 -0.7 618,155 - IAI47_02985 0.50 -1.0 618,155 - IAI47_02985 0.50 -1.0 618,155 - IAI47_02985 0.50 -1.1 618,158 + IAI47_02985 0.50 -1.7 618,158 + IAI47_02985 0.50 +0.7 618,159 - IAI47_02985 0.50 -1.0 618,260 + IAI47_02985 0.60 +0.7 618,260 + IAI47_02985 0.60 -1.1 618,261 - IAI47_02985 0.60 -1.6 618,261 - IAI47_02985 0.60 +0.3 618,329 + IAI47_02985 0.66 -1.7 618,329 + IAI47_02985 0.66 +0.5 618,340 + IAI47_02985 0.67 -1.3 618,340 + IAI47_02985 0.67 +0.4 618,340 + IAI47_02985 0.67 -0.6 618,340 + IAI47_02985 0.67 -0.7 618,341 - IAI47_02985 0.67 +0.4 618,341 - IAI47_02985 0.67 -0.9 618,341 - 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Or see this region's nucleotide sequence