Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_02565 and IAI47_02570 overlap by 4 nucleotides IAI47_02570 and IAI47_02575 overlap by 4 nucleotides IAI47_02575 and IAI47_02580 are separated by 46 nucleotides
IAI47_02565: IAI47_02565 - bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase, at 529,710 to 531,230
_02565
IAI47_02570: IAI47_02570 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE, at 531,227 to 531,703
_02570
IAI47_02575: IAI47_02575 - N-acetylmuramoyl-L-alanine amidase AmiB, at 531,700 to 533,370
_02575
IAI47_02580: IAI47_02580 - DNA mismatch repair endonuclease MutL, at 533,417 to 535,282
_02580
Position (kb) 531
532
533
534 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 530.745 kb on - strand, within IAI47_02565 at 530.780 kb on + strand, within IAI47_02565 at 530.780 kb on + strand, within IAI47_02565 at 530.781 kb on - strand, within IAI47_02565 at 530.822 kb on + strand, within IAI47_02565 at 530.822 kb on + strand, within IAI47_02565 at 530.822 kb on + strand, within IAI47_02565 at 530.822 kb on + strand, within IAI47_02565 at 530.823 kb on - strand, within IAI47_02565 at 530.823 kb on - strand, within IAI47_02565 at 530.823 kb on - strand, within IAI47_02565 at 530.823 kb on - strand, within IAI47_02565 at 530.825 kb on + strand, within IAI47_02565 at 530.825 kb on + strand, within IAI47_02565 at 530.826 kb on - strand, within IAI47_02565 at 530.826 kb on - strand, within IAI47_02565 at 530.826 kb on - strand, within IAI47_02565 at 530.854 kb on + strand, within IAI47_02565 at 530.855 kb on - strand, within IAI47_02565 at 530.893 kb on + strand, within IAI47_02565 at 530.893 kb on + strand, within IAI47_02565 at 530.893 kb on + strand, within IAI47_02565 at 530.893 kb on + strand, within IAI47_02565 at 530.894 kb on - strand, within IAI47_02565 at 530.894 kb on - strand, within IAI47_02565 at 530.894 kb on - strand, within IAI47_02565 at 530.894 kb on - strand, within IAI47_02565 at 530.921 kb on + strand, within IAI47_02565 at 530.922 kb on + strand, within IAI47_02565 at 530.922 kb on + strand, within IAI47_02565 at 530.923 kb on - strand, within IAI47_02565 at 530.923 kb on - strand, within IAI47_02565 at 530.923 kb on - strand, within IAI47_02565 at 530.932 kb on + strand, within IAI47_02565 at 531.026 kb on + strand, within IAI47_02565 at 531.026 kb on + strand, within IAI47_02565 at 531.026 kb on + strand, within IAI47_02565 at 531.027 kb on - strand, within IAI47_02565 at 531.027 kb on - strand, within IAI47_02565 at 531.027 kb on - strand, within IAI47_02565 at 531.050 kb on + strand, within IAI47_02565 at 531.050 kb on + strand, within IAI47_02565 at 531.050 kb on + strand, within IAI47_02565 at 531.161 kb on + strand at 531.161 kb on + strand at 531.178 kb on + strand at 531.178 kb on + strand at 531.179 kb on - strand at 531.218 kb on + strand at 531.218 kb on + strand at 531.218 kb on + strand at 531.218 kb on + strand at 531.218 kb on + strand at 531.694 kb on + strand at 531.694 kb on + strand at 531.728 kb on + strand at 532.057 kb on + strand, within IAI47_02575 at 532.940 kb on + strand, within IAI47_02575 at 532.940 kb on + strand, within IAI47_02575 at 532.941 kb on - strand, within IAI47_02575 at 532.941 kb on - strand, within IAI47_02575 at 532.941 kb on - strand, within IAI47_02575 at 532.998 kb on - strand, within IAI47_02575 at 533.005 kb on - strand, within IAI47_02575 at 533.005 kb on - strand, within IAI47_02575 at 533.021 kb on + strand, within IAI47_02575 at 533.029 kb on + strand, within IAI47_02575 at 533.029 kb on + strand, within IAI47_02575 at 533.029 kb on + strand, within IAI47_02575 at 533.029 kb on + strand, within IAI47_02575 at 533.029 kb on + strand, within IAI47_02575 at 533.030 kb on - strand, within IAI47_02575 at 533.030 kb on - strand, within IAI47_02575 at 533.053 kb on - strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.095 kb on + strand, within IAI47_02575 at 533.096 kb on - strand, within IAI47_02575 at 533.096 kb on - strand, within IAI47_02575 at 533.096 kb on - strand, within IAI47_02575 at 533.168 kb on + strand, within IAI47_02575 at 533.169 kb on - strand, within IAI47_02575 at 533.225 kb on + strand at 533.225 kb on + strand at 533.225 kb on + strand at 533.225 kb on + strand at 533.226 kb on - strand at 533.226 kb on - strand at 533.226 kb on - strand at 533.226 kb on - strand at 533.228 kb on + strand at 533.228 kb on + strand at 533.228 kb on + strand at 533.229 kb on - strand at 533.229 kb on - strand at 533.235 kb on + strand at 533.278 kb on + strand at 533.279 kb on - strand at 533.279 kb on - strand at 533.279 kb on - strand at 533.310 kb on + strand at 533.310 kb on + strand at 533.310 kb on + strand at 533.311 kb on - strand at 533.311 kb on - strand at 533.311 kb on - strand at 533.311 kb on - strand at 533.311 kb on - strand at 533.311 kb on - strand at 533.313 kb on + strand at 533.313 kb on + strand at 533.313 kb on + strand at 533.313 kb on + strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.314 kb on - strand at 533.404 kb on + strand at 533.551 kb on + strand at 533.608 kb on + strand, within IAI47_02580 at 533.644 kb on + strand, within IAI47_02580 at 533.644 kb on + strand, within IAI47_02580 at 533.655 kb on + strand, within IAI47_02580 at 533.683 kb on + strand, within IAI47_02580 at 533.683 kb on + strand, within IAI47_02580 at 533.684 kb on - strand, within IAI47_02580 at 533.684 kb on - strand, within IAI47_02580 at 533.684 kb on - strand, within IAI47_02580 at 533.722 kb on + strand, within IAI47_02580 at 533.722 kb on + strand, within IAI47_02580 at 533.723 kb on - strand, within IAI47_02580 at 533.723 kb on - strand, within IAI47_02580 at 533.723 kb on - strand, within IAI47_02580 at 533.723 kb on - strand, within IAI47_02580 at 533.723 kb on - strand, within IAI47_02580 at 533.723 kb on - strand, within IAI47_02580 at 533.745 kb on + strand, within IAI47_02580 at 533.746 kb on - strand, within IAI47_02580 at 533.746 kb on - strand, within IAI47_02580 at 533.755 kb on + strand, within IAI47_02580 at 533.756 kb on - strand, within IAI47_02580 at 533.786 kb on + strand, within IAI47_02580 at 533.786 kb on + strand, within IAI47_02580 at 533.786 kb on + strand, within IAI47_02580 at 533.787 kb on - strand, within IAI47_02580 at 533.787 kb on - strand, within IAI47_02580 at 533.787 kb on - strand, within IAI47_02580 at 533.818 kb on + strand, within IAI47_02580 at 533.818 kb on + strand, within IAI47_02580 at 533.818 kb on + strand, within IAI47_02580 at 533.871 kb on - strand, within IAI47_02580 at 533.872 kb on + strand, within IAI47_02580 at 533.873 kb on - strand, within IAI47_02580 at 533.926 kb on + strand, within IAI47_02580 at 533.926 kb on + strand, within IAI47_02580 at 533.927 kb on - strand, within IAI47_02580 at 533.948 kb on - strand, within IAI47_02580 at 533.948 kb on - strand, within IAI47_02580 at 533.948 kb on - strand, within IAI47_02580 at 533.977 kb on - strand, within IAI47_02580 at 533.977 kb on - strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.982 kb on + strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand at 533.983 kb on - strand, within IAI47_02580 at 533.983 kb on - strand, within IAI47_02580 at 533.995 kb on + strand, within IAI47_02580 at 533.996 kb on - strand, within IAI47_02580 at 534.011 kb on + strand, within IAI47_02580 at 534.076 kb on + strand, within IAI47_02580 at 534.076 kb on + strand, within IAI47_02580 at 534.077 kb on - strand, within IAI47_02580 at 534.077 kb on - strand, within IAI47_02580 at 534.077 kb on - strand, within IAI47_02580 at 534.077 kb on - strand, within IAI47_02580 at 534.176 kb on + strand, within IAI47_02580 at 534.176 kb on + strand, within IAI47_02580 at 534.176 kb on + strand, within IAI47_02580 at 534.176 kb on + strand, within IAI47_02580 at 534.177 kb on - strand, within IAI47_02580 at 534.177 kb on - strand, within IAI47_02580 at 534.177 kb on - strand, within IAI47_02580 at 534.177 kb on - strand, within IAI47_02580 at 534.182 kb on + strand, within IAI47_02580 at 534.182 kb on + strand, within IAI47_02580 at 534.182 kb on + strand, within IAI47_02580 at 534.182 kb on + strand, within IAI47_02580 at 534.182 kb on + strand, within IAI47_02580 at 534.182 kb on + strand, within IAI47_02580 at 534.183 kb on - strand, within IAI47_02580 at 534.183 kb on - strand, within IAI47_02580 at 534.183 kb on - strand, within IAI47_02580 at 534.227 kb on - strand, within IAI47_02580 at 534.275 kb on + strand, within IAI47_02580 at 534.275 kb on + strand, within IAI47_02580 at 534.275 kb on + strand, within IAI47_02580 at 534.276 kb on - strand, within IAI47_02580 at 534.285 kb on - strand, within IAI47_02580 at 534.285 kb on - strand, within IAI47_02580 at 534.342 kb on + strand, within IAI47_02580 at 534.343 kb on - strand, within IAI47_02580 at 534.343 kb on - strand, within IAI47_02580 at 534.343 kb on - strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.366 kb on + strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580 at 534.367 kb on - strand, within IAI47_02580
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 530,745 - IAI47_02565 0.68 -0.8 530,780 + IAI47_02565 0.70 -2.0 530,780 + IAI47_02565 0.70 -0.3 530,781 - IAI47_02565 0.70 -0.3 530,822 + IAI47_02565 0.73 +1.2 530,822 + IAI47_02565 0.73 +0.5 530,822 + IAI47_02565 0.73 -0.9 530,822 + IAI47_02565 0.73 +0.9 530,823 - IAI47_02565 0.73 -0.9 530,823 - IAI47_02565 0.73 -1.6 530,823 - IAI47_02565 0.73 -1.6 530,823 - IAI47_02565 0.73 +1.1 530,825 + IAI47_02565 0.73 +0.5 530,825 + IAI47_02565 0.73 -1.4 530,826 - IAI47_02565 0.73 +0.5 530,826 - IAI47_02565 0.73 +0.5 530,826 - IAI47_02565 0.73 +0.1 530,854 + IAI47_02565 0.75 -0.7 530,855 - IAI47_02565 0.75 -1.3 530,893 + IAI47_02565 0.78 -0.9 530,893 + IAI47_02565 0.78 +2.3 530,893 + IAI47_02565 0.78 -0.3 530,893 + IAI47_02565 0.78 +1.8 530,894 - IAI47_02565 0.78 -0.7 530,894 - IAI47_02565 0.78 -2.5 530,894 - IAI47_02565 0.78 -0.2 530,894 - IAI47_02565 0.78 -2.4 530,921 + IAI47_02565 0.80 +0.7 530,922 + IAI47_02565 0.80 +2.3 530,922 + IAI47_02565 0.80 -0.0 530,923 - IAI47_02565 0.80 -0.4 530,923 - IAI47_02565 0.80 +0.3 530,923 - IAI47_02565 0.80 -0.5 530,932 + IAI47_02565 0.80 -0.2 531,026 + IAI47_02565 0.87 -0.4 531,026 + IAI47_02565 0.87 -0.8 531,026 + IAI47_02565 0.87 +3.1 531,027 - IAI47_02565 0.87 -0.5 531,027 - IAI47_02565 0.87 +0.7 531,027 - IAI47_02565 0.87 -1.2 531,050 + IAI47_02565 0.88 -1.0 531,050 + IAI47_02565 0.88 +1.4 531,050 + IAI47_02565 0.88 -0.2 531,161 + -1.1 531,161 + +0.3 531,178 + +2.5 531,178 + -0.4 531,179 - +1.4 531,218 + -0.1 531,218 + +1.6 531,218 + -0.8 531,218 + +1.3 531,218 + -0.3 531,694 + -0.1 531,694 + +1.3 531,728 + -1.3 532,057 + IAI47_02575 0.21 -1.1 532,940 + IAI47_02575 0.74 +1.4 532,940 + IAI47_02575 0.74 +0.8 532,941 - IAI47_02575 0.74 -0.7 532,941 - IAI47_02575 0.74 -0.3 532,941 - IAI47_02575 0.74 +2.0 532,998 - IAI47_02575 0.78 +0.2 533,005 - IAI47_02575 0.78 +1.1 533,005 - IAI47_02575 0.78 +0.2 533,021 + IAI47_02575 0.79 +1.3 533,029 + IAI47_02575 0.80 -0.4 533,029 + IAI47_02575 0.80 +0.3 533,029 + IAI47_02575 0.80 +1.4 533,029 + IAI47_02575 0.80 -0.2 533,029 + IAI47_02575 0.80 +0.4 533,030 - IAI47_02575 0.80 +0.0 533,030 - IAI47_02575 0.80 +1.3 533,053 - IAI47_02575 0.81 -0.3 533,095 + IAI47_02575 0.83 -0.7 533,095 + IAI47_02575 0.83 +0.4 533,095 + IAI47_02575 0.83 +1.4 533,095 + IAI47_02575 0.83 -0.2 533,095 + IAI47_02575 0.83 +0.6 533,095 + IAI47_02575 0.83 +0.5 533,095 + IAI47_02575 0.83 -0.1 533,096 - IAI47_02575 0.84 -1.8 533,096 - IAI47_02575 0.84 +1.6 533,096 - IAI47_02575 0.84 -0.2 533,168 + IAI47_02575 0.88 +0.2 533,169 - IAI47_02575 0.88 -3.4 533,225 + +0.2 533,225 + +3.8 533,225 + -0.2 533,225 + +2.6 533,226 - -0.3 533,226 - -1.3 533,226 - -1.8 533,226 - -0.4 533,228 + +0.4 533,228 + +0.5 533,228 + +2.3 533,229 - -0.6 533,229 - -0.8 533,235 + -1.4 533,278 + -0.2 533,279 - -1.5 533,279 - -0.2 533,279 - +1.0 533,310 + -0.6 533,310 + -1.3 533,310 + -0.7 533,311 - -1.8 533,311 - -1.0 533,311 - -0.3 533,311 - +0.3 533,311 - -0.7 533,311 - -0.9 533,313 + -2.0 533,313 + +0.2 533,313 + +1.3 533,313 + -1.1 533,314 - -1.7 533,314 - -0.7 533,314 - -0.4 533,314 - +0.3 533,314 - -0.7 533,314 - -1.3 533,314 - +2.0 533,314 - +0.2 533,314 - -1.0 533,404 + +1.3 533,551 + +0.7 533,608 + IAI47_02580 0.10 +0.0 533,644 + IAI47_02580 0.12 -1.4 533,644 + IAI47_02580 0.12 +1.3 533,655 + IAI47_02580 0.13 +0.3 533,683 + IAI47_02580 0.14 -0.6 533,683 + IAI47_02580 0.14 +0.1 533,684 - IAI47_02580 0.14 +0.7 533,684 - IAI47_02580 0.14 -2.8 533,684 - IAI47_02580 0.14 -0.4 533,722 + IAI47_02580 0.16 -1.6 533,722 + IAI47_02580 0.16 -1.6 533,723 - IAI47_02580 0.16 -1.5 533,723 - IAI47_02580 0.16 -0.3 533,723 - IAI47_02580 0.16 -0.4 533,723 - IAI47_02580 0.16 -0.4 533,723 - IAI47_02580 0.16 -1.7 533,723 - IAI47_02580 0.16 -0.3 533,745 + IAI47_02580 0.18 +0.6 533,746 - IAI47_02580 0.18 +0.9 533,746 - IAI47_02580 0.18 -0.5 533,755 + IAI47_02580 0.18 +1.4 533,756 - IAI47_02580 0.18 +0.8 533,786 + IAI47_02580 0.20 -2.3 533,786 + IAI47_02580 0.20 -1.1 533,786 + IAI47_02580 0.20 -2.5 533,787 - IAI47_02580 0.20 +0.0 533,787 - IAI47_02580 0.20 -0.6 533,787 - IAI47_02580 0.20 -1.1 533,818 + IAI47_02580 0.21 -0.8 533,818 + IAI47_02580 0.21 +1.3 533,818 + IAI47_02580 0.21 -1.4 533,871 - IAI47_02580 0.24 -0.1 533,872 + IAI47_02580 0.24 -1.1 533,873 - IAI47_02580 0.24 +0.4 533,926 + IAI47_02580 0.27 +1.9 533,926 + IAI47_02580 0.27 +0.3 533,927 - IAI47_02580 0.27 +2.2 533,948 - IAI47_02580 0.28 -0.9 533,948 - IAI47_02580 0.28 +1.9 533,948 - IAI47_02580 0.28 -2.3 533,977 - IAI47_02580 0.30 -2.4 533,977 - IAI47_02580 0.30 +0.5 533,982 + IAI47_02580 0.30 +2.8 533,982 + IAI47_02580 0.30 -0.6 533,982 + IAI47_02580 0.30 -0.6 533,982 + IAI47_02580 0.30 -1.4 533,982 + IAI47_02580 0.30 -1.1 533,982 + IAI47_02580 0.30 +0.6 533,982 + IAI47_02580 0.30 +1.0 533,983 - IAI47_02580 0.30 +0.4 533,983 - IAI47_02580 0.30 -0.7 533,983 - IAI47_02580 0.30 -0.6 533,983 - IAI47_02580 0.30 -0.9 533,983 - IAI47_02580 0.30 +0.3 533,983 - IAI47_02580 0.30 -0.1 533,983 - IAI47_02580 0.30 +0.0 533,983 - IAI47_02580 0.30 -0.9 533,983 - IAI47_02580 0.30 -0.5 533,983 - +2.8 533,983 - IAI47_02580 0.30 -1.2 533,983 - IAI47_02580 0.30 -2.1 533,995 + IAI47_02580 0.31 +1.0 533,996 - IAI47_02580 0.31 -0.5 534,011 + IAI47_02580 0.32 -1.4 534,076 + IAI47_02580 0.35 -0.4 534,076 + IAI47_02580 0.35 -1.1 534,077 - IAI47_02580 0.35 +1.5 534,077 - IAI47_02580 0.35 -0.7 534,077 - IAI47_02580 0.35 -1.8 534,077 - IAI47_02580 0.35 +0.3 534,176 + IAI47_02580 0.41 -1.3 534,176 + IAI47_02580 0.41 -0.4 534,176 + IAI47_02580 0.41 -0.7 534,176 + IAI47_02580 0.41 +2.7 534,177 - IAI47_02580 0.41 -1.2 534,177 - IAI47_02580 0.41 -0.6 534,177 - IAI47_02580 0.41 -0.7 534,177 - IAI47_02580 0.41 -0.3 534,182 + IAI47_02580 0.41 +2.8 534,182 + IAI47_02580 0.41 -0.2 534,182 + IAI47_02580 0.41 +0.4 534,182 + IAI47_02580 0.41 -1.1 534,182 + IAI47_02580 0.41 -1.0 534,182 + IAI47_02580 0.41 +0.4 534,183 - IAI47_02580 0.41 -0.5 534,183 - IAI47_02580 0.41 +2.5 534,183 - IAI47_02580 0.41 +0.4 534,227 - IAI47_02580 0.43 -2.3 534,275 + IAI47_02580 0.46 -1.8 534,275 + IAI47_02580 0.46 +0.6 534,275 + IAI47_02580 0.46 -1.1 534,276 - IAI47_02580 0.46 +0.7 534,285 - IAI47_02580 0.47 -0.4 534,285 - IAI47_02580 0.47 -1.4 534,342 + IAI47_02580 0.50 -0.5 534,343 - IAI47_02580 0.50 +0.6 534,343 - IAI47_02580 0.50 +2.5 534,343 - IAI47_02580 0.50 -0.5 534,366 + IAI47_02580 0.51 -0.8 534,366 + IAI47_02580 0.51 -1.5 534,366 + IAI47_02580 0.51 +1.0 534,366 + IAI47_02580 0.51 -1.4 534,366 + IAI47_02580 0.51 +2.6 534,367 - IAI47_02580 0.51 -1.3 534,367 - IAI47_02580 0.51 -0.1 534,367 - IAI47_02580 0.51 +0.4 534,367 - IAI47_02580 0.51 +1.3 534,367 - IAI47_02580 0.51 -0.4
Or see this region's nucleotide sequence