Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01330 and IAI47_01335 overlap by 4 nucleotides IAI47_01335 and IAI47_01340 are separated by 6 nucleotides IAI47_01340 and IAI47_01345 are separated by 430 nucleotides
IAI47_01330: IAI47_01330 - ribonuclease Z, at 287,187 to 288,098
_01330
IAI47_01335: IAI47_01335 - CPBP family intramembrane metalloprotease, at 288,095 to 288,913
_01335
IAI47_01340: IAI47_01340 - 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ, at 288,920 to 289,762
_01340
IAI47_01345: IAI47_01345 - oligopeptidase A, at 290,193 to 292,235
_01345
Position (kb) 288
289
290 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 288.004 kb on - strand, within IAI47_01330 at 288.086 kb on - strand at 288.086 kb on - strand at 288.086 kb on - strand at 288.096 kb on + strand at 288.096 kb on + strand at 288.097 kb on - strand at 288.114 kb on + strand at 288.114 kb on + strand at 288.115 kb on - strand at 288.118 kb on + strand at 288.118 kb on + strand at 288.118 kb on + strand at 288.118 kb on + strand at 288.119 kb on - strand at 288.119 kb on - strand at 288.119 kb on - strand at 288.142 kb on + strand at 288.204 kb on + strand, within IAI47_01335 at 288.205 kb on - strand, within IAI47_01335 at 288.230 kb on + strand, within IAI47_01335 at 288.230 kb on + strand, within IAI47_01335 at 288.256 kb on + strand, within IAI47_01335 at 288.256 kb on + strand, within IAI47_01335 at 288.257 kb on - strand, within IAI47_01335 at 288.257 kb on - strand, within IAI47_01335 at 288.337 kb on - strand, within IAI47_01335 at 288.391 kb on + strand, within IAI47_01335 at 288.454 kb on + strand, within IAI47_01335 at 288.454 kb on + strand, within IAI47_01335 at 288.455 kb on - strand, within IAI47_01335 at 288.455 kb on - strand, within IAI47_01335 at 288.580 kb on + strand, within IAI47_01335 at 288.656 kb on - strand, within IAI47_01335 at 288.704 kb on + strand, within IAI47_01335 at 288.819 kb on + strand, within IAI47_01335 at 288.819 kb on + strand, within IAI47_01335 at 288.819 kb on + strand, within IAI47_01335 at 288.846 kb on - strand at 288.854 kb on + strand at 288.921 kb on + strand at 288.922 kb on - strand at 288.941 kb on + strand at 288.942 kb on - strand at 288.948 kb on + strand at 288.949 kb on - strand at 288.949 kb on - strand at 289.000 kb on + strand at 289.000 kb on + strand at 289.000 kb on + strand at 289.001 kb on - strand at 289.001 kb on - strand at 289.001 kb on - strand at 289.034 kb on - strand, within IAI47_01340 at 289.034 kb on - strand, within IAI47_01340 at 289.108 kb on + strand, within IAI47_01340 at 289.109 kb on - strand, within IAI47_01340 at 289.163 kb on + strand, within IAI47_01340 at 289.164 kb on - strand, within IAI47_01340 at 289.176 kb on + strand, within IAI47_01340 at 289.177 kb on - strand, within IAI47_01340 at 289.210 kb on + strand, within IAI47_01340 at 289.210 kb on + strand, within IAI47_01340 at 289.211 kb on - strand, within IAI47_01340 at 289.211 kb on - strand, within IAI47_01340 at 289.245 kb on + strand, within IAI47_01340 at 289.245 kb on + strand, within IAI47_01340 at 289.263 kb on + strand, within IAI47_01340 at 289.360 kb on + strand, within IAI47_01340 at 289.360 kb on + strand, within IAI47_01340 at 289.393 kb on - strand, within IAI47_01340 at 289.473 kb on + strand, within IAI47_01340 at 289.473 kb on + strand, within IAI47_01340 at 289.473 kb on + strand, within IAI47_01340 at 289.473 kb on + strand, within IAI47_01340 at 289.474 kb on - strand, within IAI47_01340 at 289.474 kb on - strand, within IAI47_01340 at 289.474 kb on - strand, within IAI47_01340 at 289.474 kb on - strand, within IAI47_01340 at 289.474 kb on - strand, within IAI47_01340 at 289.476 kb on + strand, within IAI47_01340 at 289.476 kb on + strand, within IAI47_01340 at 289.477 kb on - strand, within IAI47_01340 at 289.477 kb on - strand, within IAI47_01340 at 289.477 kb on - strand, within IAI47_01340 at 289.521 kb on + strand, within IAI47_01340 at 289.522 kb on - strand, within IAI47_01340 at 289.561 kb on + strand, within IAI47_01340 at 289.561 kb on + strand, within IAI47_01340 at 289.562 kb on - strand, within IAI47_01340 at 289.562 kb on - strand, within IAI47_01340 at 289.562 kb on - strand, within IAI47_01340 at 289.562 kb on - strand, within IAI47_01340 at 289.581 kb on + strand, within IAI47_01340 at 289.581 kb on + strand, within IAI47_01340 at 289.581 kb on + strand, within IAI47_01340 at 289.582 kb on - strand, within IAI47_01340 at 289.582 kb on - strand, within IAI47_01340 at 289.650 kb on + strand, within IAI47_01340 at 289.753 kb on - strand at 289.781 kb on + strand at 289.781 kb on + strand at 289.784 kb on - strand at 289.791 kb on + strand at 289.812 kb on - strand at 289.820 kb on + strand at 289.820 kb on + strand at 289.820 kb on + strand at 289.820 kb on + strand at 289.821 kb on - strand at 289.821 kb on - strand at 289.821 kb on - strand at 289.821 kb on - strand at 289.821 kb on - strand at 289.832 kb on + strand at 289.832 kb on + strand at 289.832 kb on + strand at 289.832 kb on + strand at 289.832 kb on + strand at 289.832 kb on + strand at 289.832 kb on + strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.833 kb on - strand at 289.835 kb on + strand at 289.890 kb on + strand at 289.890 kb on + strand at 289.890 kb on + strand at 289.890 kb on + strand at 289.890 kb on + strand at 289.891 kb on - strand at 289.892 kb on + strand at 289.892 kb on + strand at 289.892 kb on + strand at 289.893 kb on - strand at 289.929 kb on - strand at 290.119 kb on + strand at 290.119 kb on + strand at 290.124 kb on + strand at 290.124 kb on + strand at 290.124 kb on + strand at 290.124 kb on + strand at 290.124 kb on + strand at 290.124 kb on + strand at 290.125 kb on - strand at 290.125 kb on - strand at 290.136 kb on + strand at 290.136 kb on + strand at 290.136 kb on + strand at 290.137 kb on - strand at 290.140 kb on + strand at 290.140 kb on + strand at 290.140 kb on + strand at 290.140 kb on + strand at 290.140 kb on + strand at 290.140 kb on + strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.141 kb on - strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.171 kb on + strand at 290.172 kb on - strand at 290.172 kb on - strand at 290.172 kb on - strand at 290.172 kb on - strand at 290.172 kb on - strand at 290.179 kb on + strand at 290.179 kb on + strand at 290.179 kb on + strand at 290.180 kb on - strand at 290.193 kb on - strand at 290.193 kb on - strand at 290.219 kb on + strand at 290.220 kb on - strand at 290.220 kb on - strand at 290.220 kb on - strand at 290.331 kb on + strand at 290.331 kb on + strand at 290.331 kb on + strand at 290.332 kb on - strand at 290.332 kb on - strand at 290.333 kb on + strand at 290.333 kb on + strand at 290.333 kb on + strand at 290.333 kb on + strand at 290.333 kb on + strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.334 kb on - strand at 290.342 kb on + strand at 290.342 kb on + strand at 290.342 kb on + strand at 290.412 kb on - strand, within IAI47_01345 at 290.448 kb on + strand, within IAI47_01345 at 290.448 kb on + strand, within IAI47_01345 at 290.448 kb on + strand, within IAI47_01345 at 290.448 kb on + strand, within IAI47_01345 at 290.449 kb on - strand, within IAI47_01345 at 290.449 kb on - strand, within IAI47_01345 at 290.449 kb on - strand, within IAI47_01345 at 290.478 kb on + strand, within IAI47_01345 at 290.478 kb on + strand, within IAI47_01345 at 290.478 kb on + strand, within IAI47_01345 at 290.479 kb on - strand, within IAI47_01345 at 290.511 kb on + strand, within IAI47_01345 at 290.511 kb on + strand, within IAI47_01345 at 290.511 kb on + strand, within IAI47_01345 at 290.512 kb on - strand, within IAI47_01345 at 290.547 kb on + strand, within IAI47_01345 at 290.547 kb on + strand, within IAI47_01345 at 290.646 kb on + strand, within IAI47_01345 at 290.688 kb on + strand, within IAI47_01345 at 290.688 kb on + strand, within IAI47_01345 at 290.688 kb on + strand, within IAI47_01345 at 290.689 kb on - strand, within IAI47_01345
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 288,004 - IAI47_01330 0.90 -1.7 288,086 - +0.4 288,086 - -0.0 288,086 - +0.7 288,096 + -0.3 288,096 + +0.6 288,097 - -0.9 288,114 + -0.7 288,114 + -0.6 288,115 - -0.7 288,118 + -2.0 288,118 + -0.5 288,118 + -0.5 288,118 + -1.7 288,119 - +0.7 288,119 - -0.2 288,119 - +0.1 288,142 + +0.2 288,204 + IAI47_01335 0.13 -1.7 288,205 - IAI47_01335 0.13 +1.5 288,230 + IAI47_01335 0.16 -0.1 288,230 + IAI47_01335 0.16 -0.7 288,256 + IAI47_01335 0.20 -0.7 288,256 + IAI47_01335 0.20 +0.4 288,257 - IAI47_01335 0.20 +0.7 288,257 - IAI47_01335 0.20 -0.6 288,337 - IAI47_01335 0.30 -1.3 288,391 + IAI47_01335 0.36 +1.2 288,454 + IAI47_01335 0.44 -0.1 288,454 + IAI47_01335 0.44 -0.2 288,455 - IAI47_01335 0.44 -2.1 288,455 - IAI47_01335 0.44 -0.8 288,580 + IAI47_01335 0.59 -1.0 288,656 - IAI47_01335 0.68 +0.3 288,704 + IAI47_01335 0.74 -0.1 288,819 + IAI47_01335 0.88 -0.8 288,819 + IAI47_01335 0.88 -2.4 288,819 + IAI47_01335 0.88 +0.1 288,846 - -0.2 288,854 + +0.2 288,921 + -0.9 288,922 - +1.3 288,941 + +0.7 288,942 - -0.2 288,948 + -1.7 288,949 - -1.3 288,949 - -0.8 289,000 + -0.7 289,000 + -2.4 289,000 + +0.1 289,001 - -0.3 289,001 - +3.7 289,001 - +0.5 289,034 - IAI47_01340 0.14 -0.2 289,034 - IAI47_01340 0.14 -1.5 289,108 + IAI47_01340 0.22 -1.4 289,109 - IAI47_01340 0.22 +0.4 289,163 + IAI47_01340 0.29 -1.2 289,164 - IAI47_01340 0.29 +0.5 289,176 + IAI47_01340 0.30 +1.2 289,177 - IAI47_01340 0.30 +0.0 289,210 + IAI47_01340 0.34 -1.0 289,210 + IAI47_01340 0.34 -1.2 289,211 - IAI47_01340 0.35 -0.0 289,211 - IAI47_01340 0.35 -0.5 289,245 + IAI47_01340 0.39 -0.9 289,245 + IAI47_01340 0.39 +1.3 289,263 + IAI47_01340 0.41 -1.1 289,360 + IAI47_01340 0.52 -2.0 289,360 + IAI47_01340 0.52 -0.1 289,393 - IAI47_01340 0.56 -0.7 289,473 + IAI47_01340 0.66 -0.0 289,473 + IAI47_01340 0.66 +0.2 289,473 + IAI47_01340 0.66 -2.3 289,473 + IAI47_01340 0.66 +1.2 289,474 - IAI47_01340 0.66 +2.3 289,474 - IAI47_01340 0.66 +0.6 289,474 - IAI47_01340 0.66 -1.2 289,474 - IAI47_01340 0.66 +0.5 289,474 - IAI47_01340 0.66 +0.6 289,476 + IAI47_01340 0.66 -0.7 289,476 + IAI47_01340 0.66 -0.2 289,477 - IAI47_01340 0.66 +2.3 289,477 - IAI47_01340 0.66 -0.1 289,477 - IAI47_01340 0.66 +2.2 289,521 + IAI47_01340 0.71 +1.3 289,522 - IAI47_01340 0.71 -0.8 289,561 + IAI47_01340 0.76 +0.3 289,561 + IAI47_01340 0.76 -0.6 289,562 - IAI47_01340 0.76 +0.0 289,562 - IAI47_01340 0.76 -1.4 289,562 - IAI47_01340 0.76 -2.1 289,562 - IAI47_01340 0.76 -1.3 289,581 + IAI47_01340 0.78 -0.4 289,581 + IAI47_01340 0.78 -0.4 289,581 + IAI47_01340 0.78 -0.6 289,582 - IAI47_01340 0.79 -1.0 289,582 - IAI47_01340 0.79 -1.2 289,650 + IAI47_01340 0.87 -2.0 289,753 - -0.5 289,781 + -0.6 289,781 + -0.6 289,784 - -0.3 289,791 + -0.4 289,812 - -0.0 289,820 + -1.1 289,820 + -1.1 289,820 + -1.0 289,820 + +0.2 289,821 - -0.1 289,821 - +0.2 289,821 - +0.1 289,821 - +0.5 289,821 - +1.1 289,832 + +0.1 289,832 + +1.2 289,832 + -0.4 289,832 + +2.3 289,832 + -0.2 289,832 + -0.9 289,832 + -1.6 289,833 - +0.4 289,833 - +1.9 289,833 - +1.3 289,833 - -0.7 289,833 - +0.0 289,833 - -1.6 289,833 - +0.8 289,833 - +0.6 289,833 - +0.4 289,835 + -0.2 289,890 + -2.0 289,890 + +0.3 289,890 + -1.4 289,890 + +0.3 289,890 + -0.5 289,891 - -0.8 289,892 + +1.7 289,892 + -0.2 289,892 + -0.4 289,893 - -0.1 289,929 - -0.7 290,119 + -1.4 290,119 + +2.0 290,124 + -1.0 290,124 + -1.0 290,124 + +0.4 290,124 + +0.1 290,124 + -1.5 290,124 + -0.8 290,125 - -2.7 290,125 - -2.5 290,136 + -1.1 290,136 + -0.9 290,136 + -2.3 290,137 - -3.0 290,140 + -0.5 290,140 + -0.2 290,140 + +1.0 290,140 + -2.9 290,140 + +0.9 290,140 + -0.8 290,141 - -2.1 290,141 - -0.7 290,141 - -2.7 290,141 - -3.0 290,171 + -2.6 290,171 + -0.3 290,171 + +1.1 290,171 + -0.3 290,171 + -1.9 290,171 + +2.7 290,171 + +0.4 290,171 + +1.1 290,171 + +0.0 290,171 + -1.5 290,171 + -0.8 290,172 - -1.6 290,172 - -0.9 290,172 - -1.9 290,172 - -1.2 290,172 - -0.7 290,179 + +0.9 290,179 + +0.5 290,179 + -0.7 290,180 - -0.8 290,193 - -1.2 290,193 - -3.0 290,219 + +1.5 290,220 - -0.1 290,220 - -2.0 290,220 - -2.3 290,331 + -0.2 290,331 + -1.2 290,331 + -1.4 290,332 - -2.8 290,332 - -0.5 290,333 + -1.0 290,333 + -1.4 290,333 + -0.2 290,333 + -1.9 290,333 + -1.5 290,334 - -1.7 290,334 - -0.7 290,334 - -3.0 290,334 - +1.4 290,334 - -0.7 290,334 - -3.4 290,334 - -2.0 290,334 - +0.2 290,342 + -1.4 290,342 + -0.3 290,342 + -0.5 290,412 - IAI47_01345 0.11 -3.1 290,448 + IAI47_01345 0.12 -1.8 290,448 + IAI47_01345 0.12 +2.4 290,448 + IAI47_01345 0.12 -2.2 290,448 + IAI47_01345 0.12 -1.4 290,449 - IAI47_01345 0.13 -3.4 290,449 - IAI47_01345 0.13 -2.9 290,449 - IAI47_01345 0.13 -0.3 290,478 + IAI47_01345 0.14 +1.4 290,478 + IAI47_01345 0.14 -2.0 290,478 + IAI47_01345 0.14 -0.3 290,479 - IAI47_01345 0.14 -1.7 290,511 + IAI47_01345 0.16 +0.2 290,511 + IAI47_01345 0.16 +0.7 290,511 + IAI47_01345 0.16 -1.6 290,512 - IAI47_01345 0.16 -1.3 290,547 + IAI47_01345 0.17 -2.8 290,547 + IAI47_01345 0.17 -1.5 290,646 + IAI47_01345 0.22 -2.4 290,688 + IAI47_01345 0.24 -1.5 290,688 + IAI47_01345 0.24 -1.7 290,688 + IAI47_01345 0.24 -0.4 290,689 - IAI47_01345 0.24 -1.7
Or see this region's nucleotide sequence