Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27550 and BWI76_RS27555 overlap by 20 nucleotides BWI76_RS27555 and BWI76_RS27560 are separated by 50 nucleotides BWI76_RS27560 and BWI76_RS27565 are separated by 80 nucleotides BWI76_RS27565 and BWI76_RS27570 are separated by 11 nucleotides
BWI76_RS27550: BWI76_RS27550 - hypothetical protein, at 5,719,163 to 5,719,837
_RS27550
BWI76_RS27555: BWI76_RS27555 - putative sugar kinase/transcriptional regulator, ATPase domain protein, at 5,719,818 to 5,720,750
_RS27555
BWI76_RS27560: BWI76_RS27560 - hypothetical protein, at 5,720,801 to 5,721,661
_RS27560
BWI76_RS27565: BWI76_RS27565 - sugar aldolase, at 5,721,742 to 5,722,593
_RS27565
BWI76_RS27570: BWI76_RS27570 - phosphotransferase system, fructose-specific IIC component, at 5,722,605 to 5,723,696
_RS27570
Position (kb) 5720
5721
5722 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5719.992 kb on - strand, within BWI76_RS27555 at 5720.129 kb on + strand, within BWI76_RS27555 at 5720.144 kb on + strand, within BWI76_RS27555 at 5720.152 kb on - strand, within BWI76_RS27555 at 5720.164 kb on - strand, within BWI76_RS27555 at 5720.240 kb on + strand, within BWI76_RS27555 at 5720.245 kb on + strand at 5720.245 kb on + strand, within BWI76_RS27555 at 5720.253 kb on - strand, within BWI76_RS27555 at 5720.253 kb on - strand, within BWI76_RS27555 at 5720.260 kb on + strand, within BWI76_RS27555 at 5720.278 kb on + strand, within BWI76_RS27555 at 5720.335 kb on + strand, within BWI76_RS27555 at 5720.511 kb on - strand, within BWI76_RS27555 at 5720.548 kb on - strand, within BWI76_RS27555 at 5720.548 kb on - strand, within BWI76_RS27555 at 5720.607 kb on + strand, within BWI76_RS27555 at 5720.613 kb on + strand, within BWI76_RS27555 at 5720.700 kb on + strand at 5720.755 kb on + strand at 5720.803 kb on + strand at 5720.804 kb on + strand at 5720.807 kb on - strand at 5720.811 kb on - strand at 5720.899 kb on + strand, within BWI76_RS27560 at 5721.270 kb on + strand, within BWI76_RS27560 at 5721.325 kb on - strand, within BWI76_RS27560 at 5721.371 kb on + strand, within BWI76_RS27560 at 5721.371 kb on + strand, within BWI76_RS27560 at 5721.388 kb on - strand, within BWI76_RS27560 at 5721.393 kb on - strand, within BWI76_RS27560 at 5721.447 kb on + strand, within BWI76_RS27560 at 5721.457 kb on + strand, within BWI76_RS27560 at 5721.579 kb on - strand at 5721.579 kb on - strand at 5721.693 kb on - strand at 5721.708 kb on - strand at 5721.794 kb on + strand at 5721.807 kb on - strand at 5721.857 kb on + strand at 5721.857 kb on - strand, within BWI76_RS27565 at 5721.867 kb on - strand, within BWI76_RS27565 at 5721.968 kb on - strand, within BWI76_RS27565 at 5722.035 kb on + strand, within BWI76_RS27565 at 5722.056 kb on - strand, within BWI76_RS27565 at 5722.094 kb on + strand, within BWI76_RS27565 at 5722.102 kb on - strand, within BWI76_RS27565 at 5722.102 kb on - strand, within BWI76_RS27565 at 5722.102 kb on - strand, within BWI76_RS27565 at 5722.220 kb on - strand, within BWI76_RS27565 at 5722.307 kb on - strand, within BWI76_RS27565 at 5722.340 kb on + strand, within BWI76_RS27565 at 5722.350 kb on + strand, within BWI76_RS27565 at 5722.450 kb on - strand, within BWI76_RS27565
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 5,719,992 - BWI76_RS27555 0.19 -0.1 5,720,129 + BWI76_RS27555 0.33 +0.8 5,720,144 + BWI76_RS27555 0.35 -0.7 5,720,152 - BWI76_RS27555 0.36 -0.2 5,720,164 - BWI76_RS27555 0.37 -0.3 5,720,240 + BWI76_RS27555 0.45 -0.7 5,720,245 + -0.1 5,720,245 + BWI76_RS27555 0.46 +1.3 5,720,253 - BWI76_RS27555 0.47 +0.0 5,720,253 - BWI76_RS27555 0.47 -0.3 5,720,260 + BWI76_RS27555 0.47 -0.9 5,720,278 + BWI76_RS27555 0.49 +0.5 5,720,335 + BWI76_RS27555 0.55 +0.0 5,720,511 - BWI76_RS27555 0.74 -1.2 5,720,548 - BWI76_RS27555 0.78 +0.6 5,720,548 - BWI76_RS27555 0.78 +1.3 5,720,607 + BWI76_RS27555 0.85 -0.1 5,720,613 + BWI76_RS27555 0.85 +0.0 5,720,700 + +0.2 5,720,755 + -0.6 5,720,803 + -0.1 5,720,804 + -0.5 5,720,807 - +0.5 5,720,811 - +0.2 5,720,899 + BWI76_RS27560 0.11 -0.3 5,721,270 + BWI76_RS27560 0.54 -0.7 5,721,325 - BWI76_RS27560 0.61 +0.4 5,721,371 + BWI76_RS27560 0.66 +1.2 5,721,371 + BWI76_RS27560 0.66 -1.2 5,721,388 - BWI76_RS27560 0.68 +0.6 5,721,393 - BWI76_RS27560 0.69 -1.0 5,721,447 + BWI76_RS27560 0.75 +0.6 5,721,457 + BWI76_RS27560 0.76 -0.7 5,721,579 - -0.9 5,721,579 - -0.4 5,721,693 - -0.3 5,721,708 - +1.0 5,721,794 + -0.3 5,721,807 - +0.1 5,721,857 + +0.5 5,721,857 - BWI76_RS27565 0.13 +2.0 5,721,867 - BWI76_RS27565 0.15 +0.2 5,721,968 - BWI76_RS27565 0.27 +2.9 5,722,035 + BWI76_RS27565 0.34 -0.4 5,722,056 - BWI76_RS27565 0.37 -0.1 5,722,094 + BWI76_RS27565 0.41 +0.0 5,722,102 - BWI76_RS27565 0.42 -0.2 5,722,102 - BWI76_RS27565 0.42 +0.1 5,722,102 - BWI76_RS27565 0.42 -1.7 5,722,220 - BWI76_RS27565 0.56 +0.0 5,722,307 - BWI76_RS27565 0.66 -1.4 5,722,340 + BWI76_RS27565 0.70 +0.7 5,722,350 + BWI76_RS27565 0.71 +0.2 5,722,450 - BWI76_RS27565 0.83 -3.2
Or see this region's nucleotide sequence