Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27220 and BWI76_RS27225 are separated by 111 nucleotides BWI76_RS27225 and BWI76_RS27230 are separated by 245 nucleotides BWI76_RS27230 and BWI76_RS27235 are separated by 9 nucleotides
BWI76_RS27220: BWI76_RS27220 - glutaredoxin 3, at 5,649,722 to 5,649,973
_RS27220
BWI76_RS27225: BWI76_RS27225 - rhodanese-like domain-containing protein, at 5,650,085 to 5,650,516
_RS27225
BWI76_RS27230: BWI76_RS27230 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 5,650,762 to 5,652,306
_RS27230
BWI76_RS27235: BWI76_RS27235 - murein hydrolase activator EnvC, at 5,652,316 to 5,653,587
_RS27235
Position (kb) 5650
5651
5652
5653 Strain fitness (log2 ratio) -2
-1
0
1 at 5649.898 kb on - strand, within BWI76_RS27220 at 5649.958 kb on - strand at 5650.117 kb on - strand at 5650.164 kb on - strand, within BWI76_RS27225 at 5650.175 kb on - strand, within BWI76_RS27225 at 5650.472 kb on + strand, within BWI76_RS27225 at 5650.561 kb on + strand at 5650.569 kb on - strand at 5650.686 kb on - strand at 5650.861 kb on + strand at 5650.957 kb on - strand, within BWI76_RS27230 at 5650.957 kb on - strand, within BWI76_RS27230 at 5650.965 kb on + strand, within BWI76_RS27230 at 5651.007 kb on - strand, within BWI76_RS27230 at 5651.048 kb on + strand, within BWI76_RS27230 at 5651.056 kb on - strand, within BWI76_RS27230 at 5651.061 kb on - strand, within BWI76_RS27230 at 5651.118 kb on - strand, within BWI76_RS27230 at 5651.289 kb on + strand, within BWI76_RS27230 at 5651.290 kb on + strand, within BWI76_RS27230 at 5651.362 kb on + strand, within BWI76_RS27230 at 5651.400 kb on + strand, within BWI76_RS27230 at 5651.502 kb on - strand, within BWI76_RS27230 at 5651.664 kb on + strand, within BWI76_RS27230 at 5651.672 kb on - strand, within BWI76_RS27230 at 5651.966 kb on + strand, within BWI76_RS27230 at 5652.030 kb on - strand, within BWI76_RS27230 at 5652.104 kb on - strand, within BWI76_RS27230 at 5652.135 kb on + strand, within BWI76_RS27230 at 5652.135 kb on + strand, within BWI76_RS27230 at 5652.143 kb on - strand, within BWI76_RS27230 at 5652.301 kb on + strand at 5652.320 kb on + strand at 5652.395 kb on - strand at 5652.406 kb on - strand at 5652.467 kb on - strand, within BWI76_RS27235 at 5652.528 kb on + strand, within BWI76_RS27235 at 5652.528 kb on + strand, within BWI76_RS27235 at 5652.536 kb on - strand, within BWI76_RS27235 at 5652.540 kb on + strand, within BWI76_RS27235 at 5652.540 kb on + strand, within BWI76_RS27235 at 5652.546 kb on + strand, within BWI76_RS27235 at 5652.782 kb on - strand, within BWI76_RS27235 at 5652.842 kb on + strand, within BWI76_RS27235 at 5652.929 kb on - strand, within BWI76_RS27235 at 5653.100 kb on + strand, within BWI76_RS27235 at 5653.122 kb on + strand, within BWI76_RS27235 at 5653.148 kb on + strand, within BWI76_RS27235 at 5653.204 kb on - strand, within BWI76_RS27235 at 5653.266 kb on + strand, within BWI76_RS27235 at 5653.272 kb on + strand, within BWI76_RS27235
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 5,649,898 - BWI76_RS27220 0.70 -0.5 5,649,958 - -0.3 5,650,117 - +0.4 5,650,164 - BWI76_RS27225 0.18 -0.2 5,650,175 - BWI76_RS27225 0.21 +0.3 5,650,472 + BWI76_RS27225 0.90 +1.2 5,650,561 + -1.5 5,650,569 - +0.3 5,650,686 - -0.7 5,650,861 + +0.1 5,650,957 - BWI76_RS27230 0.13 -1.0 5,650,957 - BWI76_RS27230 0.13 -0.2 5,650,965 + BWI76_RS27230 0.13 +0.9 5,651,007 - BWI76_RS27230 0.16 +0.0 5,651,048 + BWI76_RS27230 0.19 +0.1 5,651,056 - BWI76_RS27230 0.19 -0.6 5,651,061 - BWI76_RS27230 0.19 -0.1 5,651,118 - BWI76_RS27230 0.23 +0.6 5,651,289 + BWI76_RS27230 0.34 +1.5 5,651,290 + BWI76_RS27230 0.34 -0.4 5,651,362 + BWI76_RS27230 0.39 -0.2 5,651,400 + BWI76_RS27230 0.41 -1.1 5,651,502 - BWI76_RS27230 0.48 +1.1 5,651,664 + BWI76_RS27230 0.58 -0.0 5,651,672 - BWI76_RS27230 0.59 +0.4 5,651,966 + BWI76_RS27230 0.78 +0.3 5,652,030 - BWI76_RS27230 0.82 +0.5 5,652,104 - BWI76_RS27230 0.87 -1.0 5,652,135 + BWI76_RS27230 0.89 +0.5 5,652,135 + BWI76_RS27230 0.89 +0.2 5,652,143 - BWI76_RS27230 0.89 -0.4 5,652,301 + -0.8 5,652,320 + -1.7 5,652,395 - -1.5 5,652,406 - -1.4 5,652,467 - BWI76_RS27235 0.12 -1.2 5,652,528 + BWI76_RS27235 0.17 -1.0 5,652,528 + BWI76_RS27235 0.17 -2.1 5,652,536 - BWI76_RS27235 0.17 -1.1 5,652,540 + BWI76_RS27235 0.18 -2.6 5,652,540 + BWI76_RS27235 0.18 -0.3 5,652,546 + BWI76_RS27235 0.18 -1.5 5,652,782 - BWI76_RS27235 0.37 +0.7 5,652,842 + BWI76_RS27235 0.41 -2.1 5,652,929 - BWI76_RS27235 0.48 -1.7 5,653,100 + BWI76_RS27235 0.62 -0.5 5,653,122 + BWI76_RS27235 0.63 -2.2 5,653,148 + BWI76_RS27235 0.65 +0.5 5,653,204 - BWI76_RS27235 0.70 -1.0 5,653,266 + BWI76_RS27235 0.75 -1.5 5,653,272 + BWI76_RS27235 0.75 -0.8
Or see this region's nucleotide sequence