Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23780 and BWI76_RS23785 are separated by 14 nucleotides BWI76_RS23785 and BWI76_RS23790 are separated by 52 nucleotides BWI76_RS23790 and BWI76_RS23795 are separated by 42 nucleotides
BWI76_RS23780: BWI76_RS23780 - aromatic acid decarboxylase, at 4,964,633 to 4,965,211
_RS23780
BWI76_RS23785: BWI76_RS23785 - hypothetical protein, at 4,965,226 to 4,965,942
_RS23785
BWI76_RS23790: BWI76_RS23790 - isopentenyl-diphosphate Delta-isomerase, at 4,965,995 to 4,966,543
_RS23790
BWI76_RS23795: BWI76_RS23795 - lysine--tRNA ligase, at 4,966,586 to 4,968,103
_RS23795
Position (kb) 4965
4966
4967 Strain fitness (log2 ratio) -1
0
1 at 4965.163 kb on + strand at 4965.178 kb on + strand at 4965.244 kb on - strand at 4965.335 kb on - strand, within BWI76_RS23785 at 4965.343 kb on - strand, within BWI76_RS23785 at 4965.358 kb on - strand, within BWI76_RS23785 at 4965.535 kb on - strand, within BWI76_RS23785 at 4965.688 kb on + strand, within BWI76_RS23785 at 4965.711 kb on - strand, within BWI76_RS23785 at 4966.034 kb on - strand at 4966.036 kb on + strand at 4966.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 4,965,163 + +0.1 4,965,178 + -0.3 4,965,244 - -1.5 4,965,335 - BWI76_RS23785 0.15 -0.2 4,965,343 - BWI76_RS23785 0.16 -0.4 4,965,358 - BWI76_RS23785 0.18 -0.4 4,965,535 - BWI76_RS23785 0.43 -0.8 4,965,688 + BWI76_RS23785 0.64 +0.4 4,965,711 - BWI76_RS23785 0.68 +0.1 4,966,034 - -0.5 4,966,036 + +0.3 4,966,491 - +0.0
Or see this region's nucleotide sequence