Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18875 and BWI76_RS18880 are separated by 23 nucleotides BWI76_RS18880 and BWI76_RS18885 are separated by 321 nucleotides BWI76_RS18885 and BWI76_RS18890 overlap by 4 nucleotides
BWI76_RS18875: BWI76_RS18875 - nitrogen fixation protein NifM, at 3,913,945 to 3,914,745
_RS18875
BWI76_RS18880: BWI76_RS18880 - flavodoxin, at 3,914,769 to 3,915,299
_RS18880
BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108
_RS18885
BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679
_RS18890
Position (kb)
3915
3916
3917
3918 Strain fitness (log2 ratio)
-1
0
1 at 3914.792 kb on + strand at 3914.813 kb on - strand at 3915.397 kb on - strand at 3915.641 kb on + strand at 3916.112 kb on + strand, within BWI76_RS18885 at 3916.779 kb on - strand, within BWI76_RS18885 at 3916.853 kb on - strand, within BWI76_RS18885 at 3917.080 kb on + strand at 3917.098 kb on - strand at 3917.189 kb on + strand at 3917.189 kb on + strand at 3917.231 kb on + strand at 3917.256 kb on + strand at 3917.486 kb on - strand, within BWI76_RS18890 at 3917.499 kb on - strand, within BWI76_RS18890 at 3917.514 kb on + strand, within BWI76_RS18890 at 3917.688 kb on - strand, within BWI76_RS18890 at 3917.688 kb on - strand, within BWI76_RS18890 at 3917.693 kb on - strand, within BWI76_RS18890 at 3917.797 kb on - strand, within BWI76_RS18890 at 3917.799 kb on + strand, within BWI76_RS18890 at 3917.810 kb on - strand, within BWI76_RS18890 at 3917.874 kb on - strand, within BWI76_RS18890 at 3918.008 kb on - strand, within BWI76_RS18890
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 3,914,792 + +0.0 3,914,813 - -0.3 3,915,397 - +0.6 3,915,641 + -0.4 3,916,112 + BWI76_RS18885 0.33 -0.8 3,916,779 - BWI76_RS18885 0.78 +0.2 3,916,853 - BWI76_RS18885 0.83 -0.9 3,917,080 + +0.0 3,917,098 - +0.8 3,917,189 + -0.4 3,917,189 + -0.5 3,917,231 + +0.1 3,917,256 + -1.3 3,917,486 - BWI76_RS18890 0.24 -0.2 3,917,499 - BWI76_RS18890 0.25 +0.4 3,917,514 + BWI76_RS18890 0.26 +0.3 3,917,688 - BWI76_RS18890 0.37 -0.8 3,917,688 - BWI76_RS18890 0.37 -0.9 3,917,693 - BWI76_RS18890 0.37 -0.4 3,917,797 - BWI76_RS18890 0.44 -0.5 3,917,799 + BWI76_RS18890 0.44 +0.3 3,917,810 - BWI76_RS18890 0.45 -0.5 3,917,874 - BWI76_RS18890 0.49 +0.4 3,918,008 - BWI76_RS18890 0.57 -0.1
Or see this region's nucleotide sequence