Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16955

Experiment: Fusidic 1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16945 and BWI76_RS16950 are separated by 126 nucleotidesBWI76_RS16950 and BWI76_RS16955 are separated by 26 nucleotidesBWI76_RS16955 and BWI76_RS16960 are separated by 509 nucleotides BWI76_RS16945: BWI76_RS16945 - 4-hydroxyphenylpyruvate dioxygenase, at 3,514,195 to 3,516,048 _RS16945 BWI76_RS16950: BWI76_RS16950 - TetR family transcriptional regulator, at 3,516,175 to 3,516,813 _RS16950 BWI76_RS16955: BWI76_RS16955 - hypothetical protein, at 3,516,840 to 3,517,052 _RS16955 BWI76_RS16960: BWI76_RS16960 - pyruvate kinase, at 3,517,562 to 3,518,974 _RS16960 Position (kb) 3516 3517 3518Strain fitness (log2 ratio) -1 0 1at 3516.302 kb on + strand, within BWI76_RS16950at 3516.427 kb on + strand, within BWI76_RS16950at 3516.469 kb on + strand, within BWI76_RS16950at 3516.965 kb on + strand, within BWI76_RS16955at 3517.113 kb on + strandat 3517.121 kb on - strandat 3517.895 kb on + strand, within BWI76_RS16960

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml
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3,516,302 + BWI76_RS16950 0.20 +1.2
3,516,427 + BWI76_RS16950 0.39 -0.4
3,516,469 + BWI76_RS16950 0.46 -0.7
3,516,965 + BWI76_RS16955 0.59 +0.0
3,517,113 + -0.1
3,517,121 - -0.3
3,517,895 + BWI76_RS16960 0.24 -1.4

Or see this region's nucleotide sequence