Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10755 and BWI76_RS10760 are separated by 9 nucleotides BWI76_RS10760 and BWI76_RS10765 overlap by 4 nucleotides BWI76_RS10765 and BWI76_RS10770 overlap by 4 nucleotides BWI76_RS10770 and BWI76_RS10775 overlap by 8 nucleotides
BWI76_RS10755: BWI76_RS10755 - pyrimidine utilization flavin reductase protein F, at 2,271,852 to 2,272,346
_RS10755
BWI76_RS10760: BWI76_RS10760 - nitroreductase family protein, at 2,272,356 to 2,272,946
_RS10760
BWI76_RS10765: BWI76_RS10765 - pyrimidine utilization protein D, at 2,272,943 to 2,273,746
_RS10765
BWI76_RS10770: BWI76_RS10770 - pyrimidine utilization protein C, at 2,273,743 to 2,274,135
_RS10770
BWI76_RS10775: BWI76_RS10775 - pyrimidine utilization protein B, at 2,274,128 to 2,274,838
_RS10775
Position (kb)
2272
2273
2274 Strain fitness (log2 ratio)
-1
0
1 at 2272.362 kb on - strand at 2272.685 kb on - strand, within BWI76_RS10760 at 2272.734 kb on + strand, within BWI76_RS10760 at 2272.741 kb on + strand, within BWI76_RS10760 at 2272.742 kb on - strand at 2273.158 kb on - strand, within BWI76_RS10765 at 2273.158 kb on - strand, within BWI76_RS10765 at 2273.403 kb on - strand, within BWI76_RS10765 at 2273.463 kb on - strand, within BWI76_RS10765 at 2273.851 kb on - strand, within BWI76_RS10770 at 2273.922 kb on + strand, within BWI76_RS10770 at 2274.148 kb on + strand at 2274.172 kb on + strand at 2274.274 kb on - strand, within BWI76_RS10775 at 2274.279 kb on + strand, within BWI76_RS10775 at 2274.392 kb on + strand, within BWI76_RS10775 at 2274.414 kb on + strand, within BWI76_RS10775 at 2274.529 kb on + strand, within BWI76_RS10775 at 2274.537 kb on - strand, within BWI76_RS10775 at 2274.735 kb on - strand, within BWI76_RS10775
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 2,272,362 - -0.9 2,272,685 - BWI76_RS10760 0.56 -0.5 2,272,734 + BWI76_RS10760 0.64 +0.8 2,272,741 + BWI76_RS10760 0.65 +0.1 2,272,742 - -0.4 2,273,158 - BWI76_RS10765 0.27 -0.6 2,273,158 - BWI76_RS10765 0.27 +0.5 2,273,403 - BWI76_RS10765 0.57 +0.0 2,273,463 - BWI76_RS10765 0.65 +0.0 2,273,851 - BWI76_RS10770 0.27 -0.4 2,273,922 + BWI76_RS10770 0.46 +0.6 2,274,148 + -0.0 2,274,172 + +0.5 2,274,274 - BWI76_RS10775 0.21 +0.1 2,274,279 + BWI76_RS10775 0.21 -0.2 2,274,392 + BWI76_RS10775 0.37 +0.1 2,274,414 + BWI76_RS10775 0.40 +0.4 2,274,529 + BWI76_RS10775 0.56 -0.4 2,274,537 - BWI76_RS10775 0.58 -0.8 2,274,735 - BWI76_RS10775 0.85 +0.1
Or see this region's nucleotide sequence