Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09760 and BWI76_RS09765 are separated by 147 nucleotides BWI76_RS09765 and BWI76_RS09770 overlap by 54 nucleotides BWI76_RS09770 and BWI76_RS09775 overlap by 68 nucleotides BWI76_RS09775 and BWI76_RS09780 are separated by 137 nucleotides
BWI76_RS09760: BWI76_RS09760 - 3-phosphoshikimate 1-carboxyvinyltransferase, at 2,047,316 to 2,048,599
_RS09760
BWI76_RS09765: BWI76_RS09765 - metalloprotease, at 2,048,747 to 2,049,514
_RS09765
BWI76_RS09770: BWI76_RS09770 - hypothetical protein, at 2,049,461 to 2,049,753
_RS09770
BWI76_RS09775: BWI76_RS09775 - cytidylate kinase, at 2,049,686 to 2,050,369
_RS09775
BWI76_RS09780: BWI76_RS09780 - 30S ribosomal protein S1, at 2,050,507 to 2,052,180
_RS09780
Position (kb)
2048
2049
2050 Strain fitness (log2 ratio)
-1
0
1 at 2048.002 kb on + strand, within BWI76_RS09760 at 2048.002 kb on + strand, within BWI76_RS09760 at 2048.002 kb on + strand, within BWI76_RS09760 at 2048.119 kb on - strand, within BWI76_RS09760 at 2048.366 kb on + strand, within BWI76_RS09760 at 2048.434 kb on + strand, within BWI76_RS09760 at 2048.593 kb on + strand at 2048.593 kb on + strand at 2048.837 kb on - strand, within BWI76_RS09765 at 2049.152 kb on - strand, within BWI76_RS09765 at 2049.624 kb on - strand, within BWI76_RS09770
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 2,048,002 + BWI76_RS09760 0.53 -0.9 2,048,002 + BWI76_RS09760 0.53 +0.3 2,048,002 + BWI76_RS09760 0.53 -0.2 2,048,119 - BWI76_RS09760 0.63 +1.7 2,048,366 + BWI76_RS09760 0.82 +0.2 2,048,434 + BWI76_RS09760 0.87 +0.0 2,048,593 + -0.1 2,048,593 + -1.0 2,048,837 - BWI76_RS09765 0.12 -0.6 2,049,152 - BWI76_RS09765 0.53 -0.8 2,049,624 - BWI76_RS09770 0.56 -0.1
Or see this region's nucleotide sequence