Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04080 and BWI76_RS04085 are separated by 158 nucleotides BWI76_RS04085 and BWI76_RS04090 overlap by 32 nucleotides BWI76_RS04090 and BWI76_RS04095 are separated by 14 nucleotides BWI76_RS04095 and BWI76_RS04100 are separated by 91 nucleotides
BWI76_RS04080: BWI76_RS04080 - 16S rRNA (guanine(1207)-N(2))-methyltransferase, at 840,258 to 841,286
_RS04080
BWI76_RS04085: BWI76_RS04085 - DNA polymerase III subunit psi, at 841,445 to 841,858
_RS04085
BWI76_RS04090: BWI76_RS04090 - ribosomal-protein-alanine N-acetyltransferase RimI, at 841,827 to 842,273
_RS04090
BWI76_RS04095: BWI76_RS04095 - dUMP phosphatase, at 842,288 to 842,965
_RS04095
BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646
_RS04100
Position (kb) 841
842
843 Strain fitness (log2 ratio) -1
0
1 at 840.863 kb on + strand, within BWI76_RS04080 at 840.930 kb on + strand, within BWI76_RS04080 at 840.933 kb on - strand, within BWI76_RS04080 at 840.938 kb on - strand, within BWI76_RS04080 at 840.966 kb on + strand, within BWI76_RS04080 at 841.008 kb on + strand, within BWI76_RS04080 at 841.021 kb on + strand, within BWI76_RS04080 at 841.124 kb on + strand, within BWI76_RS04080 at 841.173 kb on + strand, within BWI76_RS04080 at 841.271 kb on + strand at 841.857 kb on + strand at 841.983 kb on + strand, within BWI76_RS04090 at 842.066 kb on + strand, within BWI76_RS04090 at 842.076 kb on - strand, within BWI76_RS04090 at 842.128 kb on + strand, within BWI76_RS04090 at 842.143 kb on + strand, within BWI76_RS04090 at 842.730 kb on - strand, within BWI76_RS04095 at 842.876 kb on - strand, within BWI76_RS04095
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 840,863 + BWI76_RS04080 0.59 +1.0 840,930 + BWI76_RS04080 0.65 +1.2 840,933 - BWI76_RS04080 0.66 +0.3 840,938 - BWI76_RS04080 0.66 -0.9 840,966 + BWI76_RS04080 0.69 +1.7 841,008 + BWI76_RS04080 0.73 +0.5 841,021 + BWI76_RS04080 0.74 -1.1 841,124 + BWI76_RS04080 0.84 +0.4 841,173 + BWI76_RS04080 0.89 -0.5 841,271 + +0.4 841,857 + -1.1 841,983 + BWI76_RS04090 0.35 -0.4 842,066 + BWI76_RS04090 0.53 -1.2 842,076 - BWI76_RS04090 0.56 -1.3 842,128 + BWI76_RS04090 0.67 +0.4 842,143 + BWI76_RS04090 0.71 -1.7 842,730 - BWI76_RS04095 0.65 -0.0 842,876 - BWI76_RS04095 0.87 +0.2
Or see this region's nucleotide sequence