Experiment: Fusidic 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02430 and BWI76_RS02435 overlap by 4 nucleotides BWI76_RS02435 and BWI76_RS02440 are separated by 140 nucleotides BWI76_RS02440 and BWI76_RS02445 are separated by 10 nucleotides BWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotides
BWI76_RS02430: BWI76_RS02430 - multidrug transporter, at 499,179 to 499,496
_RS02430
BWI76_RS02435: BWI76_RS02435 - outer membrane lipoprotein Blc, at 499,493 to 500,023
_RS02435
BWI76_RS02440: BWI76_RS02440 - fumarate reductase subunit D, at 500,164 to 500,523
_RS02440
BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929
_RS02445
BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674
_RS02450
Position (kb) 500
501 Strain fitness (log2 ratio) -2
-1
0
1
2 at 499.196 kb on + strand at 499.202 kb on - strand at 499.211 kb on + strand, within BWI76_RS02430 at 499.211 kb on + strand, within BWI76_RS02430 at 499.219 kb on - strand, within BWI76_RS02430 at 499.220 kb on + strand, within BWI76_RS02430 at 499.220 kb on + strand, within BWI76_RS02430 at 499.308 kb on + strand, within BWI76_RS02430 at 499.324 kb on - strand, within BWI76_RS02430 at 499.359 kb on + strand, within BWI76_RS02430 at 499.392 kb on - strand, within BWI76_RS02430 at 499.397 kb on - strand, within BWI76_RS02430 at 499.468 kb on + strand at 499.491 kb on + strand at 499.499 kb on - strand at 499.510 kb on + strand at 499.606 kb on + strand, within BWI76_RS02435 at 499.614 kb on - strand, within BWI76_RS02435 at 499.828 kb on + strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.836 kb on - strand, within BWI76_RS02435 at 499.934 kb on - strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 499.960 kb on + strand, within BWI76_RS02435 at 500.052 kb on + strand at 500.299 kb on - strand, within BWI76_RS02440 at 500.304 kb on + strand, within BWI76_RS02440 at 500.463 kb on - strand, within BWI76_RS02440 at 500.543 kb on - strand at 500.543 kb on - strand at 500.586 kb on + strand, within BWI76_RS02445 at 500.762 kb on + strand, within BWI76_RS02445 at 500.848 kb on + strand, within BWI76_RS02445 at 500.850 kb on + strand, within BWI76_RS02445 at 500.911 kb on - strand at 500.948 kb on + strand at 500.956 kb on - strand at 500.994 kb on - strand at 501.287 kb on + strand, within BWI76_RS02450 at 501.420 kb on - strand, within BWI76_RS02450 at 501.475 kb on - strand, within BWI76_RS02450 at 501.505 kb on + strand, within BWI76_RS02450
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 1 mg/ml remove 499,196 + +0.0 499,202 - +0.5 499,211 + BWI76_RS02430 0.10 -0.2 499,211 + BWI76_RS02430 0.10 -0.0 499,219 - BWI76_RS02430 0.13 -0.2 499,220 + BWI76_RS02430 0.13 +0.2 499,220 + BWI76_RS02430 0.13 +0.5 499,308 + BWI76_RS02430 0.41 +0.6 499,324 - BWI76_RS02430 0.46 +0.1 499,359 + BWI76_RS02430 0.57 -1.3 499,392 - BWI76_RS02430 0.67 +1.0 499,397 - BWI76_RS02430 0.69 -0.1 499,468 + -2.7 499,491 + +0.3 499,499 - -0.1 499,510 + -0.9 499,606 + BWI76_RS02435 0.21 -1.5 499,614 - BWI76_RS02435 0.23 +0.3 499,828 + BWI76_RS02435 0.63 -0.3 499,836 - BWI76_RS02435 0.65 +1.2 499,836 - BWI76_RS02435 0.65 -0.3 499,836 - BWI76_RS02435 0.65 -0.2 499,934 - BWI76_RS02435 0.83 -0.9 499,960 + BWI76_RS02435 0.88 -1.0 499,960 + BWI76_RS02435 0.88 -0.1 499,960 + BWI76_RS02435 0.88 -0.1 500,052 + -0.9 500,299 - BWI76_RS02440 0.38 +0.2 500,304 + BWI76_RS02440 0.39 +0.7 500,463 - BWI76_RS02440 0.83 -0.2 500,543 - +0.2 500,543 - -0.1 500,586 + BWI76_RS02445 0.13 +2.0 500,762 + BWI76_RS02445 0.58 -0.2 500,848 + BWI76_RS02445 0.79 +0.1 500,850 + BWI76_RS02445 0.80 -0.3 500,911 - +0.6 500,948 + +0.5 500,956 - +0.1 500,994 - -0.5 501,287 + BWI76_RS02450 0.47 -0.2 501,420 - BWI76_RS02450 0.65 +0.3 501,475 - BWI76_RS02450 0.73 -0.1 501,505 + BWI76_RS02450 0.77 -1.2
Or see this region's nucleotide sequence