Experiment: Citric Acid (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0165 and Psyr_0166 are separated by 563 nucleotides Psyr_0166 and Psyr_0167 are separated by 5 nucleotides Psyr_0167 and Psyr_0168 are separated by 370 nucleotides
Psyr_0165: Psyr_0165 - GCN5-related N-acetyltransferase, at 179,100 to 179,543
_0165
Psyr_0166: Psyr_0166 - hypothetical protein, at 180,107 to 181,417
_0166
Psyr_0167: Psyr_0167 - hypothetical protein, at 181,423 to 181,845
_0167
Psyr_0168: Psyr_0168 - Phosphoenolpyruvate carboxykinase (ATP), at 182,216 to 183,760
_0168
Position (kb) 180
181
182 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.128 kb on + strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.129 kb on - strand at 179.209 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.253 kb on + strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.254 kb on - strand, within Psyr_0165 at 179.399 kb on + strand, within Psyr_0165 at 179.424 kb on + strand, within Psyr_0165 at 179.425 kb on - strand, within Psyr_0165 at 179.458 kb on + strand, within Psyr_0165 at 179.733 kb on - strand at 179.769 kb on + strand at 179.769 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.862 kb on + strand at 179.863 kb on - strand at 179.863 kb on - strand at 179.866 kb on + strand at 179.866 kb on + strand at 179.866 kb on + strand at 179.867 kb on - strand at 179.867 kb on - strand at 179.877 kb on + strand at 179.927 kb on - strand at 180.013 kb on + strand at 180.014 kb on - strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.065 kb on + strand at 180.066 kb on - strand at 180.066 kb on - strand at 180.066 kb on - strand at 180.076 kb on + strand at 180.077 kb on - strand at 180.077 kb on - strand at 180.140 kb on + strand at 180.140 kb on + strand at 180.140 kb on + strand at 180.141 kb on - strand at 180.141 kb on - strand at 180.141 kb on - strand at 180.175 kb on - strand at 180.195 kb on + strand at 180.237 kb on - strand at 180.268 kb on - strand, within Psyr_0166 at 180.399 kb on + strand, within Psyr_0166 at 180.418 kb on + strand, within Psyr_0166 at 180.473 kb on - strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.529 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.533 kb on + strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.534 kb on - strand, within Psyr_0166 at 180.722 kb on - strand, within Psyr_0166 at 180.748 kb on + strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.847 kb on - strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.995 kb on + strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.996 kb on - strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 180.997 kb on + strand, within Psyr_0166 at 181.012 kb on + strand, within Psyr_0166 at 181.016 kb on + strand, within Psyr_0166 at 181.017 kb on - strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.042 kb on + strand, within Psyr_0166 at 181.092 kb on - strand, within Psyr_0166 at 181.151 kb on - strand, within Psyr_0166 at 181.184 kb on + strand, within Psyr_0166 at 181.274 kb on + strand, within Psyr_0166 at 181.286 kb on + strand, within Psyr_0166 at 181.287 kb on - strand at 181.288 kb on + strand at 181.289 kb on - strand at 181.328 kb on - strand at 181.387 kb on + strand at 181.611 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.693 kb on + strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.694 kb on - strand, within Psyr_0167 at 181.696 kb on - strand, within Psyr_0167 at 181.798 kb on - strand, within Psyr_0167 at 181.843 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.847 kb on + strand at 181.848 kb on - strand at 181.848 kb on - strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.904 kb on + strand at 181.905 kb on - strand at 182.034 kb on - strand at 182.054 kb on - strand at 182.084 kb on + strand at 182.086 kb on + strand at 182.087 kb on - strand at 182.087 kb on - strand at 182.104 kb on - strand at 182.128 kb on - strand at 182.207 kb on + strand at 182.207 kb on + strand at 182.208 kb on - strand at 182.217 kb on + strand at 182.218 kb on - strand at 182.218 kb on - strand at 182.313 kb on + strand at 182.314 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Citric Acid (C) remove 179,128 + -0.1 179,128 + +0.6 179,128 + +0.2 179,128 + -0.3 179,128 + -0.6 179,128 + -2.2 179,128 + -0.1 179,128 + +0.6 179,128 + -1.4 179,128 + +0.4 179,128 + -0.5 179,129 - -0.2 179,129 - +1.1 179,129 - +0.1 179,129 - +1.5 179,129 - +0.6 179,129 - -1.6 179,129 - +0.4 179,129 - -0.2 179,209 + Psyr_0165 0.25 +0.2 179,253 + Psyr_0165 0.34 -1.0 179,253 + Psyr_0165 0.34 +0.7 179,253 + Psyr_0165 0.34 +0.0 179,253 + Psyr_0165 0.34 +0.4 179,253 + Psyr_0165 0.34 -0.4 179,254 - Psyr_0165 0.35 -0.3 179,254 - Psyr_0165 0.35 +0.3 179,399 + Psyr_0165 0.67 +1.4 179,424 + Psyr_0165 0.73 +0.1 179,425 - Psyr_0165 0.73 +0.5 179,458 + Psyr_0165 0.81 -0.3 179,733 - +0.4 179,769 + -0.2 179,769 + +0.3 179,862 + +0.8 179,862 + +0.7 179,862 + +0.4 179,862 + +0.2 179,863 - -0.2 179,863 - +0.1 179,866 + +1.5 179,866 + -0.3 179,866 + -0.6 179,867 - +0.6 179,867 - +0.6 179,877 + +0.4 179,927 - -1.1 180,013 + +1.3 180,014 - +0.4 180,065 + -1.1 180,065 + +0.2 180,065 + -0.9 180,065 + -0.8 180,066 - -0.6 180,066 - -0.2 180,066 - -0.4 180,076 + -1.6 180,077 - +0.0 180,077 - -0.6 180,140 + -0.8 180,140 + -0.1 180,140 + +0.4 180,141 - +0.6 180,141 - -0.9 180,141 - +0.1 180,175 - -0.5 180,195 + -0.3 180,237 - -0.6 180,268 - Psyr_0166 0.12 +0.5 180,399 + Psyr_0166 0.22 -0.4 180,418 + Psyr_0166 0.24 -0.3 180,473 - Psyr_0166 0.28 -0.2 180,529 + Psyr_0166 0.32 +1.1 180,529 + Psyr_0166 0.32 -0.3 180,533 + Psyr_0166 0.32 +1.3 180,533 + Psyr_0166 0.32 -1.6 180,534 - Psyr_0166 0.33 -0.4 180,534 - Psyr_0166 0.33 -0.5 180,534 - Psyr_0166 0.33 -0.0 180,534 - Psyr_0166 0.33 -0.6 180,534 - Psyr_0166 0.33 -1.8 180,534 - Psyr_0166 0.33 +0.4 180,534 - Psyr_0166 0.33 -0.3 180,722 - Psyr_0166 0.47 -1.9 180,748 + Psyr_0166 0.49 +1.3 180,847 - Psyr_0166 0.56 +0.1 180,847 - Psyr_0166 0.56 -1.2 180,995 + Psyr_0166 0.68 +3.2 180,995 + Psyr_0166 0.68 -0.1 180,995 + Psyr_0166 0.68 +2.3 180,995 + Psyr_0166 0.68 +0.1 180,996 - Psyr_0166 0.68 +0.4 180,996 - Psyr_0166 0.68 -1.3 180,997 + Psyr_0166 0.68 -0.5 180,997 + Psyr_0166 0.68 +0.4 180,997 + Psyr_0166 0.68 +0.1 180,997 + Psyr_0166 0.68 -0.1 181,012 + Psyr_0166 0.69 -0.7 181,016 + Psyr_0166 0.69 +0.1 181,017 - Psyr_0166 0.69 -2.1 181,042 + Psyr_0166 0.71 -0.8 181,042 + Psyr_0166 0.71 +0.7 181,092 - Psyr_0166 0.75 -1.6 181,151 - Psyr_0166 0.80 -2.8 181,184 + Psyr_0166 0.82 -0.5 181,274 + Psyr_0166 0.89 -0.2 181,286 + Psyr_0166 0.90 +0.9 181,287 - +0.4 181,288 + +0.1 181,289 - -0.8 181,328 - -0.9 181,387 + -0.5 181,611 + Psyr_0167 0.44 -0.8 181,693 + Psyr_0167 0.64 +0.1 181,693 + Psyr_0167 0.64 +1.4 181,693 + Psyr_0167 0.64 +1.1 181,693 + Psyr_0167 0.64 +1.4 181,694 - Psyr_0167 0.64 -0.3 181,694 - Psyr_0167 0.64 +0.7 181,696 - Psyr_0167 0.65 +1.0 181,798 - Psyr_0167 0.89 -1.8 181,843 + +1.4 181,847 + -0.2 181,847 + -1.8 181,847 + +1.4 181,848 - +0.8 181,848 - +1.7 181,904 + +0.4 181,904 + -1.2 181,904 + +0.6 181,905 - -0.4 182,034 - -2.2 182,054 - +0.4 182,084 + +0.5 182,086 + +1.4 182,087 - +0.6 182,087 - +0.7 182,104 - -1.6 182,128 - +0.4 182,207 + -1.1 182,207 + -3.4 182,208 - -0.0 182,217 + +0.9 182,218 - +0.6 182,218 - -1.2 182,313 + -2.4 182,314 - -2.6
Or see this region's nucleotide sequence