Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4587 and Pf6N2E2_4588 are separated by 73 nucleotides Pf6N2E2_4588 and Pf6N2E2_4589 are separated by 79 nucleotides Pf6N2E2_4589 and Pf6N2E2_4590 are separated by 54 nucleotides Pf6N2E2_4590 and Pf6N2E2_4591 are separated by 9 nucleotides Pf6N2E2_4591 and Pf6N2E2_4592 are separated by 144 nucleotides
Pf6N2E2_4587 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 5,317,569 to 5,318,291
_4587
Pf6N2E2_4588 - COG2363, at 5,318,365 to 5,318,745
_4588
Pf6N2E2_4589 - Sulfur carrier protein ThiS, at 5,318,825 to 5,319,043
_4589
Pf6N2E2_4590 - Thiazole biosynthesis protein ThiG, at 5,319,098 to 5,319,892
_4590
Pf6N2E2_4591 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 5,319,902 to 5,320,627
_4591
Pf6N2E2_4592 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 5,320,772 to 5,321,095
_4592
Position (kb)
5319
5320 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 5318.280 kb on + strand at 5318.281 kb on - strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.292 kb on - strand at 5318.327 kb on + strand at 5318.328 kb on - strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.373 kb on + strand at 5318.373 kb on + strand at 5318.374 kb on - strand at 5318.374 kb on - strand at 5318.467 kb on + strand, within Pf6N2E2_4588 at 5318.467 kb on + strand, within Pf6N2E2_4588 at 5318.468 kb on - strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.512 kb on - strand, within Pf6N2E2_4588 at 5318.512 kb on - strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.759 kb on + strand at 5318.759 kb on + strand at 5318.760 kb on - strand at 5318.760 kb on - strand at 5318.760 kb on - strand at 5318.805 kb on + strand at 5318.805 kb on + strand at 5319.142 kb on + strand at 5319.142 kb on + strand at 5319.143 kb on - strand at 5319.180 kb on - strand, within Pf6N2E2_4590 at 5319.314 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.515 kb on + strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.516 kb on - strand, within Pf6N2E2_4590 at 5319.624 kb on - strand, within Pf6N2E2_4590 at 5319.624 kb on - strand, within Pf6N2E2_4590 at 5319.821 kb on + strand at 5319.852 kb on - strand at 5319.890 kb on + strand at 5319.890 kb on + strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5319.891 kb on - strand at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.457 kb on + strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.458 kb on - strand, within Pf6N2E2_4591 at 5320.466 kb on + strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.467 kb on - strand, within Pf6N2E2_4591 at 5320.626 kb on - strand at 5320.690 kb on + strand at 5320.690 kb on + strand at 5320.691 kb on - strand at 5320.691 kb on - strand at 5320.716 kb on + strand at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.836 kb on + strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.837 kb on - strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.881 kb on + strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592 at 5320.882 kb on - strand, within Pf6N2E2_4592
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 5,318,280 + -0.1 5,318,281 - -0.2 5,318,291 + -0.1 5,318,291 + +0.4 5,318,291 + +0.9 5,318,291 + -1.1 5,318,291 + +0.2 5,318,292 - +0.3 5,318,327 + -0.6 5,318,328 - +0.7 5,318,329 + -0.5 5,318,329 + +0.5 5,318,329 + -3.0 5,318,329 + -0.6 5,318,329 + -0.0 5,318,329 + +0.1 5,318,329 + -0.6 5,318,329 + +1.1 5,318,329 + -1.0 5,318,329 + -0.6 5,318,329 + -1.3 5,318,329 + -1.7 5,318,329 + -1.2 5,318,330 - +0.8 5,318,330 - -0.7 5,318,330 - +0.5 5,318,330 - +0.8 5,318,330 - +1.7 5,318,330 - +0.9 5,318,330 - +0.4 5,318,330 - +0.6 5,318,330 - +2.4 5,318,330 - +0.9 5,318,373 + -4.5 5,318,373 + -2.0 5,318,374 - -1.0 5,318,374 - -1.3 5,318,467 + Pf6N2E2_4588 0.27 +0.3 5,318,467 + Pf6N2E2_4588 0.27 -1.1 5,318,468 - Pf6N2E2_4588 0.27 -0.5 5,318,497 + Pf6N2E2_4588 0.35 -0.8 5,318,497 + Pf6N2E2_4588 0.35 +0.9 5,318,497 + Pf6N2E2_4588 0.35 +0.6 5,318,497 + Pf6N2E2_4588 0.35 -0.0 5,318,497 + Pf6N2E2_4588 0.35 +1.4 5,318,498 - Pf6N2E2_4588 0.35 +0.0 5,318,498 - Pf6N2E2_4588 0.35 -0.2 5,318,498 - Pf6N2E2_4588 0.35 +0.7 5,318,498 - Pf6N2E2_4588 0.35 -1.9 5,318,498 - Pf6N2E2_4588 0.35 -0.0 5,318,512 - Pf6N2E2_4588 0.39 +0.6 5,318,512 - Pf6N2E2_4588 0.39 +3.2 5,318,603 + Pf6N2E2_4588 0.62 +0.9 5,318,603 + Pf6N2E2_4588 0.62 +1.8 5,318,603 + Pf6N2E2_4588 0.62 -1.1 5,318,603 + Pf6N2E2_4588 0.62 +1.0 5,318,603 + Pf6N2E2_4588 0.62 -1.6 5,318,603 + Pf6N2E2_4588 0.62 -0.2 5,318,603 + Pf6N2E2_4588 0.62 +1.2 5,318,603 + Pf6N2E2_4588 0.62 -0.9 5,318,603 + Pf6N2E2_4588 0.62 +0.2 5,318,603 + Pf6N2E2_4588 0.62 -0.5 5,318,603 + Pf6N2E2_4588 0.62 -0.6 5,318,604 - Pf6N2E2_4588 0.63 +0.2 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 -0.5 5,318,604 - Pf6N2E2_4588 0.63 +1.1 5,318,604 - Pf6N2E2_4588 0.63 -1.6 5,318,604 - Pf6N2E2_4588 0.63 -0.8 5,318,604 - Pf6N2E2_4588 0.63 -0.4 5,318,604 - Pf6N2E2_4588 0.63 -0.2 5,318,604 - Pf6N2E2_4588 0.63 +2.0 5,318,604 - Pf6N2E2_4588 0.63 -1.3 5,318,604 - Pf6N2E2_4588 0.63 +1.3 5,318,604 - Pf6N2E2_4588 0.63 -1.3 5,318,604 - Pf6N2E2_4588 0.63 +0.9 5,318,623 + Pf6N2E2_4588 0.68 +1.0 5,318,623 + Pf6N2E2_4588 0.68 +0.3 5,318,623 + Pf6N2E2_4588 0.68 +0.6 5,318,624 - Pf6N2E2_4588 0.68 -0.6 5,318,624 - Pf6N2E2_4588 0.68 -0.1 5,318,624 - Pf6N2E2_4588 0.68 -0.1 5,318,759 + +0.6 5,318,759 + -0.1 5,318,760 - -0.9 5,318,760 - +1.2 5,318,760 - +0.9 5,318,805 + -0.0 5,318,805 + +0.9 5,319,142 + -0.3 5,319,142 + +0.1 5,319,143 - -2.0 5,319,180 - Pf6N2E2_4590 0.10 -0.6 5,319,314 + Pf6N2E2_4590 0.27 -1.3 5,319,515 + Pf6N2E2_4590 0.52 -1.2 5,319,515 + Pf6N2E2_4590 0.52 -2.6 5,319,515 + Pf6N2E2_4590 0.52 -0.1 5,319,516 - Pf6N2E2_4590 0.53 -1.0 5,319,516 - Pf6N2E2_4590 0.53 -3.4 5,319,516 - Pf6N2E2_4590 0.53 -1.3 5,319,624 - Pf6N2E2_4590 0.66 -2.5 5,319,624 - Pf6N2E2_4590 0.66 -0.4 5,319,821 + -1.0 5,319,852 - +0.2 5,319,890 + +0.9 5,319,890 + +0.5 5,319,891 - -0.7 5,319,891 - -0.8 5,319,891 - +1.7 5,319,891 - -2.0 5,319,891 - -0.7 5,319,891 - +0.1 5,320,457 + Pf6N2E2_4591 0.76 -2.8 5,320,457 + Pf6N2E2_4591 0.76 +1.8 5,320,457 + Pf6N2E2_4591 0.76 -1.0 5,320,457 + Pf6N2E2_4591 0.76 +0.2 5,320,458 - Pf6N2E2_4591 0.77 -4.3 5,320,458 - Pf6N2E2_4591 0.77 +0.9 5,320,466 + Pf6N2E2_4591 0.78 -0.3 5,320,467 - Pf6N2E2_4591 0.78 -0.6 5,320,467 - Pf6N2E2_4591 0.78 -0.8 5,320,467 - Pf6N2E2_4591 0.78 +1.2 5,320,467 - Pf6N2E2_4591 0.78 +0.5 5,320,467 - Pf6N2E2_4591 0.78 +2.3 5,320,467 - Pf6N2E2_4591 0.78 -0.1 5,320,467 - Pf6N2E2_4591 0.78 -1.2 5,320,626 - +0.4 5,320,690 + +0.9 5,320,690 + -0.6 5,320,691 - -2.0 5,320,691 - -1.5 5,320,716 + +0.6 5,320,836 + Pf6N2E2_4592 0.20 -1.5 5,320,836 + Pf6N2E2_4592 0.20 +1.5 5,320,836 + Pf6N2E2_4592 0.20 +0.1 5,320,836 + Pf6N2E2_4592 0.20 -1.1 5,320,836 + Pf6N2E2_4592 0.20 +0.3 5,320,836 + Pf6N2E2_4592 0.20 +0.2 5,320,837 - Pf6N2E2_4592 0.20 -0.4 5,320,837 - Pf6N2E2_4592 0.20 -1.0 5,320,837 - Pf6N2E2_4592 0.20 +1.6 5,320,837 - Pf6N2E2_4592 0.20 +0.3 5,320,837 - Pf6N2E2_4592 0.20 -2.0 5,320,837 - Pf6N2E2_4592 0.20 -0.1 5,320,837 - Pf6N2E2_4592 0.20 -2.3 5,320,837 - Pf6N2E2_4592 0.20 +1.0 5,320,881 + Pf6N2E2_4592 0.34 +0.6 5,320,881 + Pf6N2E2_4592 0.34 +0.6 5,320,881 + Pf6N2E2_4592 0.34 -2.9 5,320,882 - Pf6N2E2_4592 0.34 +0.6 5,320,882 - Pf6N2E2_4592 0.34 -0.2 5,320,882 - Pf6N2E2_4592 0.34 +0.9 5,320,882 - Pf6N2E2_4592 0.34 +0.8
Or see this region's nucleotide sequence