Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4580 and Pf6N2E2_4581 overlap by 8 nucleotides Pf6N2E2_4581 and Pf6N2E2_4582 are separated by 155 nucleotides Pf6N2E2_4582 and Pf6N2E2_4583 are separated by 57 nucleotides
Pf6N2E2_4580 - FIG015287: Zinc protease, at 5,310,113 to 5,311,603
_4580
Pf6N2E2_4581 - FIG015547: peptidase, M16 family, at 5,311,596 to 5,312,951
_4581
Pf6N2E2_4582 - hypothetical protein, at 5,313,107 to 5,313,238
_4582
Pf6N2E2_4583 - Signal recognition particle receptor protein FtsY (=alpha subunit) (TC 3.A.5.1.1), at 5,313,296 to 5,314,729
_4583
Position (kb)
5311
5312
5313 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5310.651 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.954 kb on + strand, within Pf6N2E2_4580 at 5310.955 kb on - strand, within Pf6N2E2_4580 at 5310.970 kb on - strand, within Pf6N2E2_4580 at 5311.373 kb on + strand, within Pf6N2E2_4580 at 5311.373 kb on + strand, within Pf6N2E2_4580 at 5311.373 kb on - strand, within Pf6N2E2_4580 at 5311.374 kb on - strand, within Pf6N2E2_4580 at 5311.527 kb on + strand at 5311.527 kb on + strand at 5311.528 kb on - strand at 5311.528 kb on - strand at 5311.657 kb on + strand at 5311.657 kb on + strand at 5311.658 kb on - strand at 5311.658 kb on - strand at 5311.658 kb on - strand at 5311.679 kb on + strand at 5311.680 kb on - strand at 5311.798 kb on + strand, within Pf6N2E2_4581 at 5311.798 kb on + strand, within Pf6N2E2_4581 at 5311.798 kb on + strand, within Pf6N2E2_4581 at 5311.798 kb on + strand, within Pf6N2E2_4581 at 5311.798 kb on + strand, within Pf6N2E2_4581 at 5311.799 kb on - strand, within Pf6N2E2_4581 at 5311.799 kb on - strand, within Pf6N2E2_4581 at 5311.799 kb on - strand, within Pf6N2E2_4581 at 5311.972 kb on + strand, within Pf6N2E2_4581 at 5311.973 kb on - strand, within Pf6N2E2_4581 at 5311.973 kb on - strand, within Pf6N2E2_4581 at 5311.981 kb on + strand, within Pf6N2E2_4581 at 5311.981 kb on + strand, within Pf6N2E2_4581 at 5311.982 kb on - strand, within Pf6N2E2_4581 at 5311.982 kb on - strand, within Pf6N2E2_4581 at 5312.255 kb on - strand, within Pf6N2E2_4581 at 5312.330 kb on - strand, within Pf6N2E2_4581 at 5312.450 kb on - strand, within Pf6N2E2_4581 at 5312.608 kb on + strand, within Pf6N2E2_4581 at 5312.608 kb on + strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.609 kb on - strand, within Pf6N2E2_4581 at 5312.611 kb on + strand, within Pf6N2E2_4581 at 5312.611 kb on + strand, within Pf6N2E2_4581 at 5312.612 kb on - strand, within Pf6N2E2_4581 at 5312.612 kb on - strand, within Pf6N2E2_4581 at 5312.761 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5312.779 kb on + strand, within Pf6N2E2_4581 at 5313.011 kb on + strand at 5313.057 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.240 kb on + strand at 5313.244 kb on + strand at 5313.244 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 5,310,651 + Pf6N2E2_4580 0.36 -0.8 5,310,954 + Pf6N2E2_4580 0.56 -2.7 5,310,954 + Pf6N2E2_4580 0.56 +0.8 5,310,954 + Pf6N2E2_4580 0.56 -2.6 5,310,954 + Pf6N2E2_4580 0.56 +0.1 5,310,954 + Pf6N2E2_4580 0.56 -0.5 5,310,955 - Pf6N2E2_4580 0.56 -0.5 5,310,970 - Pf6N2E2_4580 0.57 +1.7 5,311,373 + Pf6N2E2_4580 0.85 +0.3 5,311,373 + Pf6N2E2_4580 0.85 -0.9 5,311,373 - Pf6N2E2_4580 0.85 +0.1 5,311,374 - Pf6N2E2_4580 0.85 +0.9 5,311,527 + -0.1 5,311,527 + -2.2 5,311,528 - -2.9 5,311,528 - +0.7 5,311,657 + -0.3 5,311,657 + +0.3 5,311,658 - -1.5 5,311,658 - -0.4 5,311,658 - +0.7 5,311,679 + -1.9 5,311,680 - -0.6 5,311,798 + Pf6N2E2_4581 0.15 +0.5 5,311,798 + Pf6N2E2_4581 0.15 +0.7 5,311,798 + Pf6N2E2_4581 0.15 -1.9 5,311,798 + Pf6N2E2_4581 0.15 -1.3 5,311,798 + Pf6N2E2_4581 0.15 +0.3 5,311,799 - Pf6N2E2_4581 0.15 +0.0 5,311,799 - Pf6N2E2_4581 0.15 +0.0 5,311,799 - Pf6N2E2_4581 0.15 -0.0 5,311,972 + Pf6N2E2_4581 0.28 -1.3 5,311,973 - Pf6N2E2_4581 0.28 +0.4 5,311,973 - Pf6N2E2_4581 0.28 +0.1 5,311,981 + Pf6N2E2_4581 0.28 -0.6 5,311,981 + Pf6N2E2_4581 0.28 -2.4 5,311,982 - Pf6N2E2_4581 0.28 -0.5 5,311,982 - Pf6N2E2_4581 0.28 +0.7 5,312,255 - Pf6N2E2_4581 0.49 +0.6 5,312,330 - Pf6N2E2_4581 0.54 -0.4 5,312,450 - Pf6N2E2_4581 0.63 -0.6 5,312,608 + Pf6N2E2_4581 0.75 +1.3 5,312,608 + Pf6N2E2_4581 0.75 +1.1 5,312,609 - Pf6N2E2_4581 0.75 -0.0 5,312,609 - Pf6N2E2_4581 0.75 -2.3 5,312,609 - Pf6N2E2_4581 0.75 +0.8 5,312,609 - Pf6N2E2_4581 0.75 +0.6 5,312,611 + Pf6N2E2_4581 0.75 -1.5 5,312,611 + Pf6N2E2_4581 0.75 -0.0 5,312,612 - Pf6N2E2_4581 0.75 -1.0 5,312,612 - Pf6N2E2_4581 0.75 -0.4 5,312,761 + Pf6N2E2_4581 0.86 +1.5 5,312,779 + Pf6N2E2_4581 0.87 -2.5 5,312,779 + Pf6N2E2_4581 0.87 -1.1 5,312,779 + Pf6N2E2_4581 0.87 -2.7 5,313,011 + -2.4 5,313,057 + +0.2 5,313,240 + -1.2 5,313,240 + +0.1 5,313,240 + -0.3 5,313,240 + +1.2 5,313,244 + +1.5 5,313,244 + -0.8
Or see this region's nucleotide sequence