Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3136 and Pf6N2E2_3137 are separated by 659 nucleotides Pf6N2E2_3137 and Pf6N2E2_3138 are separated by 158 nucleotides Pf6N2E2_3138 and Pf6N2E2_3139 are separated by 147 nucleotides
Pf6N2E2_3136 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,626,931 to 3,628,037
_3136
Pf6N2E2_3137 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 3,628,697 to 3,630,406
_3137
Pf6N2E2_3138 - YaeQ protein, at 3,630,565 to 3,631,107
_3138
Pf6N2E2_3139 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,631,255 to 3,632,454
_3139
Position (kb)
3628
3629
3630
3631 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.744 kb on + strand, within Pf6N2E2_3136 at 3627.745 kb on - strand, within Pf6N2E2_3136 at 3627.745 kb on - strand, within Pf6N2E2_3136 at 3627.958 kb on + strand at 3628.013 kb on + strand at 3628.015 kb on + strand at 3628.015 kb on + strand at 3628.015 kb on + strand at 3628.015 kb on + strand at 3628.016 kb on - strand at 3628.016 kb on - strand at 3628.016 kb on - strand at 3628.016 kb on - strand at 3628.169 kb on + strand at 3628.177 kb on - strand at 3628.212 kb on + strand at 3628.213 kb on - strand at 3628.770 kb on + strand at 3628.770 kb on + strand at 3628.770 kb on + strand at 3628.771 kb on - strand at 3629.154 kb on + strand, within Pf6N2E2_3137 at 3629.154 kb on + strand, within Pf6N2E2_3137 at 3629.154 kb on + strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.290 kb on - strand, within Pf6N2E2_3137 at 3629.570 kb on - strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.777 kb on - strand, within Pf6N2E2_3137 at 3629.777 kb on - strand, within Pf6N2E2_3137 at 3629.784 kb on + strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3630.001 kb on + strand, within Pf6N2E2_3137 at 3630.002 kb on - strand, within Pf6N2E2_3137 at 3630.008 kb on - strand, within Pf6N2E2_3137 at 3630.081 kb on + strand, within Pf6N2E2_3137 at 3630.081 kb on + strand, within Pf6N2E2_3137 at 3630.082 kb on - strand, within Pf6N2E2_3137 at 3630.082 kb on - strand, within Pf6N2E2_3137 at 3630.105 kb on + strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.330 kb on + strand at 3630.331 kb on - strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.957 kb on - strand, within Pf6N2E2_3138 at 3630.968 kb on + strand, within Pf6N2E2_3138 at 3630.969 kb on - strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.208 kb on - strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.222 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 3,627,744 + Pf6N2E2_3136 0.73 +0.2 3,627,744 + Pf6N2E2_3136 0.73 +0.6 3,627,744 + Pf6N2E2_3136 0.73 -0.7 3,627,744 + Pf6N2E2_3136 0.73 -1.2 3,627,744 + Pf6N2E2_3136 0.73 -1.6 3,627,745 - Pf6N2E2_3136 0.74 +0.1 3,627,745 - Pf6N2E2_3136 0.74 -0.3 3,627,958 + -1.1 3,628,013 + -1.4 3,628,015 + -0.9 3,628,015 + +0.3 3,628,015 + +0.6 3,628,015 + -0.5 3,628,016 - -0.8 3,628,016 - -1.6 3,628,016 - +1.2 3,628,016 - +0.4 3,628,169 + +0.0 3,628,177 - +1.0 3,628,212 + -2.5 3,628,213 - -0.1 3,628,770 + -1.0 3,628,770 + +1.5 3,628,770 + -2.0 3,628,771 - +0.4 3,629,154 + Pf6N2E2_3137 0.27 -2.7 3,629,154 + Pf6N2E2_3137 0.27 -2.7 3,629,154 + Pf6N2E2_3137 0.27 -2.8 3,629,290 - Pf6N2E2_3137 0.35 +0.5 3,629,290 - Pf6N2E2_3137 0.35 +0.5 3,629,290 - Pf6N2E2_3137 0.35 -0.1 3,629,290 - Pf6N2E2_3137 0.35 -0.1 3,629,570 - Pf6N2E2_3137 0.51 +0.5 3,629,776 + Pf6N2E2_3137 0.63 -0.7 3,629,776 + Pf6N2E2_3137 0.63 -0.1 3,629,776 + Pf6N2E2_3137 0.63 -0.2 3,629,777 - Pf6N2E2_3137 0.63 +0.5 3,629,777 - Pf6N2E2_3137 0.63 -0.6 3,629,784 + Pf6N2E2_3137 0.64 -0.6 3,629,785 - Pf6N2E2_3137 0.64 -1.0 3,629,785 - Pf6N2E2_3137 0.64 -0.1 3,629,785 - Pf6N2E2_3137 0.64 -0.2 3,629,785 - Pf6N2E2_3137 0.64 -0.3 3,629,785 - Pf6N2E2_3137 0.64 +0.9 3,629,829 + Pf6N2E2_3137 0.66 -1.1 3,629,829 + Pf6N2E2_3137 0.66 -1.4 3,629,829 + Pf6N2E2_3137 0.66 -0.6 3,629,830 - Pf6N2E2_3137 0.66 -3.5 3,629,830 - Pf6N2E2_3137 0.66 -3.0 3,629,830 - Pf6N2E2_3137 0.66 +0.5 3,629,830 - Pf6N2E2_3137 0.66 -0.4 3,630,001 + Pf6N2E2_3137 0.76 +0.4 3,630,002 - Pf6N2E2_3137 0.76 +0.4 3,630,008 - Pf6N2E2_3137 0.77 -2.6 3,630,081 + Pf6N2E2_3137 0.81 -0.9 3,630,081 + Pf6N2E2_3137 0.81 +0.6 3,630,082 - Pf6N2E2_3137 0.81 -0.1 3,630,082 - Pf6N2E2_3137 0.81 -1.7 3,630,105 + Pf6N2E2_3137 0.82 -1.7 3,630,106 - Pf6N2E2_3137 0.82 +0.2 3,630,106 - Pf6N2E2_3137 0.82 -2.8 3,630,106 - Pf6N2E2_3137 0.82 -0.4 3,630,330 + -2.1 3,630,331 - +0.5 3,630,398 + -1.1 3,630,398 + +0.3 3,630,398 + +0.6 3,630,398 + -1.0 3,630,398 + +0.2 3,630,399 - +0.2 3,630,399 - -0.1 3,630,399 - +0.6 3,630,957 - Pf6N2E2_3138 0.72 -0.3 3,630,968 + Pf6N2E2_3138 0.74 -2.2 3,630,969 - Pf6N2E2_3138 0.74 +0.4 3,631,043 + Pf6N2E2_3138 0.88 +1.9 3,631,043 + Pf6N2E2_3138 0.88 +0.7 3,631,044 - Pf6N2E2_3138 0.88 -2.1 3,631,044 - Pf6N2E2_3138 0.88 +0.6 3,631,044 - Pf6N2E2_3138 0.88 -1.2 3,631,044 - Pf6N2E2_3138 0.88 -1.5 3,631,085 + -0.1 3,631,085 + -0.8 3,631,085 + -0.5 3,631,123 + -0.9 3,631,123 + -0.8 3,631,123 + -1.0 3,631,208 - +0.5 3,631,212 + -0.7 3,631,212 + +0.4 3,631,212 + -2.3 3,631,213 - +0.4 3,631,213 - +2.1 3,631,213 - +0.1 3,631,222 + +0.5
Or see this region's nucleotide sequence