Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2956 and Pf6N2E2_2957 overlap by 4 nucleotides Pf6N2E2_2957 and Pf6N2E2_2958 are separated by 362 nucleotides
Pf6N2E2_2956 - mRNA 3-end processing factor, at 3,446,985 to 3,448,004
_2956
Pf6N2E2_2957 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 3,448,001 to 3,449,689
_2957
Pf6N2E2_2958 - ABC transporter for L-Lysine, periplasmic substrate-binding component (from data) , at 3,450,052 to 3,450,834
_2958
Position (kb)
3448
3449
3450 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3447.005 kb on + strand at 3447.005 kb on + strand at 3447.006 kb on - strand at 3447.006 kb on - strand at 3447.006 kb on - strand at 3447.006 kb on - strand at 3447.027 kb on + strand at 3447.027 kb on + strand at 3447.028 kb on - strand at 3447.028 kb on - strand at 3447.260 kb on - strand, within Pf6N2E2_2956 at 3447.260 kb on - strand, within Pf6N2E2_2956 at 3447.260 kb on - strand, within Pf6N2E2_2956 at 3447.270 kb on + strand, within Pf6N2E2_2956 at 3447.270 kb on + strand, within Pf6N2E2_2956 at 3447.271 kb on - strand, within Pf6N2E2_2956 at 3447.271 kb on - strand, within Pf6N2E2_2956 at 3447.271 kb on - strand, within Pf6N2E2_2956 at 3447.303 kb on + strand, within Pf6N2E2_2956 at 3447.304 kb on - strand, within Pf6N2E2_2956 at 3447.304 kb on - strand, within Pf6N2E2_2956 at 3447.356 kb on + strand, within Pf6N2E2_2956 at 3447.356 kb on + strand, within Pf6N2E2_2956 at 3447.393 kb on + strand, within Pf6N2E2_2956 at 3447.393 kb on + strand, within Pf6N2E2_2956 at 3447.394 kb on - strand, within Pf6N2E2_2956 at 3447.394 kb on - strand, within Pf6N2E2_2956 at 3447.487 kb on - strand, within Pf6N2E2_2956 at 3447.487 kb on - strand, within Pf6N2E2_2956 at 3447.487 kb on - strand, within Pf6N2E2_2956 at 3447.619 kb on - strand, within Pf6N2E2_2956 at 3447.721 kb on - strand, within Pf6N2E2_2956 at 3447.945 kb on + strand at 3447.945 kb on + strand at 3447.946 kb on - strand at 3448.079 kb on + strand at 3448.079 kb on + strand at 3448.079 kb on + strand at 3448.080 kb on - strand at 3448.080 kb on - strand at 3448.080 kb on - strand at 3448.121 kb on + strand at 3448.121 kb on + strand at 3448.122 kb on - strand at 3448.122 kb on - strand at 3448.226 kb on + strand, within Pf6N2E2_2957 at 3448.227 kb on - strand, within Pf6N2E2_2957 at 3448.267 kb on - strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.314 kb on + strand, within Pf6N2E2_2957 at 3448.315 kb on - strand, within Pf6N2E2_2957 at 3448.568 kb on + strand, within Pf6N2E2_2957 at 3448.568 kb on + strand, within Pf6N2E2_2957 at 3449.027 kb on + strand, within Pf6N2E2_2957 at 3449.028 kb on - strand, within Pf6N2E2_2957 at 3449.243 kb on + strand, within Pf6N2E2_2957 at 3449.324 kb on + strand, within Pf6N2E2_2957 at 3449.325 kb on - strand, within Pf6N2E2_2957 at 3449.325 kb on - strand, within Pf6N2E2_2957 at 3449.361 kb on - strand, within Pf6N2E2_2957 at 3449.370 kb on - strand, within Pf6N2E2_2957 at 3449.438 kb on + strand, within Pf6N2E2_2957 at 3449.439 kb on - strand, within Pf6N2E2_2957 at 3449.636 kb on - strand at 3449.656 kb on + strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.657 kb on - strand at 3449.687 kb on + strand at 3449.687 kb on + strand at 3449.687 kb on + strand at 3449.876 kb on + strand at 3449.876 kb on + strand at 3449.876 kb on + strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.877 kb on - strand at 3449.965 kb on - strand at 3449.970 kb on + strand at 3449.970 kb on + strand at 3449.970 kb on + strand at 3449.980 kb on + strand at 3449.981 kb on - strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.007 kb on + strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.008 kb on - strand at 3450.011 kb on + strand at 3450.012 kb on - strand at 3450.012 kb on - strand at 3450.031 kb on - strand at 3450.031 kb on - strand at 3450.039 kb on + strand at 3450.050 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.124 kb on + strand at 3450.125 kb on - strand at 3450.125 kb on - strand at 3450.158 kb on - strand, within Pf6N2E2_2958 at 3450.303 kb on + strand, within Pf6N2E2_2958 at 3450.303 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.397 kb on + strand, within Pf6N2E2_2958 at 3450.398 kb on - strand, within Pf6N2E2_2958 at 3450.398 kb on - strand, within Pf6N2E2_2958 at 3450.490 kb on + strand, within Pf6N2E2_2958 at 3450.490 kb on + strand, within Pf6N2E2_2958 at 3450.491 kb on - strand, within Pf6N2E2_2958 at 3450.556 kb on + strand, within Pf6N2E2_2958 at 3450.649 kb on + strand, within Pf6N2E2_2958
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 3,447,005 + -0.4 3,447,005 + +0.1 3,447,006 - +1.6 3,447,006 - -0.9 3,447,006 - -0.2 3,447,006 - +0.6 3,447,027 + -0.2 3,447,027 + -0.2 3,447,028 - +0.2 3,447,028 - -0.8 3,447,260 - Pf6N2E2_2956 0.27 +1.3 3,447,260 - Pf6N2E2_2956 0.27 +0.3 3,447,260 - Pf6N2E2_2956 0.27 -0.4 3,447,270 + Pf6N2E2_2956 0.28 -2.3 3,447,270 + Pf6N2E2_2956 0.28 +0.2 3,447,271 - Pf6N2E2_2956 0.28 +0.2 3,447,271 - Pf6N2E2_2956 0.28 +0.9 3,447,271 - Pf6N2E2_2956 0.28 -2.2 3,447,303 + Pf6N2E2_2956 0.31 -1.9 3,447,304 - Pf6N2E2_2956 0.31 +0.9 3,447,304 - Pf6N2E2_2956 0.31 -0.8 3,447,356 + Pf6N2E2_2956 0.36 -0.0 3,447,356 + Pf6N2E2_2956 0.36 +1.8 3,447,393 + Pf6N2E2_2956 0.40 +0.1 3,447,393 + Pf6N2E2_2956 0.40 +0.1 3,447,394 - Pf6N2E2_2956 0.40 +0.9 3,447,394 - Pf6N2E2_2956 0.40 -0.3 3,447,487 - Pf6N2E2_2956 0.49 +1.4 3,447,487 - Pf6N2E2_2956 0.49 -2.6 3,447,487 - Pf6N2E2_2956 0.49 +0.3 3,447,619 - Pf6N2E2_2956 0.62 -1.5 3,447,721 - Pf6N2E2_2956 0.72 -0.1 3,447,945 + -2.4 3,447,945 + -3.3 3,447,946 - -0.7 3,448,079 + -1.1 3,448,079 + -1.3 3,448,079 + -2.1 3,448,080 - +0.1 3,448,080 - +0.8 3,448,080 - -0.3 3,448,121 + -1.0 3,448,121 + +0.1 3,448,122 - +0.1 3,448,122 - +2.5 3,448,226 + Pf6N2E2_2957 0.13 +0.1 3,448,227 - Pf6N2E2_2957 0.13 +2.4 3,448,267 - Pf6N2E2_2957 0.16 +1.3 3,448,314 + Pf6N2E2_2957 0.19 +1.7 3,448,314 + Pf6N2E2_2957 0.19 -0.8 3,448,314 + Pf6N2E2_2957 0.19 +0.9 3,448,314 + Pf6N2E2_2957 0.19 +1.8 3,448,315 - Pf6N2E2_2957 0.19 -1.0 3,448,568 + Pf6N2E2_2957 0.34 +1.6 3,448,568 + Pf6N2E2_2957 0.34 +0.1 3,449,027 + Pf6N2E2_2957 0.61 -0.1 3,449,028 - Pf6N2E2_2957 0.61 -0.7 3,449,243 + Pf6N2E2_2957 0.74 +0.2 3,449,324 + Pf6N2E2_2957 0.78 +0.5 3,449,325 - Pf6N2E2_2957 0.78 +1.6 3,449,325 - Pf6N2E2_2957 0.78 +0.3 3,449,361 - Pf6N2E2_2957 0.81 +1.1 3,449,370 - Pf6N2E2_2957 0.81 +1.5 3,449,438 + Pf6N2E2_2957 0.85 +1.0 3,449,439 - Pf6N2E2_2957 0.85 +1.2 3,449,636 - -0.9 3,449,656 + +0.3 3,449,657 - +0.5 3,449,657 - -0.6 3,449,657 - +0.4 3,449,657 - -0.8 3,449,657 - -0.1 3,449,687 + -0.3 3,449,687 + +0.9 3,449,687 + -1.1 3,449,876 + +0.7 3,449,876 + +0.5 3,449,876 + +2.5 3,449,877 - +1.2 3,449,877 - +0.8 3,449,877 - -1.2 3,449,877 - +0.3 3,449,877 - -2.0 3,449,877 - -1.3 3,449,877 - +1.8 3,449,877 - -3.8 3,449,877 - -2.0 3,449,877 - +2.9 3,449,877 - -0.7 3,449,965 - +0.8 3,449,970 + +0.1 3,449,970 + -1.1 3,449,970 + -0.2 3,449,980 + -0.6 3,449,981 - -1.2 3,450,007 + -0.0 3,450,007 + -0.7 3,450,007 + +0.0 3,450,007 + +1.9 3,450,007 + +1.2 3,450,007 + -2.3 3,450,007 + +2.5 3,450,008 - -0.7 3,450,008 - +0.0 3,450,008 - +1.4 3,450,008 - +0.9 3,450,008 - +1.5 3,450,008 - +0.2 3,450,011 + +0.9 3,450,012 - -1.3 3,450,012 - -0.2 3,450,031 - +0.0 3,450,031 - +0.7 3,450,039 + +0.7 3,450,050 + +0.7 3,450,124 + +1.5 3,450,124 + -3.0 3,450,124 + +0.4 3,450,124 + -0.4 3,450,124 + -0.0 3,450,124 + -1.1 3,450,125 - -1.6 3,450,125 - -0.8 3,450,158 - Pf6N2E2_2958 0.14 -0.1 3,450,303 + Pf6N2E2_2958 0.32 +0.7 3,450,303 + Pf6N2E2_2958 0.32 -0.9 3,450,397 + Pf6N2E2_2958 0.44 +0.9 3,450,397 + Pf6N2E2_2958 0.44 -0.1 3,450,397 + Pf6N2E2_2958 0.44 +0.2 3,450,397 + Pf6N2E2_2958 0.44 -0.1 3,450,397 + Pf6N2E2_2958 0.44 +0.6 3,450,397 + Pf6N2E2_2958 0.44 +0.6 3,450,397 + Pf6N2E2_2958 0.44 -1.4 3,450,398 - Pf6N2E2_2958 0.44 +0.8 3,450,398 - Pf6N2E2_2958 0.44 +0.3 3,450,490 + Pf6N2E2_2958 0.56 +0.7 3,450,490 + Pf6N2E2_2958 0.56 +1.8 3,450,491 - Pf6N2E2_2958 0.56 -0.1 3,450,556 + Pf6N2E2_2958 0.64 -1.4 3,450,649 + Pf6N2E2_2958 0.76 -0.7
Or see this region's nucleotide sequence