Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2120 and Pf6N2E2_2121 are separated by 36 nucleotides
Pf6N2E2_2120 - Alpha-amylase (EC 3.2.1.1), at 2,521,164 to 2,523,161
_2120
Pf6N2E2_2121 - hypothetical protein, at 2,523,198 to 2,523,419
_2121
Position (kb)
2523
2524 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2522.285 kb on + strand, within Pf6N2E2_2120 at 2522.286 kb on - strand, within Pf6N2E2_2120 at 2522.321 kb on - strand, within Pf6N2E2_2120 at 2522.410 kb on + strand, within Pf6N2E2_2120 at 2522.410 kb on + strand, within Pf6N2E2_2120 at 2522.411 kb on - strand, within Pf6N2E2_2120 at 2522.411 kb on - strand, within Pf6N2E2_2120 at 2522.518 kb on + strand, within Pf6N2E2_2120 at 2522.519 kb on - strand, within Pf6N2E2_2120 at 2522.584 kb on + strand, within Pf6N2E2_2120 at 2522.585 kb on - strand, within Pf6N2E2_2120 at 2522.585 kb on - strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.854 kb on + strand, within Pf6N2E2_2120 at 2522.855 kb on - strand, within Pf6N2E2_2120 at 2522.855 kb on - strand, within Pf6N2E2_2120 at 2522.855 kb on - strand, within Pf6N2E2_2120 at 2523.086 kb on + strand at 2523.130 kb on + strand at 2523.131 kb on - strand at 2523.131 kb on - strand at 2523.131 kb on - strand at 2523.262 kb on + strand, within Pf6N2E2_2121 at 2523.331 kb on + strand, within Pf6N2E2_2121 at 2523.332 kb on - strand, within Pf6N2E2_2121 at 2523.378 kb on + strand, within Pf6N2E2_2121 at 2523.456 kb on + strand at 2523.456 kb on + strand at 2523.546 kb on + strand at 2523.546 kb on + strand at 2523.546 kb on + strand at 2523.546 kb on + strand at 2523.546 kb on + strand at 2523.546 kb on + strand at 2523.547 kb on - strand at 2523.547 kb on - strand at 2523.633 kb on - strand at 2523.686 kb on + strand at 2523.697 kb on - strand at 2523.781 kb on + strand at 2524.016 kb on + strand at 2524.016 kb on + strand at 2524.016 kb on + strand at 2524.018 kb on + strand at 2524.018 kb on + strand at 2524.093 kb on + strand at 2524.093 kb on + strand at 2524.093 kb on + strand at 2524.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 2,522,285 + Pf6N2E2_2120 0.56 -2.2 2,522,286 - Pf6N2E2_2120 0.56 -1.0 2,522,321 - Pf6N2E2_2120 0.58 -1.0 2,522,410 + Pf6N2E2_2120 0.62 +1.5 2,522,410 + Pf6N2E2_2120 0.62 -1.5 2,522,411 - Pf6N2E2_2120 0.62 +0.4 2,522,411 - Pf6N2E2_2120 0.62 -1.7 2,522,518 + Pf6N2E2_2120 0.68 +0.1 2,522,519 - Pf6N2E2_2120 0.68 -1.8 2,522,584 + Pf6N2E2_2120 0.71 -2.4 2,522,585 - Pf6N2E2_2120 0.71 +0.1 2,522,585 - Pf6N2E2_2120 0.71 -0.8 2,522,854 + Pf6N2E2_2120 0.85 -1.9 2,522,854 + Pf6N2E2_2120 0.85 -0.3 2,522,854 + Pf6N2E2_2120 0.85 -0.4 2,522,854 + Pf6N2E2_2120 0.85 -2.6 2,522,854 + Pf6N2E2_2120 0.85 -0.4 2,522,854 + Pf6N2E2_2120 0.85 +0.6 2,522,854 + Pf6N2E2_2120 0.85 -2.6 2,522,854 + Pf6N2E2_2120 0.85 -0.0 2,522,855 - Pf6N2E2_2120 0.85 +1.5 2,522,855 - Pf6N2E2_2120 0.85 +0.1 2,522,855 - Pf6N2E2_2120 0.85 +0.6 2,523,086 + -0.3 2,523,130 + -2.7 2,523,131 - -0.8 2,523,131 - +0.3 2,523,131 - +2.5 2,523,262 + Pf6N2E2_2121 0.29 -2.1 2,523,331 + Pf6N2E2_2121 0.60 +0.7 2,523,332 - Pf6N2E2_2121 0.60 -1.7 2,523,378 + Pf6N2E2_2121 0.81 -0.3 2,523,456 + +2.3 2,523,456 + -0.4 2,523,546 + +0.4 2,523,546 + +0.6 2,523,546 + -0.6 2,523,546 + +0.8 2,523,546 + -0.3 2,523,546 + -0.5 2,523,547 - +0.4 2,523,547 - +2.2 2,523,633 - -0.2 2,523,686 + +0.1 2,523,697 - +1.0 2,523,781 + -1.0 2,524,016 + -1.4 2,524,016 + +0.2 2,524,016 + +0.5 2,524,018 + +0.7 2,524,018 + +0.3 2,524,093 + -2.4 2,524,093 + -0.2 2,524,093 + -1.0 2,524,093 + -0.7
Or see this region's nucleotide sequence