Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1924 and Pf6N2E2_1925 are separated by 52 nucleotides Pf6N2E2_1925 and Pf6N2E2_1926 overlap by 4 nucleotides Pf6N2E2_1926 and Pf6N2E2_1927 are separated by 421 nucleotides
Pf6N2E2_1924 - 6-phosphogluconolactonase (EC 3.1.1.31), at 2,279,013 to 2,280,185
_1924
Pf6N2E2_1925 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 2,280,238 to 2,280,876
_1925
Pf6N2E2_1926 - hypothetical protein, at 2,280,873 to 2,281,016
_1926
Pf6N2E2_1927 - Capsule biosynthesis protein capA, at 2,281,438 to 2,282,553
_1927
Position (kb)
2280
2281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2279.267 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.303 kb on + strand, within Pf6N2E2_1924 at 2279.388 kb on - strand, within Pf6N2E2_1924 at 2279.436 kb on + strand, within Pf6N2E2_1924 at 2279.457 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.817 kb on + strand, within Pf6N2E2_1924 at 2279.817 kb on + strand, within Pf6N2E2_1924 at 2279.891 kb on - strand, within Pf6N2E2_1924 at 2279.898 kb on + strand, within Pf6N2E2_1924 at 2279.903 kb on + strand, within Pf6N2E2_1924 at 2280.033 kb on + strand, within Pf6N2E2_1924 at 2280.034 kb on - strand, within Pf6N2E2_1924 at 2280.034 kb on - strand, within Pf6N2E2_1924 at 2280.111 kb on + strand at 2280.111 kb on + strand at 2280.112 kb on - strand at 2280.112 kb on - strand at 2280.167 kb on + strand at 2280.167 kb on + strand at 2280.234 kb on - strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.240 kb on - strand at 2280.244 kb on - strand at 2280.488 kb on + strand, within Pf6N2E2_1925 at 2280.489 kb on - strand, within Pf6N2E2_1925 at 2280.544 kb on - strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.552 kb on - strand, within Pf6N2E2_1925 at 2280.560 kb on + strand, within Pf6N2E2_1925 at 2280.561 kb on - strand, within Pf6N2E2_1925 at 2280.566 kb on + strand, within Pf6N2E2_1925 at 2280.566 kb on + strand, within Pf6N2E2_1925 at 2280.567 kb on - strand, within Pf6N2E2_1925 at 2280.570 kb on + strand, within Pf6N2E2_1925 at 2280.634 kb on + strand, within Pf6N2E2_1925 at 2280.634 kb on + strand, within Pf6N2E2_1925 at 2280.645 kb on + strand, within Pf6N2E2_1925 at 2281.006 kb on - strand at 2281.046 kb on + strand at 2281.135 kb on - strand at 2281.329 kb on + strand at 2281.428 kb on - strand at 2281.457 kb on + strand at 2281.520 kb on - strand at 2281.520 kb on - strand at 2281.585 kb on + strand, within Pf6N2E2_1927 at 2281.585 kb on + strand, within Pf6N2E2_1927 at 2281.702 kb on + strand, within Pf6N2E2_1927 at 2281.703 kb on - strand, within Pf6N2E2_1927 at 2281.703 kb on - strand, within Pf6N2E2_1927 at 2281.703 kb on - strand, within Pf6N2E2_1927 at 2281.840 kb on + strand, within Pf6N2E2_1927 at 2281.840 kb on + strand, within Pf6N2E2_1927 at 2281.841 kb on - strand, within Pf6N2E2_1927 at 2281.841 kb on - strand, within Pf6N2E2_1927 at 2281.843 kb on - strand, within Pf6N2E2_1927 at 2281.843 kb on - strand, within Pf6N2E2_1927 at 2281.851 kb on + strand, within Pf6N2E2_1927
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 2,279,267 - Pf6N2E2_1924 0.22 +0.8 2,279,294 - Pf6N2E2_1924 0.24 +2.5 2,279,294 - Pf6N2E2_1924 0.24 +0.4 2,279,303 + Pf6N2E2_1924 0.25 +1.5 2,279,388 - Pf6N2E2_1924 0.32 -0.2 2,279,436 + Pf6N2E2_1924 0.36 -0.9 2,279,457 + Pf6N2E2_1924 0.38 -0.5 2,279,482 + Pf6N2E2_1924 0.40 -0.4 2,279,482 + Pf6N2E2_1924 0.40 +2.1 2,279,682 + Pf6N2E2_1924 0.57 -1.3 2,279,682 + Pf6N2E2_1924 0.57 +1.4 2,279,682 + Pf6N2E2_1924 0.57 +0.2 2,279,683 - Pf6N2E2_1924 0.57 -1.7 2,279,683 - Pf6N2E2_1924 0.57 +0.2 2,279,683 - Pf6N2E2_1924 0.57 -0.4 2,279,683 - Pf6N2E2_1924 0.57 -0.9 2,279,683 - Pf6N2E2_1924 0.57 -2.8 2,279,683 - Pf6N2E2_1924 0.57 -1.0 2,279,817 + Pf6N2E2_1924 0.69 -1.0 2,279,817 + Pf6N2E2_1924 0.69 +0.3 2,279,891 - Pf6N2E2_1924 0.75 -1.4 2,279,898 + Pf6N2E2_1924 0.75 +0.4 2,279,903 + Pf6N2E2_1924 0.76 +1.6 2,280,033 + Pf6N2E2_1924 0.87 -1.2 2,280,034 - Pf6N2E2_1924 0.87 -0.5 2,280,034 - Pf6N2E2_1924 0.87 +1.8 2,280,111 + +2.1 2,280,111 + +0.3 2,280,112 - -1.6 2,280,112 - -0.0 2,280,167 + +0.6 2,280,167 + -2.4 2,280,234 - +1.5 2,280,239 + +0.3 2,280,239 + -3.4 2,280,239 + +1.2 2,280,239 + -2.6 2,280,239 + +1.7 2,280,240 - +0.3 2,280,244 - +1.5 2,280,488 + Pf6N2E2_1925 0.39 -0.0 2,280,489 - Pf6N2E2_1925 0.39 -1.7 2,280,544 - Pf6N2E2_1925 0.48 +0.8 2,280,551 + Pf6N2E2_1925 0.49 -1.4 2,280,551 + Pf6N2E2_1925 0.49 +1.1 2,280,551 + Pf6N2E2_1925 0.49 +1.5 2,280,552 - Pf6N2E2_1925 0.49 -1.1 2,280,560 + Pf6N2E2_1925 0.50 +0.7 2,280,561 - Pf6N2E2_1925 0.51 -1.6 2,280,566 + Pf6N2E2_1925 0.51 +0.0 2,280,566 + Pf6N2E2_1925 0.51 +0.4 2,280,567 - Pf6N2E2_1925 0.51 -0.4 2,280,570 + Pf6N2E2_1925 0.52 -2.5 2,280,634 + Pf6N2E2_1925 0.62 -0.7 2,280,634 + Pf6N2E2_1925 0.62 -3.2 2,280,645 + Pf6N2E2_1925 0.64 -0.1 2,281,006 - -0.4 2,281,046 + +1.1 2,281,135 - -1.3 2,281,329 + -1.2 2,281,428 - -0.2 2,281,457 + +1.6 2,281,520 - +2.1 2,281,520 - +0.5 2,281,585 + Pf6N2E2_1927 0.13 -0.6 2,281,585 + Pf6N2E2_1927 0.13 -0.8 2,281,702 + Pf6N2E2_1927 0.24 +0.9 2,281,703 - Pf6N2E2_1927 0.24 -1.4 2,281,703 - Pf6N2E2_1927 0.24 -0.1 2,281,703 - Pf6N2E2_1927 0.24 +0.9 2,281,840 + Pf6N2E2_1927 0.36 +0.2 2,281,840 + Pf6N2E2_1927 0.36 -0.5 2,281,841 - Pf6N2E2_1927 0.36 -0.3 2,281,841 - Pf6N2E2_1927 0.36 +0.0 2,281,843 - Pf6N2E2_1927 0.36 -2.1 2,281,843 - Pf6N2E2_1927 0.36 +0.9 2,281,851 + Pf6N2E2_1927 0.37 +1.7
Or see this region's nucleotide sequence