Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1923 and Pf6N2E2_1924 are separated by 418 nucleotides Pf6N2E2_1924 and Pf6N2E2_1925 are separated by 52 nucleotides Pf6N2E2_1925 and Pf6N2E2_1926 overlap by 4 nucleotides
Pf6N2E2_1923 - Acetyl-CoA acetyltransferase, at 2,277,425 to 2,278,594
_1923
Pf6N2E2_1924 - 6-phosphogluconolactonase (EC 3.1.1.31), at 2,279,013 to 2,280,185
_1924
Pf6N2E2_1925 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 2,280,238 to 2,280,876
_1925
Pf6N2E2_1926 - hypothetical protein, at 2,280,873 to 2,281,016
_1926
Position (kb)
2279
2280
2281 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2278.079 kb on - strand, within Pf6N2E2_1923 at 2278.131 kb on + strand, within Pf6N2E2_1923 at 2278.188 kb on - strand, within Pf6N2E2_1923 at 2278.188 kb on - strand, within Pf6N2E2_1923 at 2278.268 kb on + strand, within Pf6N2E2_1923 at 2278.269 kb on - strand, within Pf6N2E2_1923 at 2278.296 kb on + strand, within Pf6N2E2_1923 at 2278.296 kb on + strand, within Pf6N2E2_1923 at 2278.417 kb on + strand, within Pf6N2E2_1923 at 2278.663 kb on - strand at 2278.846 kb on - strand at 2278.857 kb on + strand at 2278.857 kb on + strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.858 kb on - strand at 2278.890 kb on - strand at 2279.034 kb on + strand at 2279.035 kb on - strand at 2279.045 kb on - strand at 2279.045 kb on - strand at 2279.064 kb on + strand at 2279.067 kb on - strand at 2279.133 kb on + strand, within Pf6N2E2_1924 at 2279.133 kb on + strand, within Pf6N2E2_1924 at 2279.134 kb on - strand, within Pf6N2E2_1924 at 2279.134 kb on - strand, within Pf6N2E2_1924 at 2279.166 kb on + strand, within Pf6N2E2_1924 at 2279.166 kb on + strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.167 kb on - strand, within Pf6N2E2_1924 at 2279.267 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.294 kb on - strand, within Pf6N2E2_1924 at 2279.303 kb on + strand, within Pf6N2E2_1924 at 2279.388 kb on - strand, within Pf6N2E2_1924 at 2279.436 kb on + strand, within Pf6N2E2_1924 at 2279.457 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.482 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.682 kb on + strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.683 kb on - strand, within Pf6N2E2_1924 at 2279.817 kb on + strand, within Pf6N2E2_1924 at 2279.817 kb on + strand, within Pf6N2E2_1924 at 2279.891 kb on - strand, within Pf6N2E2_1924 at 2279.898 kb on + strand, within Pf6N2E2_1924 at 2279.903 kb on + strand, within Pf6N2E2_1924 at 2280.033 kb on + strand, within Pf6N2E2_1924 at 2280.034 kb on - strand, within Pf6N2E2_1924 at 2280.034 kb on - strand, within Pf6N2E2_1924 at 2280.111 kb on + strand at 2280.111 kb on + strand at 2280.112 kb on - strand at 2280.112 kb on - strand at 2280.167 kb on + strand at 2280.167 kb on + strand at 2280.234 kb on - strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.239 kb on + strand at 2280.240 kb on - strand at 2280.244 kb on - strand at 2280.488 kb on + strand, within Pf6N2E2_1925 at 2280.489 kb on - strand, within Pf6N2E2_1925 at 2280.544 kb on - strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.551 kb on + strand, within Pf6N2E2_1925 at 2280.552 kb on - strand, within Pf6N2E2_1925 at 2280.560 kb on + strand, within Pf6N2E2_1925 at 2280.561 kb on - strand, within Pf6N2E2_1925 at 2280.566 kb on + strand, within Pf6N2E2_1925 at 2280.566 kb on + strand, within Pf6N2E2_1925 at 2280.567 kb on - strand, within Pf6N2E2_1925 at 2280.570 kb on + strand, within Pf6N2E2_1925 at 2280.634 kb on + strand, within Pf6N2E2_1925 at 2280.634 kb on + strand, within Pf6N2E2_1925 at 2280.645 kb on + strand, within Pf6N2E2_1925 at 2281.006 kb on - strand at 2281.046 kb on + strand at 2281.135 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 2,278,079 - Pf6N2E2_1923 0.56 +0.0 2,278,131 + Pf6N2E2_1923 0.60 -1.0 2,278,188 - Pf6N2E2_1923 0.65 -0.4 2,278,188 - Pf6N2E2_1923 0.65 -1.1 2,278,268 + Pf6N2E2_1923 0.72 +0.5 2,278,269 - Pf6N2E2_1923 0.72 +1.8 2,278,296 + Pf6N2E2_1923 0.74 +0.4 2,278,296 + Pf6N2E2_1923 0.74 -0.1 2,278,417 + Pf6N2E2_1923 0.85 -2.0 2,278,663 - -1.5 2,278,846 - +0.9 2,278,857 + -0.7 2,278,857 + -0.9 2,278,858 - +0.3 2,278,858 - +0.9 2,278,858 - +0.4 2,278,858 - -0.8 2,278,890 - +2.2 2,279,034 + +2.8 2,279,035 - +1.2 2,279,045 - +0.4 2,279,045 - +2.2 2,279,064 + +0.4 2,279,067 - +1.0 2,279,133 + Pf6N2E2_1924 0.10 -1.1 2,279,133 + Pf6N2E2_1924 0.10 -2.6 2,279,134 - Pf6N2E2_1924 0.10 -1.8 2,279,134 - Pf6N2E2_1924 0.10 -1.6 2,279,166 + Pf6N2E2_1924 0.13 +0.5 2,279,166 + Pf6N2E2_1924 0.13 +0.8 2,279,167 - Pf6N2E2_1924 0.13 +0.2 2,279,167 - Pf6N2E2_1924 0.13 +0.2 2,279,167 - Pf6N2E2_1924 0.13 -1.6 2,279,267 - Pf6N2E2_1924 0.22 +0.8 2,279,294 - Pf6N2E2_1924 0.24 +2.5 2,279,294 - Pf6N2E2_1924 0.24 +0.4 2,279,303 + Pf6N2E2_1924 0.25 +1.5 2,279,388 - Pf6N2E2_1924 0.32 -0.2 2,279,436 + Pf6N2E2_1924 0.36 -0.9 2,279,457 + Pf6N2E2_1924 0.38 -0.5 2,279,482 + Pf6N2E2_1924 0.40 -0.4 2,279,482 + Pf6N2E2_1924 0.40 +2.1 2,279,682 + Pf6N2E2_1924 0.57 -1.3 2,279,682 + Pf6N2E2_1924 0.57 +1.4 2,279,682 + Pf6N2E2_1924 0.57 +0.2 2,279,683 - Pf6N2E2_1924 0.57 -1.7 2,279,683 - Pf6N2E2_1924 0.57 +0.2 2,279,683 - Pf6N2E2_1924 0.57 -0.4 2,279,683 - Pf6N2E2_1924 0.57 -0.9 2,279,683 - Pf6N2E2_1924 0.57 -2.8 2,279,683 - Pf6N2E2_1924 0.57 -1.0 2,279,817 + Pf6N2E2_1924 0.69 -1.0 2,279,817 + Pf6N2E2_1924 0.69 +0.3 2,279,891 - Pf6N2E2_1924 0.75 -1.4 2,279,898 + Pf6N2E2_1924 0.75 +0.4 2,279,903 + Pf6N2E2_1924 0.76 +1.6 2,280,033 + Pf6N2E2_1924 0.87 -1.2 2,280,034 - Pf6N2E2_1924 0.87 -0.5 2,280,034 - Pf6N2E2_1924 0.87 +1.8 2,280,111 + +2.1 2,280,111 + +0.3 2,280,112 - -1.6 2,280,112 - -0.0 2,280,167 + +0.6 2,280,167 + -2.4 2,280,234 - +1.5 2,280,239 + +0.3 2,280,239 + -3.4 2,280,239 + +1.2 2,280,239 + -2.6 2,280,239 + +1.7 2,280,240 - +0.3 2,280,244 - +1.5 2,280,488 + Pf6N2E2_1925 0.39 -0.0 2,280,489 - Pf6N2E2_1925 0.39 -1.7 2,280,544 - Pf6N2E2_1925 0.48 +0.8 2,280,551 + Pf6N2E2_1925 0.49 -1.4 2,280,551 + Pf6N2E2_1925 0.49 +1.1 2,280,551 + Pf6N2E2_1925 0.49 +1.5 2,280,552 - Pf6N2E2_1925 0.49 -1.1 2,280,560 + Pf6N2E2_1925 0.50 +0.7 2,280,561 - Pf6N2E2_1925 0.51 -1.6 2,280,566 + Pf6N2E2_1925 0.51 +0.0 2,280,566 + Pf6N2E2_1925 0.51 +0.4 2,280,567 - Pf6N2E2_1925 0.51 -0.4 2,280,570 + Pf6N2E2_1925 0.52 -2.5 2,280,634 + Pf6N2E2_1925 0.62 -0.7 2,280,634 + Pf6N2E2_1925 0.62 -3.2 2,280,645 + Pf6N2E2_1925 0.64 -0.1 2,281,006 - -0.4 2,281,046 + +1.1 2,281,135 - -1.3
Or see this region's nucleotide sequence