Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1863

Experiment: Chloride 600 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1861 and Pf6N2E2_1862 are separated by 334 nucleotidesPf6N2E2_1862 and Pf6N2E2_1863 are separated by 0 nucleotidesPf6N2E2_1863 and Pf6N2E2_1864 are separated by 416 nucleotides Pf6N2E2_1861 - Ferredoxin reductase, at 2,204,360 to 2,205,595 _1861 Pf6N2E2_1862 - Benzoate 1,2-dioxygenase beta subunit (EC 1.14.12.10), at 2,205,930 to 2,206,424 _1862 Pf6N2E2_1863 - putative dioxygenase hydroxylase component, at 2,206,425 to 2,207,684 _1863 Pf6N2E2_1864 - Transcriptional regulator, TetR family, at 2,208,101 to 2,208,751 _1864 Position (kb) 2206 2207 2208Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2205.438 kb on + strand, within Pf6N2E2_1861at 2205.439 kb on - strand, within Pf6N2E2_1861at 2205.591 kb on + strandat 2205.592 kb on - strandat 2205.619 kb on + strandat 2205.620 kb on - strandat 2205.701 kb on + strandat 2205.701 kb on + strandat 2205.701 kb on + strandat 2205.702 kb on - strandat 2206.031 kb on - strand, within Pf6N2E2_1862at 2206.092 kb on - strand, within Pf6N2E2_1862at 2206.092 kb on - strand, within Pf6N2E2_1862at 2206.194 kb on - strand, within Pf6N2E2_1862at 2206.237 kb on + strand, within Pf6N2E2_1862at 2206.237 kb on + strand, within Pf6N2E2_1862at 2206.241 kb on + strand, within Pf6N2E2_1862at 2206.295 kb on - strand, within Pf6N2E2_1862at 2206.343 kb on + strand, within Pf6N2E2_1862at 2206.427 kb on - strandat 2206.550 kb on - strandat 2206.597 kb on + strand, within Pf6N2E2_1863at 2206.840 kb on + strand, within Pf6N2E2_1863at 2206.841 kb on - strand, within Pf6N2E2_1863at 2206.865 kb on - strand, within Pf6N2E2_1863at 2206.996 kb on + strand, within Pf6N2E2_1863at 2206.996 kb on + strand, within Pf6N2E2_1863at 2206.997 kb on - strand, within Pf6N2E2_1863at 2206.997 kb on - strand, within Pf6N2E2_1863at 2206.997 kb on - strand, within Pf6N2E2_1863at 2206.997 kb on - strand, within Pf6N2E2_1863at 2206.997 kb on - strand, within Pf6N2E2_1863at 2207.251 kb on + strand, within Pf6N2E2_1863at 2207.369 kb on - strand, within Pf6N2E2_1863at 2207.379 kb on - strand, within Pf6N2E2_1863at 2207.379 kb on - strand, within Pf6N2E2_1863at 2207.811 kb on - strandat 2207.850 kb on - strandat 2207.850 kb on - strandat 2207.988 kb on + strandat 2207.989 kb on - strandat 2207.989 kb on - strandat 2207.989 kb on - strandat 2208.043 kb on + strandat 2208.116 kb on + strandat 2208.176 kb on + strand, within Pf6N2E2_1864at 2208.190 kb on + strand, within Pf6N2E2_1864at 2208.191 kb on - strand, within Pf6N2E2_1864at 2208.191 kb on - strand, within Pf6N2E2_1864at 2208.269 kb on - strand, within Pf6N2E2_1864at 2208.315 kb on + strand, within Pf6N2E2_1864at 2208.564 kb on + strand, within Pf6N2E2_1864at 2208.564 kb on + strand, within Pf6N2E2_1864at 2208.565 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.579 kb on + strand, within Pf6N2E2_1864

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Per-strain Table

Position Strand Gene LocusTag Fraction Chloride 600 mM
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2,205,438 + Pf6N2E2_1861 0.87 +0.0
2,205,439 - Pf6N2E2_1861 0.87 -0.6
2,205,591 + -0.9
2,205,592 - -1.2
2,205,619 + -0.8
2,205,620 - -1.7
2,205,701 + +1.2
2,205,701 + -1.1
2,205,701 + -2.5
2,205,702 - +0.8
2,206,031 - Pf6N2E2_1862 0.20 -3.0
2,206,092 - Pf6N2E2_1862 0.33 -2.4
2,206,092 - Pf6N2E2_1862 0.33 +0.5
2,206,194 - Pf6N2E2_1862 0.53 +1.3
2,206,237 + Pf6N2E2_1862 0.62 -2.3
2,206,237 + Pf6N2E2_1862 0.62 +1.7
2,206,241 + Pf6N2E2_1862 0.63 +0.3
2,206,295 - Pf6N2E2_1862 0.74 -1.7
2,206,343 + Pf6N2E2_1862 0.83 +0.6
2,206,427 - +0.4
2,206,550 - -1.7
2,206,597 + Pf6N2E2_1863 0.14 -0.3
2,206,840 + Pf6N2E2_1863 0.33 +1.3
2,206,841 - Pf6N2E2_1863 0.33 +1.7
2,206,865 - Pf6N2E2_1863 0.35 -0.8
2,206,996 + Pf6N2E2_1863 0.45 -0.8
2,206,996 + Pf6N2E2_1863 0.45 -3.1
2,206,997 - Pf6N2E2_1863 0.45 -1.0
2,206,997 - Pf6N2E2_1863 0.45 -0.4
2,206,997 - Pf6N2E2_1863 0.45 -1.8
2,206,997 - Pf6N2E2_1863 0.45 -2.4
2,206,997 - Pf6N2E2_1863 0.45 -1.6
2,207,251 + Pf6N2E2_1863 0.66 -2.7
2,207,369 - Pf6N2E2_1863 0.75 -0.8
2,207,379 - Pf6N2E2_1863 0.76 -0.8
2,207,379 - Pf6N2E2_1863 0.76 -0.3
2,207,811 - +0.2
2,207,850 - +0.2
2,207,850 - -1.1
2,207,988 + +2.4
2,207,989 - -2.3
2,207,989 - +1.5
2,207,989 - -2.2
2,208,043 + +0.2
2,208,116 + +1.1
2,208,176 + Pf6N2E2_1864 0.12 +0.2
2,208,190 + Pf6N2E2_1864 0.14 +0.6
2,208,191 - Pf6N2E2_1864 0.14 +0.1
2,208,191 - Pf6N2E2_1864 0.14 -2.9
2,208,269 - Pf6N2E2_1864 0.26 -1.3
2,208,315 + Pf6N2E2_1864 0.33 -0.4
2,208,564 + Pf6N2E2_1864 0.71 +0.3
2,208,564 + Pf6N2E2_1864 0.71 -0.1
2,208,565 - Pf6N2E2_1864 0.71 -0.2
2,208,569 - Pf6N2E2_1864 0.72 +0.8
2,208,569 - Pf6N2E2_1864 0.72 -1.8
2,208,569 - Pf6N2E2_1864 0.72 +0.8
2,208,569 - Pf6N2E2_1864 0.72 +0.3
2,208,579 + Pf6N2E2_1864 0.73 +0.4

Or see this region's nucleotide sequence