Experiment: Chloride 600 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_13 and Pf6N2E2_14 overlap by 4 nucleotides Pf6N2E2_14 and Pf6N2E2_15 are separated by 201 nucleotides
Pf6N2E2_13 - Pyoverdin biosynthesis protein PvdN, putative aminotransferase, class V, at 41,933 to 43,213
_13
Pf6N2E2_14 - Putative dipeptidase, pyoverdin biosynthesis PvdM, at 43,210 to 44,580
_14
Pf6N2E2_15 - Pyoverdine biosynthesis related protein PvdP, at 44,782 to 46,380
_15
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 42.360 kb on + strand, within Pf6N2E2_13 at 42.361 kb on - strand, within Pf6N2E2_13 at 42.361 kb on - strand, within Pf6N2E2_13 at 42.507 kb on + strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.508 kb on - strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.822 kb on + strand, within Pf6N2E2_13 at 42.823 kb on - strand, within Pf6N2E2_13 at 42.823 kb on - strand, within Pf6N2E2_13 at 42.971 kb on - strand, within Pf6N2E2_13 at 43.096 kb on + strand at 43.096 kb on + strand at 43.097 kb on - strand at 43.387 kb on + strand, within Pf6N2E2_14 at 43.387 kb on + strand, within Pf6N2E2_14 at 43.387 kb on + strand, within Pf6N2E2_14 at 43.388 kb on - strand, within Pf6N2E2_14 at 43.388 kb on - strand, within Pf6N2E2_14 at 43.455 kb on - strand, within Pf6N2E2_14 at 43.676 kb on + strand, within Pf6N2E2_14 at 43.803 kb on + strand, within Pf6N2E2_14 at 43.991 kb on + strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 43.992 kb on - strand, within Pf6N2E2_14 at 44.135 kb on + strand, within Pf6N2E2_14 at 44.135 kb on + strand, within Pf6N2E2_14 at 44.235 kb on + strand, within Pf6N2E2_14 at 44.236 kb on - strand, within Pf6N2E2_14 at 44.633 kb on - strand at 44.686 kb on + strand at 44.688 kb on + strand at 44.689 kb on - strand at 44.714 kb on + strand at 44.751 kb on + strand at 44.751 kb on + strand at 44.751 kb on + strand at 44.824 kb on + strand at 44.824 kb on + strand at 44.824 kb on + strand at 44.827 kb on + strand at 44.828 kb on - strand at 45.044 kb on - strand, within Pf6N2E2_15 at 45.179 kb on - strand, within Pf6N2E2_15 at 45.179 kb on - strand, within Pf6N2E2_15 at 45.342 kb on + strand, within Pf6N2E2_15 at 45.342 kb on + strand, within Pf6N2E2_15
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 600 mM remove 42,360 + Pf6N2E2_13 0.33 +1.3 42,361 - Pf6N2E2_13 0.33 -3.2 42,361 - Pf6N2E2_13 0.33 -1.5 42,507 + Pf6N2E2_13 0.45 -1.3 42,508 - Pf6N2E2_13 0.45 +0.6 42,508 - Pf6N2E2_13 0.45 -1.2 42,508 - Pf6N2E2_13 0.45 +0.4 42,508 - Pf6N2E2_13 0.45 -1.7 42,822 + Pf6N2E2_13 0.69 -0.5 42,822 + Pf6N2E2_13 0.69 +0.8 42,822 + Pf6N2E2_13 0.69 -0.7 42,822 + Pf6N2E2_13 0.69 -2.3 42,822 + Pf6N2E2_13 0.69 +0.7 42,823 - Pf6N2E2_13 0.69 +0.4 42,823 - Pf6N2E2_13 0.69 +1.2 42,971 - Pf6N2E2_13 0.81 +1.0 43,096 + -0.1 43,096 + -1.2 43,097 - -1.9 43,387 + Pf6N2E2_14 0.13 -2.8 43,387 + Pf6N2E2_14 0.13 +0.6 43,387 + Pf6N2E2_14 0.13 -1.9 43,388 - Pf6N2E2_14 0.13 +0.9 43,388 - Pf6N2E2_14 0.13 +1.6 43,455 - Pf6N2E2_14 0.18 -0.4 43,676 + Pf6N2E2_14 0.34 +0.6 43,803 + Pf6N2E2_14 0.43 -0.1 43,991 + Pf6N2E2_14 0.57 +3.2 43,992 - Pf6N2E2_14 0.57 +0.3 43,992 - Pf6N2E2_14 0.57 +2.3 43,992 - Pf6N2E2_14 0.57 +0.8 44,135 + Pf6N2E2_14 0.67 +1.4 44,135 + Pf6N2E2_14 0.67 +0.6 44,235 + Pf6N2E2_14 0.75 +0.8 44,236 - Pf6N2E2_14 0.75 +1.0 44,633 - -1.0 44,686 + -1.3 44,688 + +0.3 44,689 - +0.9 44,714 + -0.3 44,751 + +0.3 44,751 + -1.6 44,751 + +0.5 44,824 + -2.2 44,824 + +1.0 44,824 + +0.2 44,827 + +1.3 44,828 - -0.2 45,044 - Pf6N2E2_15 0.16 -0.7 45,179 - Pf6N2E2_15 0.25 +2.0 45,179 - Pf6N2E2_15 0.25 +0.2 45,342 + Pf6N2E2_15 0.35 -1.2 45,342 + Pf6N2E2_15 0.35 +1.1
Or see this region's nucleotide sequence