Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00720 and IAI47_00725 are separated by 252 nucleotides IAI47_00725 and IAI47_00730 are separated by 19 nucleotides IAI47_00730 and IAI47_00735 are separated by 38 nucleotides IAI47_00735 and IAI47_00740 overlap by 1 nucleotides
IAI47_00720: IAI47_00720 - murein hydrolase activator EnvC, at 155,232 to 156,533
_00720
IAI47_00725: IAI47_00725 - rhodanese-like domain-containing protein, at 156,786 to 157,217
_00725
IAI47_00730: IAI47_00730 - glutaredoxin 3, at 157,237 to 157,491
_00730
IAI47_00735: IAI47_00735 - protein-export chaperone SecB, at 157,530 to 158,000
_00735
IAI47_00740: IAI47_00740 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 158,000 to 159,016
_00740
Position (kb) 156
157
158 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 155.868 kb on - strand, within IAI47_00720 at 155.922 kb on + strand, within IAI47_00720 at 155.923 kb on - strand, within IAI47_00720 at 155.973 kb on + strand, within IAI47_00720 at 155.973 kb on - strand, within IAI47_00720 at 155.974 kb on - strand, within IAI47_00720 at 155.974 kb on - strand, within IAI47_00720 at 155.974 kb on - strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.029 kb on + strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.030 kb on - strand, within IAI47_00720 at 156.032 kb on - strand, within IAI47_00720 at 156.032 kb on - strand, within IAI47_00720 at 156.032 kb on - strand, within IAI47_00720 at 156.032 kb on - strand, within IAI47_00720 at 156.032 kb on - strand, within IAI47_00720 at 156.032 kb on - strand, within IAI47_00720 at 156.041 kb on - strand, within IAI47_00720 at 156.050 kb on + strand, within IAI47_00720 at 156.050 kb on + strand, within IAI47_00720 at 156.050 kb on + strand, within IAI47_00720 at 156.051 kb on - strand, within IAI47_00720 at 156.051 kb on - strand, within IAI47_00720 at 156.051 kb on - strand, within IAI47_00720 at 156.051 kb on - strand, within IAI47_00720 at 156.051 kb on - strand, within IAI47_00720 at 156.093 kb on + strand, within IAI47_00720 at 156.093 kb on + strand, within IAI47_00720 at 156.093 kb on + strand, within IAI47_00720 at 156.093 kb on + strand, within IAI47_00720 at 156.093 kb on + strand, within IAI47_00720 at 156.107 kb on + strand, within IAI47_00720 at 156.108 kb on - strand, within IAI47_00720 at 156.212 kb on + strand, within IAI47_00720 at 156.213 kb on - strand, within IAI47_00720 at 156.231 kb on - strand, within IAI47_00720 at 156.300 kb on + strand, within IAI47_00720 at 156.300 kb on + strand, within IAI47_00720 at 156.300 kb on + strand, within IAI47_00720 at 156.300 kb on + strand, within IAI47_00720 at 156.300 kb on + strand, within IAI47_00720 at 156.301 kb on - strand, within IAI47_00720 at 156.417 kb on + strand at 156.417 kb on + strand at 156.417 kb on + strand at 156.418 kb on - strand at 156.418 kb on - strand at 156.443 kb on + strand at 156.444 kb on - strand at 156.465 kb on + strand at 156.465 kb on + strand at 156.465 kb on + strand at 156.465 kb on + strand at 156.465 kb on + strand at 156.466 kb on - strand at 156.466 kb on - strand at 156.466 kb on - strand at 156.466 kb on - strand at 156.466 kb on - strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.533 kb on + strand at 156.534 kb on - strand at 156.534 kb on - strand at 156.534 kb on - strand at 156.534 kb on - strand at 156.545 kb on + strand at 156.545 kb on + strand at 156.545 kb on + strand at 156.546 kb on - strand at 156.546 kb on - strand at 156.546 kb on - strand at 156.567 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.602 kb on + strand at 156.603 kb on - strand at 156.603 kb on - strand at 156.603 kb on - strand at 156.603 kb on - strand at 156.608 kb on + strand at 156.608 kb on + strand at 156.609 kb on - strand at 156.609 kb on - strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.624 kb on + strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.625 kb on - strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.629 kb on + strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.630 kb on - strand at 156.637 kb on - strand at 156.651 kb on - strand at 156.689 kb on + strand at 156.689 kb on + strand at 156.702 kb on + strand at 156.702 kb on + strand at 156.702 kb on + strand at 156.703 kb on - strand at 156.703 kb on - strand at 156.703 kb on - strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.711 kb on + strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.712 kb on - strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.731 kb on + strand at 156.732 kb on - strand at 156.732 kb on - strand at 156.733 kb on + strand at 156.751 kb on + strand at 156.751 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.756 kb on + strand at 156.759 kb on + strand at 156.759 kb on + strand at 156.797 kb on + strand at 156.890 kb on + strand, within IAI47_00725 at 156.890 kb on + strand, within IAI47_00725 at 156.891 kb on - strand, within IAI47_00725 at 156.949 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 156.955 kb on + strand, within IAI47_00725 at 157.028 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.075 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.084 kb on + strand, within IAI47_00725 at 157.169 kb on + strand, within IAI47_00725 at 157.169 kb on + strand, within IAI47_00725 at 157.169 kb on + strand, within IAI47_00725 at 157.201 kb on + strand at 157.201 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.215 kb on + strand at 157.221 kb on + strand at 157.221 kb on + strand at 157.221 kb on + strand at 157.221 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.255 kb on + strand at 157.257 kb on + strand at 157.257 kb on + strand at 157.257 kb on + strand at 157.257 kb on + strand at 157.276 kb on + strand, within IAI47_00730 at 157.276 kb on + strand, within IAI47_00730 at 157.316 kb on + strand, within IAI47_00730 at 157.335 kb on + strand, within IAI47_00730 at 157.386 kb on + strand, within IAI47_00730 at 157.442 kb on + strand, within IAI47_00730 at 157.442 kb on + strand, within IAI47_00730 at 157.442 kb on + strand, within IAI47_00730 at 157.469 kb on + strand at 157.469 kb on + strand at 157.469 kb on + strand at 157.478 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.512 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.516 kb on + strand at 157.541 kb on + strand at 157.541 kb on + strand at 157.541 kb on + strand at 157.541 kb on + strand at 157.649 kb on + strand, within IAI47_00735 at 157.695 kb on + strand, within IAI47_00735 at 157.720 kb on + strand, within IAI47_00735 at 157.830 kb on + strand, within IAI47_00735 at 157.892 kb on + strand, within IAI47_00735
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 155,868 - IAI47_00720 0.49 +1.5 155,922 + IAI47_00720 0.53 +1.3 155,923 - IAI47_00720 0.53 -0.8 155,973 + IAI47_00720 0.57 -1.4 155,973 - IAI47_00720 0.57 +0.9 155,974 - IAI47_00720 0.57 -0.6 155,974 - IAI47_00720 0.57 -0.6 155,974 - IAI47_00720 0.57 -1.4 156,029 + IAI47_00720 0.61 -2.6 156,029 + IAI47_00720 0.61 -0.7 156,029 + IAI47_00720 0.61 -3.0 156,029 + IAI47_00720 0.61 -1.7 156,029 + IAI47_00720 0.61 -0.8 156,029 + IAI47_00720 0.61 -1.6 156,029 + IAI47_00720 0.61 -2.3 156,029 + IAI47_00720 0.61 -1.1 156,029 + IAI47_00720 0.61 -0.8 156,029 + IAI47_00720 0.61 -1.9 156,029 + IAI47_00720 0.61 -0.8 156,029 + IAI47_00720 0.61 -2.1 156,029 + IAI47_00720 0.61 -1.6 156,030 - IAI47_00720 0.61 +0.2 156,030 - IAI47_00720 0.61 -1.0 156,030 - IAI47_00720 0.61 -1.7 156,030 - IAI47_00720 0.61 +2.0 156,030 - IAI47_00720 0.61 +0.2 156,030 - IAI47_00720 0.61 -0.3 156,030 - IAI47_00720 0.61 +0.0 156,030 - IAI47_00720 0.61 +0.3 156,030 - IAI47_00720 0.61 -0.1 156,030 - IAI47_00720 0.61 -1.2 156,030 - IAI47_00720 0.61 +0.9 156,030 - IAI47_00720 0.61 -1.2 156,032 - IAI47_00720 0.61 -1.6 156,032 - IAI47_00720 0.61 -0.9 156,032 - IAI47_00720 0.61 -0.2 156,032 - IAI47_00720 0.61 -1.2 156,032 - IAI47_00720 0.61 +0.8 156,032 - IAI47_00720 0.61 -0.7 156,041 - IAI47_00720 0.62 -0.8 156,050 + IAI47_00720 0.63 -2.6 156,050 + IAI47_00720 0.63 -2.6 156,050 + IAI47_00720 0.63 -1.9 156,051 - IAI47_00720 0.63 +1.2 156,051 - IAI47_00720 0.63 -0.7 156,051 - IAI47_00720 0.63 -0.2 156,051 - IAI47_00720 0.63 -0.9 156,051 - IAI47_00720 0.63 -0.6 156,093 + IAI47_00720 0.66 -3.5 156,093 + IAI47_00720 0.66 -0.2 156,093 + IAI47_00720 0.66 -0.7 156,093 + IAI47_00720 0.66 -0.7 156,093 + IAI47_00720 0.66 +0.2 156,107 + IAI47_00720 0.67 -1.0 156,108 - IAI47_00720 0.67 -0.9 156,212 + IAI47_00720 0.75 -2.2 156,213 - IAI47_00720 0.75 -1.0 156,231 - IAI47_00720 0.77 +0.8 156,300 + IAI47_00720 0.82 -2.2 156,300 + IAI47_00720 0.82 -2.2 156,300 + IAI47_00720 0.82 -0.5 156,300 + IAI47_00720 0.82 -0.8 156,300 + IAI47_00720 0.82 -1.8 156,301 - IAI47_00720 0.82 -2.1 156,417 + -1.3 156,417 + -1.8 156,417 + -1.5 156,418 - +0.5 156,418 - -0.8 156,443 + -1.5 156,444 - -2.2 156,465 + +0.1 156,465 + -2.5 156,465 + -1.5 156,465 + -0.0 156,465 + -0.8 156,466 - -0.4 156,466 - +0.8 156,466 - -3.5 156,466 - +1.5 156,466 - -0.6 156,533 + +0.5 156,533 + +0.8 156,533 + -0.0 156,533 + -2.0 156,533 + -0.9 156,533 + -0.3 156,533 + -1.5 156,534 - +1.2 156,534 - +0.7 156,534 - -1.2 156,534 - -0.1 156,545 + -1.3 156,545 + -1.0 156,545 + -1.7 156,546 - -0.4 156,546 - -1.3 156,546 - -0.3 156,567 + -0.6 156,602 + -0.6 156,602 + +1.2 156,602 + +0.2 156,602 + +1.1 156,602 + -1.5 156,602 + -0.3 156,602 + -1.3 156,603 - -1.5 156,603 - -0.4 156,603 - +0.2 156,603 - -1.9 156,608 + +0.9 156,608 + +0.8 156,609 - -0.7 156,609 - -0.3 156,624 + -0.4 156,624 + -0.7 156,624 + -1.8 156,624 + +0.3 156,624 + +0.4 156,624 + -1.3 156,624 + +0.8 156,624 + +1.0 156,624 + +0.3 156,624 + -0.0 156,624 + +1.3 156,624 + -1.5 156,624 + -1.8 156,624 + +2.4 156,624 + +0.6 156,624 + -0.9 156,625 - -0.2 156,625 - -0.4 156,625 - +2.8 156,625 - +3.1 156,625 - -1.5 156,625 - +0.0 156,625 - +0.4 156,625 - -1.5 156,625 - +3.3 156,625 - +0.2 156,625 - -0.4 156,625 - -0.1 156,625 - +1.1 156,625 - -0.9 156,625 - -0.8 156,629 + -1.5 156,629 + -0.6 156,629 + -1.5 156,629 + -1.0 156,629 + -2.0 156,630 - +0.3 156,630 - +2.5 156,630 - -2.8 156,630 - -0.3 156,630 - +0.5 156,630 - +0.3 156,637 - +0.2 156,651 - -1.0 156,689 + +2.1 156,689 + -0.6 156,702 + -0.0 156,702 + -0.8 156,702 + +0.3 156,703 - +0.4 156,703 - +1.6 156,703 - +1.0 156,711 + -0.8 156,711 + -0.3 156,711 + +0.1 156,711 + +2.0 156,711 + +1.3 156,711 + +1.1 156,711 + -1.6 156,711 + -0.6 156,712 - +0.3 156,712 - +1.1 156,712 - -0.0 156,712 - -0.3 156,712 - -0.4 156,712 - +1.1 156,731 + -1.4 156,731 + +2.8 156,731 + -0.5 156,731 + +0.1 156,731 + -1.1 156,732 - +0.8 156,732 - +0.6 156,733 + +1.0 156,751 + -0.2 156,751 + +3.4 156,756 + +0.3 156,756 + -0.7 156,756 + +3.6 156,756 + +0.8 156,756 + +1.3 156,756 + -0.2 156,756 + +0.3 156,756 + -1.0 156,759 + -0.2 156,759 + -0.1 156,797 + -0.2 156,890 + IAI47_00725 0.24 +2.1 156,890 + IAI47_00725 0.24 +1.6 156,891 - IAI47_00725 0.24 -0.8 156,949 + IAI47_00725 0.38 +0.9 156,955 + IAI47_00725 0.39 -0.1 156,955 + IAI47_00725 0.39 +1.4 156,955 + IAI47_00725 0.39 -0.4 156,955 + IAI47_00725 0.39 +1.1 157,028 + IAI47_00725 0.56 +1.1 157,075 + IAI47_00725 0.67 +0.0 157,075 + IAI47_00725 0.67 +0.4 157,075 + IAI47_00725 0.67 +1.4 157,075 + IAI47_00725 0.67 +1.5 157,075 + IAI47_00725 0.67 +2.1 157,075 + IAI47_00725 0.67 -0.3 157,084 + IAI47_00725 0.69 -0.0 157,084 + IAI47_00725 0.69 -1.5 157,084 + IAI47_00725 0.69 +1.7 157,084 + IAI47_00725 0.69 -0.5 157,169 + IAI47_00725 0.89 +0.2 157,169 + IAI47_00725 0.89 +0.3 157,169 + IAI47_00725 0.89 +0.0 157,201 + +1.3 157,201 + -0.0 157,215 + +0.2 157,215 + -0.1 157,215 + +0.7 157,215 + +2.7 157,215 + +1.1 157,215 + +1.5 157,215 + +1.0 157,215 + -1.4 157,215 + -1.4 157,215 + +2.3 157,215 + +1.2 157,215 + -0.7 157,215 + +2.4 157,221 + -0.9 157,221 + +2.5 157,221 + -0.5 157,221 + +1.1 157,255 + +4.9 157,255 + +2.3 157,255 + -1.5 157,255 + -1.1 157,255 + +0.5 157,255 + -1.3 157,255 + +4.1 157,257 + +3.0 157,257 + +1.0 157,257 + -0.8 157,257 + +1.0 157,276 + IAI47_00730 0.15 -0.4 157,276 + IAI47_00730 0.15 +0.5 157,316 + IAI47_00730 0.31 +1.1 157,335 + IAI47_00730 0.38 -1.1 157,386 + IAI47_00730 0.58 -0.9 157,442 + IAI47_00730 0.80 +3.4 157,442 + IAI47_00730 0.80 -1.2 157,442 + IAI47_00730 0.80 +1.1 157,469 + -0.5 157,469 + -2.3 157,469 + -0.1 157,478 + -0.4 157,512 + +3.2 157,512 + +2.6 157,512 + +1.0 157,512 + +1.3 157,512 + +3.6 157,512 + +2.4 157,512 + +0.3 157,512 + +0.4 157,512 + +2.2 157,512 + +1.7 157,512 + +0.3 157,512 + +1.2 157,512 + -0.0 157,516 + +0.4 157,516 + +0.2 157,516 + -1.2 157,516 + +1.4 157,516 + +0.4 157,516 + +2.1 157,516 + +0.8 157,516 + +1.9 157,516 + +2.0 157,516 + -0.2 157,516 + -0.4 157,516 + +1.9 157,516 + +0.3 157,516 + +0.1 157,516 + +3.4 157,516 + +1.5 157,516 + +1.5 157,516 + +1.5 157,541 + +3.1 157,541 + +0.5 157,541 + +0.1 157,541 + +0.2 157,649 + IAI47_00735 0.25 -0.9 157,695 + IAI47_00735 0.35 -0.7 157,720 + IAI47_00735 0.40 +1.1 157,830 + IAI47_00735 0.64 +4.1 157,892 + IAI47_00735 0.77 -1.2
Or see this region's nucleotide sequence