Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21035 and BWI76_RS21040 are separated by 81 nucleotides BWI76_RS21040 and BWI76_RS21045 overlap by 4 nucleotides BWI76_RS21045 and BWI76_RS21050 overlap by 4 nucleotides BWI76_RS21050 and BWI76_RS21055 are separated by 119 nucleotides
BWI76_RS21035: BWI76_RS21035 - exodeoxyribonuclease VII large subunit, at 4,402,303 to 4,403,676
_RS21035
BWI76_RS21040: BWI76_RS21040 - peptidase M4, at 4,403,758 to 4,404,786
_RS21040
BWI76_RS21045: BWI76_RS21045 - hypothetical protein, at 4,404,783 to 4,405,127
_RS21045
BWI76_RS21050: BWI76_RS21050 - hypothetical protein, at 4,405,124 to 4,405,339
_RS21050
BWI76_RS21055: BWI76_RS21055 - Tol-Pal system TolA, at 4,405,459 to 4,405,851
_RS21055
Position (kb)
4403
4404
4405 Strain fitness (log2 ratio)
-1
0
1
2 at 4402.833 kb on + strand, within BWI76_RS21035 at 4402.833 kb on + strand, within BWI76_RS21035 at 4402.833 kb on + strand, within BWI76_RS21035 at 4402.841 kb on - strand, within BWI76_RS21035 at 4402.841 kb on - strand, within BWI76_RS21035 at 4402.865 kb on + strand, within BWI76_RS21035 at 4402.993 kb on - strand, within BWI76_RS21035 at 4403.434 kb on + strand, within BWI76_RS21035 at 4403.830 kb on - strand at 4403.858 kb on - strand at 4403.978 kb on + strand, within BWI76_RS21040 at 4404.006 kb on - strand, within BWI76_RS21040 at 4404.042 kb on - strand, within BWI76_RS21040 at 4404.267 kb on + strand, within BWI76_RS21040 at 4404.390 kb on - strand, within BWI76_RS21040 at 4404.450 kb on + strand, within BWI76_RS21040 at 4404.768 kb on + strand at 4405.098 kb on + strand at 4405.185 kb on - strand, within BWI76_RS21050 at 4405.244 kb on + strand, within BWI76_RS21050 at 4405.252 kb on - strand, within BWI76_RS21050 at 4405.252 kb on - strand, within BWI76_RS21050 at 4405.292 kb on + strand, within BWI76_RS21050 at 4405.292 kb on + strand, within BWI76_RS21050 at 4405.300 kb on - strand, within BWI76_RS21050 at 4405.304 kb on + strand, within BWI76_RS21050 at 4405.309 kb on + strand, within BWI76_RS21050 at 4405.389 kb on + strand at 4405.391 kb on - strand at 4405.408 kb on + strand at 4405.413 kb on + strand at 4405.416 kb on - strand at 4405.416 kb on - strand at 4405.444 kb on - strand at 4405.483 kb on + strand at 4405.483 kb on + strand at 4405.486 kb on - strand at 4405.507 kb on - strand, within BWI76_RS21055 at 4405.509 kb on + strand, within BWI76_RS21055 at 4405.524 kb on + strand, within BWI76_RS21055 at 4405.552 kb on + strand, within BWI76_RS21055 at 4405.555 kb on - strand, within BWI76_RS21055 at 4405.590 kb on + strand, within BWI76_RS21055 at 4405.595 kb on + strand, within BWI76_RS21055 at 4405.600 kb on + strand, within BWI76_RS21055 at 4405.604 kb on + strand, within BWI76_RS21055 at 4405.608 kb on - strand, within BWI76_RS21055 at 4405.612 kb on - strand, within BWI76_RS21055 at 4405.672 kb on - strand, within BWI76_RS21055 at 4405.700 kb on + strand, within BWI76_RS21055 at 4405.708 kb on - strand, within BWI76_RS21055 at 4405.753 kb on + strand, within BWI76_RS21055 at 4405.761 kb on - strand, within BWI76_RS21055 at 4405.769 kb on - strand, within BWI76_RS21055
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 4,402,833 + BWI76_RS21035 0.39 -1.7 4,402,833 + BWI76_RS21035 0.39 -0.6 4,402,833 + BWI76_RS21035 0.39 +0.3 4,402,841 - BWI76_RS21035 0.39 -0.7 4,402,841 - BWI76_RS21035 0.39 -0.2 4,402,865 + BWI76_RS21035 0.41 -1.4 4,402,993 - BWI76_RS21035 0.50 -0.1 4,403,434 + BWI76_RS21035 0.82 -0.4 4,403,830 - -0.2 4,403,858 - +0.5 4,403,978 + BWI76_RS21040 0.21 +0.0 4,404,006 - BWI76_RS21040 0.24 +0.7 4,404,042 - BWI76_RS21040 0.28 -0.1 4,404,267 + BWI76_RS21040 0.49 +0.9 4,404,390 - BWI76_RS21040 0.61 +0.5 4,404,450 + BWI76_RS21040 0.67 -1.1 4,404,768 + +0.1 4,405,098 + +0.0 4,405,185 - BWI76_RS21050 0.28 -0.0 4,405,244 + BWI76_RS21050 0.56 -0.4 4,405,252 - BWI76_RS21050 0.59 +0.0 4,405,252 - BWI76_RS21050 0.59 -0.2 4,405,292 + BWI76_RS21050 0.78 -0.1 4,405,292 + BWI76_RS21050 0.78 +0.5 4,405,300 - BWI76_RS21050 0.81 +1.7 4,405,304 + BWI76_RS21050 0.83 +0.1 4,405,309 + BWI76_RS21050 0.86 -0.4 4,405,389 + +1.1 4,405,391 - +1.2 4,405,408 + +2.1 4,405,413 + -0.2 4,405,416 - -0.5 4,405,416 - -0.2 4,405,444 - +0.5 4,405,483 + -1.1 4,405,483 + +0.4 4,405,486 - -0.1 4,405,507 - BWI76_RS21055 0.12 +0.2 4,405,509 + BWI76_RS21055 0.13 -0.4 4,405,524 + BWI76_RS21055 0.17 +2.1 4,405,552 + BWI76_RS21055 0.24 -0.6 4,405,555 - BWI76_RS21055 0.24 +0.3 4,405,590 + BWI76_RS21055 0.33 +0.5 4,405,595 + BWI76_RS21055 0.35 +0.2 4,405,600 + BWI76_RS21055 0.36 -0.4 4,405,604 + BWI76_RS21055 0.37 -0.2 4,405,608 - BWI76_RS21055 0.38 -1.6 4,405,612 - BWI76_RS21055 0.39 -0.9 4,405,672 - BWI76_RS21055 0.54 -0.5 4,405,700 + BWI76_RS21055 0.61 -0.1 4,405,708 - BWI76_RS21055 0.63 -0.3 4,405,753 + BWI76_RS21055 0.75 -0.8 4,405,761 - BWI76_RS21055 0.77 +1.0 4,405,769 - BWI76_RS21055 0.79 +0.2
Or see this region's nucleotide sequence