Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15925 and BWI76_RS15930 are separated by 10 nucleotides BWI76_RS15930 and BWI76_RS15935 are separated by 1 nucleotides BWI76_RS15935 and BWI76_RS15940 are separated by 41 nucleotides
BWI76_RS15925: BWI76_RS15925 - dimethyl sulfoxide reductase subunit A, at 3,302,259 to 3,304,694
_RS15925
BWI76_RS15930: BWI76_RS15930 - dimethylsulfoxide reductase, chain B, at 3,304,705 to 3,305,322
_RS15930
BWI76_RS15935: BWI76_RS15935 - dimethyl sulfoxide reductase, at 3,305,324 to 3,306,181
_RS15935
BWI76_RS15940: BWI76_RS15940 - DMSO reductase maturation protein DsmD, at 3,306,223 to 3,306,831
_RS15940
Position (kb)
3304
3305
3306 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3303.716 kb on + strand, within BWI76_RS15925 at 3303.748 kb on - strand, within BWI76_RS15925 at 3304.008 kb on - strand, within BWI76_RS15925 at 3304.663 kb on + strand at 3304.928 kb on + strand, within BWI76_RS15930 at 3304.928 kb on + strand, within BWI76_RS15930 at 3304.936 kb on - strand, within BWI76_RS15930 at 3304.936 kb on - strand, within BWI76_RS15930 at 3305.042 kb on + strand, within BWI76_RS15930 at 3305.723 kb on + strand, within BWI76_RS15935
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,303,716 + BWI76_RS15925 0.60 -0.8 3,303,748 - BWI76_RS15925 0.61 -2.1 3,304,008 - BWI76_RS15925 0.72 +0.6 3,304,663 + -0.6 3,304,928 + BWI76_RS15930 0.36 +0.0 3,304,928 + BWI76_RS15930 0.36 +0.7 3,304,936 - BWI76_RS15930 0.37 -1.1 3,304,936 - BWI76_RS15930 0.37 +2.3 3,305,042 + BWI76_RS15930 0.55 -1.1 3,305,723 + BWI76_RS15935 0.47 +0.7
Or see this region's nucleotide sequence