Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15280 and BWI76_RS15285 are separated by 73 nucleotides BWI76_RS15285 and BWI76_RS15290 are separated by 261 nucleotides BWI76_RS15290 and BWI76_RS15295 are separated by 17 nucleotides
BWI76_RS15280: BWI76_RS15280 - sugar transporter, at 3,202,034 to 3,203,233
_RS15280
BWI76_RS15285: BWI76_RS15285 - stress protection protein MarC, at 3,203,307 to 3,203,975
_RS15285
BWI76_RS15290: BWI76_RS15290 - transcriptional regulator, at 3,204,237 to 3,204,671
_RS15290
BWI76_RS15295: BWI76_RS15295 - MDR efflux pump AcrAB transcriptional activator MarA, at 3,204,689 to 3,205,069
_RS15295
Position (kb)
3203
3204 Strain fitness (log2 ratio)
-1
0
1
2 at 3202.605 kb on - strand, within BWI76_RS15280 at 3202.883 kb on + strand, within BWI76_RS15280 at 3203.038 kb on + strand, within BWI76_RS15280 at 3203.159 kb on + strand at 3203.446 kb on - strand, within BWI76_RS15285 at 3203.473 kb on - strand, within BWI76_RS15285 at 3203.478 kb on - strand, within BWI76_RS15285 at 3203.863 kb on + strand, within BWI76_RS15285 at 3203.942 kb on + strand at 3203.950 kb on - strand at 3204.060 kb on - strand at 3204.104 kb on + strand at 3204.104 kb on + strand at 3204.122 kb on - strand at 3204.393 kb on + strand, within BWI76_RS15290 at 3204.632 kb on + strand at 3204.875 kb on - strand, within BWI76_RS15295 at 3204.875 kb on - strand, within BWI76_RS15295 at 3204.917 kb on + strand, within BWI76_RS15295 at 3204.918 kb on + strand, within BWI76_RS15295 at 3204.926 kb on - strand, within BWI76_RS15295
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 3,202,605 - BWI76_RS15280 0.48 +0.0 3,202,883 + BWI76_RS15280 0.71 -0.5 3,203,038 + BWI76_RS15280 0.84 -0.2 3,203,159 + +0.2 3,203,446 - BWI76_RS15285 0.21 -0.3 3,203,473 - BWI76_RS15285 0.25 +0.9 3,203,478 - BWI76_RS15285 0.26 +0.6 3,203,863 + BWI76_RS15285 0.83 +0.0 3,203,942 + +0.7 3,203,950 - +1.8 3,204,060 - +0.3 3,204,104 + +0.2 3,204,104 + +0.3 3,204,122 - -1.7 3,204,393 + BWI76_RS15290 0.36 -0.4 3,204,632 + -0.4 3,204,875 - BWI76_RS15295 0.49 -0.9 3,204,875 - BWI76_RS15295 0.49 +0.2 3,204,917 + BWI76_RS15295 0.60 +0.2 3,204,918 + BWI76_RS15295 0.60 -0.7 3,204,926 - BWI76_RS15295 0.62 +0.1
Or see this region's nucleotide sequence