Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13505 and BWI76_RS13510 are separated by 171 nucleotides BWI76_RS13510 and BWI76_RS13515 are separated by 49 nucleotides BWI76_RS13515 and BWI76_RS13520 are separated by 267 nucleotides
BWI76_RS13505: BWI76_RS13505 - putative receptor, at 2,815,979 to 2,817,040
_RS13505
BWI76_RS13510: BWI76_RS13510 - YgdI/YgdR family lipoprotein, at 2,817,212 to 2,817,433
_RS13510
BWI76_RS13515: BWI76_RS13515 - amino acid permease, at 2,817,483 to 2,818,940
_RS13515
BWI76_RS13520: BWI76_RS13520 - hypothetical protein, at 2,819,208 to 2,819,747
_RS13520
Position (kb)
2817
2818
2819 Strain fitness (log2 ratio)
-2
-1
0
1 at 2816.490 kb on + strand, within BWI76_RS13505 at 2816.516 kb on + strand, within BWI76_RS13505 at 2816.838 kb on + strand at 2816.894 kb on - strand, within BWI76_RS13505 at 2816.938 kb on - strand at 2817.004 kb on + strand at 2817.049 kb on + strand at 2817.161 kb on - strand at 2817.188 kb on - strand at 2817.800 kb on - strand, within BWI76_RS13515 at 2817.956 kb on - strand, within BWI76_RS13515 at 2817.964 kb on + strand, within BWI76_RS13515 at 2818.049 kb on + strand, within BWI76_RS13515 at 2818.928 kb on + strand at 2819.035 kb on + strand at 2819.764 kb on + strand at 2819.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,816,490 + BWI76_RS13505 0.48 +0.2 2,816,516 + BWI76_RS13505 0.51 +1.4 2,816,838 + +0.7 2,816,894 - BWI76_RS13505 0.86 +0.7 2,816,938 - -0.3 2,817,004 + +0.0 2,817,049 + +0.0 2,817,161 - +0.1 2,817,188 - -0.3 2,817,800 - BWI76_RS13515 0.22 +0.9 2,817,956 - BWI76_RS13515 0.32 +0.9 2,817,964 + BWI76_RS13515 0.33 -0.4 2,818,049 + BWI76_RS13515 0.39 +0.6 2,818,928 + -0.7 2,819,035 + -2.6 2,819,764 + -0.1 2,819,772 - +0.6
Or see this region's nucleotide sequence