Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10695 and BWI76_RS10700 are separated by 245 nucleotides BWI76_RS10700 and BWI76_RS10705 are separated by 25 nucleotides
BWI76_RS10695: BWI76_RS10695 - aldehyde dehydrogenase PuuC, at 2,260,130 to 2,261,617
_RS10695
BWI76_RS10700: BWI76_RS10700 - XRE family transcriptional regulator, at 2,261,863 to 2,262,420
_RS10700
BWI76_RS10705: BWI76_RS10705 - Gamma-glutamyl-gamma-aminobutyrate hydrolase (EC 3.5.1.94) (from data) , at 2,262,446 to 2,263,210
_RS10705
Position (kb)
2261
2262
2263 Strain fitness (log2 ratio)
-1
0
1 at 2261.044 kb on + strand, within BWI76_RS10695 at 2261.160 kb on + strand, within BWI76_RS10695 at 2261.206 kb on - strand, within BWI76_RS10695 at 2261.317 kb on + strand, within BWI76_RS10695 at 2261.374 kb on + strand, within BWI76_RS10695 at 2261.431 kb on - strand, within BWI76_RS10695 at 2261.470 kb on + strand at 2261.516 kb on + strand at 2261.696 kb on + strand at 2262.144 kb on - strand, within BWI76_RS10700 at 2262.196 kb on - strand, within BWI76_RS10700 at 2262.321 kb on + strand, within BWI76_RS10700 at 2262.329 kb on - strand, within BWI76_RS10700 at 2262.473 kb on + strand at 2262.543 kb on + strand, within BWI76_RS10705 at 2262.563 kb on + strand, within BWI76_RS10705 at 2262.639 kb on - strand, within BWI76_RS10705 at 2262.796 kb on - strand, within BWI76_RS10705 at 2262.916 kb on + strand, within BWI76_RS10705 at 2262.928 kb on + strand, within BWI76_RS10705 at 2263.134 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 2,261,044 + BWI76_RS10695 0.61 -0.2 2,261,160 + BWI76_RS10695 0.69 -0.6 2,261,206 - BWI76_RS10695 0.72 -0.7 2,261,317 + BWI76_RS10695 0.80 -0.2 2,261,374 + BWI76_RS10695 0.84 -0.2 2,261,431 - BWI76_RS10695 0.87 +0.0 2,261,470 + +0.2 2,261,516 + +0.5 2,261,696 + +0.1 2,262,144 - BWI76_RS10700 0.50 -1.0 2,262,196 - BWI76_RS10700 0.60 +0.5 2,262,321 + BWI76_RS10700 0.82 +0.5 2,262,329 - BWI76_RS10700 0.84 +0.6 2,262,473 + +0.5 2,262,543 + BWI76_RS10705 0.13 +0.2 2,262,563 + BWI76_RS10705 0.15 -0.2 2,262,639 - BWI76_RS10705 0.25 +0.4 2,262,796 - BWI76_RS10705 0.46 -0.8 2,262,916 + BWI76_RS10705 0.61 -0.1 2,262,928 + BWI76_RS10705 0.63 -0.0 2,263,134 + -0.2
Or see this region's nucleotide sequence