Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09450 and BWI76_RS09455 are separated by 89 nucleotides BWI76_RS09455 and BWI76_RS09460 are separated by 349 nucleotides
BWI76_RS09450: BWI76_RS09450 - 30S ribosomal protein S6--L-glutamate ligase, at 1,978,755 to 1,979,657
_RS09450
BWI76_RS09455: BWI76_RS09455 - YbjN domain-containing protein, at 1,979,747 to 1,980,226
_RS09455
BWI76_RS09460: BWI76_RS09460 - spermidine/putrescine ABC transporter substrate-binding protein PotF, at 1,980,576 to 1,981,688
_RS09460
Position (kb)
1979
1980
1981 Strain fitness (log2 ratio)
-2
-1
0
1 at 1978.787 kb on + strand at 1979.000 kb on + strand, within BWI76_RS09450 at 1979.534 kb on + strand, within BWI76_RS09450 at 1979.932 kb on + strand, within BWI76_RS09455 at 1980.089 kb on - strand, within BWI76_RS09455 at 1980.110 kb on - strand, within BWI76_RS09455 at 1980.153 kb on - strand, within BWI76_RS09455 at 1980.174 kb on - strand, within BWI76_RS09455 at 1980.403 kb on - strand at 1980.419 kb on + strand at 1980.606 kb on + strand at 1980.611 kb on + strand at 1980.611 kb on + strand at 1980.619 kb on - strand at 1980.619 kb on - strand at 1980.675 kb on - strand at 1980.790 kb on - strand, within BWI76_RS09460 at 1980.816 kb on - strand, within BWI76_RS09460 at 1980.864 kb on - strand, within BWI76_RS09460 at 1981.028 kb on + strand, within BWI76_RS09460 at 1981.036 kb on - strand, within BWI76_RS09460
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,978,787 + +0.7 1,979,000 + BWI76_RS09450 0.27 +0.8 1,979,534 + BWI76_RS09450 0.86 -0.6 1,979,932 + BWI76_RS09455 0.39 +0.2 1,980,089 - BWI76_RS09455 0.71 +0.6 1,980,110 - BWI76_RS09455 0.76 +0.5 1,980,153 - BWI76_RS09455 0.85 +1.1 1,980,174 - BWI76_RS09455 0.89 +1.2 1,980,403 - -0.3 1,980,419 + -0.9 1,980,606 + -0.2 1,980,611 + -0.5 1,980,611 + -0.2 1,980,619 - -0.3 1,980,619 - -0.1 1,980,675 - -0.3 1,980,790 - BWI76_RS09460 0.19 +0.4 1,980,816 - BWI76_RS09460 0.22 -0.8 1,980,864 - BWI76_RS09460 0.26 -0.5 1,981,028 + BWI76_RS09460 0.41 +0.5 1,981,036 - BWI76_RS09460 0.41 -1.8
Or see this region's nucleotide sequence