Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07195 and BWI76_RS07200 are separated by 59 nucleotides BWI76_RS07200 and BWI76_RS07205 are separated by 267 nucleotides
BWI76_RS07195: BWI76_RS07195 - lysine decarboxylase CadA, at 1,511,627 to 1,513,774
_RS07195
BWI76_RS07200: BWI76_RS07200 - MFS transporter, at 1,513,834 to 1,515,318
_RS07200
BWI76_RS07205: BWI76_RS07205 - lysine--tRNA ligase, at 1,515,586 to 1,517,103
_RS07205
Position (kb)
1513
1514
1515
1516 Strain fitness (log2 ratio)
-1
0
1
2 at 1513.573 kb on - strand at 1513.592 kb on - strand at 1513.721 kb on - strand at 1513.740 kb on - strand at 1513.829 kb on - strand at 1513.952 kb on + strand at 1513.980 kb on + strand at 1513.988 kb on - strand, within BWI76_RS07200 at 1513.994 kb on + strand, within BWI76_RS07200 at 1514.104 kb on + strand, within BWI76_RS07200 at 1514.186 kb on - strand, within BWI76_RS07200 at 1514.282 kb on - strand, within BWI76_RS07200 at 1514.332 kb on + strand, within BWI76_RS07200 at 1514.521 kb on - strand, within BWI76_RS07200 at 1514.623 kb on - strand, within BWI76_RS07200 at 1514.803 kb on + strand, within BWI76_RS07200 at 1514.811 kb on - strand, within BWI76_RS07200 at 1514.869 kb on + strand, within BWI76_RS07200 at 1514.972 kb on + strand, within BWI76_RS07200 at 1515.205 kb on + strand at 1515.270 kb on - strand at 1515.313 kb on + strand at 1515.358 kb on + strand at 1515.465 kb on - strand at 1515.465 kb on - strand at 1515.465 kb on - strand at 1515.498 kb on + strand at 1515.498 kb on + strand at 1515.541 kb on - strand at 1515.681 kb on + strand at 1515.681 kb on + strand at 1515.719 kb on + strand at 1515.727 kb on - strand at 1515.727 kb on - strand at 1515.729 kb on + strand at 1515.739 kb on + strand, within BWI76_RS07205 at 1515.977 kb on - strand, within BWI76_RS07205 at 1516.141 kb on - strand, within BWI76_RS07205 at 1516.173 kb on + strand, within BWI76_RS07205 at 1516.181 kb on - strand, within BWI76_RS07205
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,513,573 - -0.8 1,513,592 - +0.1 1,513,721 - +0.3 1,513,740 - -0.2 1,513,829 - +0.2 1,513,952 + -0.1 1,513,980 + +0.7 1,513,988 - BWI76_RS07200 0.10 +0.9 1,513,994 + BWI76_RS07200 0.11 +0.1 1,514,104 + BWI76_RS07200 0.18 -0.0 1,514,186 - BWI76_RS07200 0.24 -0.1 1,514,282 - BWI76_RS07200 0.30 +0.1 1,514,332 + BWI76_RS07200 0.34 +1.3 1,514,521 - BWI76_RS07200 0.46 -0.9 1,514,623 - BWI76_RS07200 0.53 +1.0 1,514,803 + BWI76_RS07200 0.65 +0.3 1,514,811 - BWI76_RS07200 0.66 +1.8 1,514,869 + BWI76_RS07200 0.70 +0.4 1,514,972 + BWI76_RS07200 0.77 +0.1 1,515,205 + -0.5 1,515,270 - +0.2 1,515,313 + -0.1 1,515,358 + +2.5 1,515,465 - -0.1 1,515,465 - -0.7 1,515,465 - +0.4 1,515,498 + +0.4 1,515,498 + -0.3 1,515,541 - -0.0 1,515,681 + +0.1 1,515,681 + +0.1 1,515,719 + +0.0 1,515,727 - +0.7 1,515,727 - -0.2 1,515,729 + -0.5 1,515,739 + BWI76_RS07205 0.10 +0.4 1,515,977 - BWI76_RS07205 0.26 -0.5 1,516,141 - BWI76_RS07205 0.37 +2.2 1,516,173 + BWI76_RS07205 0.39 -0.3 1,516,181 - BWI76_RS07205 0.39 +0.3
Or see this region's nucleotide sequence