Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05325 and BWI76_RS05330 overlap by 4 nucleotides BWI76_RS05330 and BWI76_RS05335 are separated by 20 nucleotides BWI76_RS05335 and BWI76_RS05340 are separated by 64 nucleotides
BWI76_RS05325: BWI76_RS05325 - hypothetical protein, at 1,122,340 to 1,122,885
_RS05325
BWI76_RS05330: BWI76_RS05330 - aminoacyl-tRNA hydrolase, at 1,122,882 to 1,123,295
_RS05330
BWI76_RS05335: BWI76_RS05335 - copper homeostasis/adhesion lipoprotein NlpE, at 1,123,316 to 1,124,035
_RS05335
BWI76_RS05340: BWI76_RS05340 - proline--tRNA ligase, at 1,124,100 to 1,125,818
_RS05340
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-1
0
1 at 1122.463 kb on - strand, within BWI76_RS05325 at 1122.595 kb on - strand, within BWI76_RS05325 at 1122.743 kb on - strand, within BWI76_RS05325 at 1122.754 kb on - strand, within BWI76_RS05325 at 1122.762 kb on + strand, within BWI76_RS05325 at 1122.813 kb on - strand, within BWI76_RS05325 at 1122.813 kb on - strand, within BWI76_RS05325 at 1123.014 kb on + strand, within BWI76_RS05330 at 1123.167 kb on - strand, within BWI76_RS05330 at 1123.214 kb on - strand, within BWI76_RS05330 at 1123.296 kb on + strand at 1123.569 kb on + strand, within BWI76_RS05335 at 1123.875 kb on - strand, within BWI76_RS05335 at 1124.023 kb on - strand at 1124.058 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 1,122,463 - BWI76_RS05325 0.23 -0.2 1,122,595 - BWI76_RS05325 0.47 +1.0 1,122,743 - BWI76_RS05325 0.74 +1.4 1,122,754 - BWI76_RS05325 0.76 -0.0 1,122,762 + BWI76_RS05325 0.77 -0.2 1,122,813 - BWI76_RS05325 0.87 +0.6 1,122,813 - BWI76_RS05325 0.87 +0.4 1,123,014 + BWI76_RS05330 0.32 -0.3 1,123,167 - BWI76_RS05330 0.69 -0.3 1,123,214 - BWI76_RS05330 0.80 -0.0 1,123,296 + -0.4 1,123,569 + BWI76_RS05335 0.35 +0.1 1,123,875 - BWI76_RS05335 0.78 +0.7 1,124,023 - -0.3 1,124,058 + -0.1
Or see this region's nucleotide sequence