Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04085 and BWI76_RS04090 overlap by 32 nucleotides BWI76_RS04090 and BWI76_RS04095 are separated by 14 nucleotides BWI76_RS04095 and BWI76_RS04100 are separated by 91 nucleotides
BWI76_RS04085: BWI76_RS04085 - DNA polymerase III subunit psi, at 841,445 to 841,858
_RS04085
BWI76_RS04090: BWI76_RS04090 - ribosomal-protein-alanine N-acetyltransferase RimI, at 841,827 to 842,273
_RS04090
BWI76_RS04095: BWI76_RS04095 - dUMP phosphatase, at 842,288 to 842,965
_RS04095
BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646
_RS04100
Position (kb) 842
843 Strain fitness (log2 ratio) -1
0
1 at 841.857 kb on + strand at 841.983 kb on + strand, within BWI76_RS04090 at 842.066 kb on + strand, within BWI76_RS04090 at 842.076 kb on - strand, within BWI76_RS04090 at 842.128 kb on + strand, within BWI76_RS04090 at 842.143 kb on + strand, within BWI76_RS04090 at 842.730 kb on - strand, within BWI76_RS04095 at 842.876 kb on - strand, within BWI76_RS04095 at 843.799 kb on - strand, within BWI76_RS04100 at 843.868 kb on + strand, within BWI76_RS04100
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 841,857 + -0.0 841,983 + BWI76_RS04090 0.35 -0.2 842,066 + BWI76_RS04090 0.53 +0.1 842,076 - BWI76_RS04090 0.56 -1.0 842,128 + BWI76_RS04090 0.67 +0.8 842,143 + BWI76_RS04090 0.71 +0.0 842,730 - BWI76_RS04095 0.65 +0.4 842,876 - BWI76_RS04095 0.87 +0.7 843,799 - BWI76_RS04100 0.47 -1.2 843,868 + BWI76_RS04100 0.51 -0.8
Or see this region's nucleotide sequence