Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02700 and BWI76_RS02705 are separated by 115 nucleotides BWI76_RS02705 and BWI76_RS02710 are separated by 82 nucleotides BWI76_RS02710 and BWI76_RS02715 are separated by 75 nucleotides BWI76_RS02715 and BWI76_RS02720 are separated by 80 nucleotides
BWI76_RS02700: BWI76_RS02700 - D-serine/D-alanine/glycine transporter, at 546,870 to 548,273
_RS02700
BWI76_RS02705: BWI76_RS02705 - iron-sulfur cluster repair di-iron protein, at 548,389 to 549,051
_RS02705
BWI76_RS02710: BWI76_RS02710 - EamA family transporter, at 549,134 to 550,105
_RS02710
BWI76_RS02715: BWI76_RS02715 - putative helix-turn-helix AraC-type transcriptional regulator, at 550,181 to 551,005
_RS02715
BWI76_RS02720: BWI76_RS02720 - NAD(P)-dependent oxidoreductase, at 551,086 to 551,934
_RS02720
Position (kb) 549
550
551 Strain fitness (log2 ratio) -1
0
1 at 548.215 kb on + strand at 548.218 kb on - strand at 548.258 kb on - strand at 548.408 kb on + strand at 548.408 kb on + strand at 548.519 kb on + strand, within BWI76_RS02705 at 548.554 kb on - strand, within BWI76_RS02705 at 548.615 kb on + strand, within BWI76_RS02705 at 548.615 kb on + strand, within BWI76_RS02705 at 548.684 kb on - strand, within BWI76_RS02705 at 548.701 kb on - strand, within BWI76_RS02705 at 549.182 kb on + strand at 549.280 kb on - strand, within BWI76_RS02710 at 549.395 kb on + strand, within BWI76_RS02710 at 549.408 kb on - strand, within BWI76_RS02710 at 549.432 kb on - strand, within BWI76_RS02710 at 549.573 kb on + strand, within BWI76_RS02710 at 549.583 kb on + strand, within BWI76_RS02710 at 549.591 kb on - strand, within BWI76_RS02710 at 549.591 kb on - strand, within BWI76_RS02710 at 549.602 kb on - strand, within BWI76_RS02710 at 549.711 kb on + strand, within BWI76_RS02710 at 549.716 kb on + strand, within BWI76_RS02710 at 549.857 kb on + strand, within BWI76_RS02710 at 549.857 kb on + strand, within BWI76_RS02710 at 549.857 kb on + strand, within BWI76_RS02710 at 549.959 kb on + strand, within BWI76_RS02710 at 550.138 kb on + strand at 550.138 kb on + strand at 550.224 kb on - strand at 550.235 kb on + strand at 550.247 kb on + strand at 550.255 kb on - strand at 550.255 kb on - strand at 550.521 kb on - strand, within BWI76_RS02715 at 550.654 kb on + strand, within BWI76_RS02715 at 550.761 kb on + strand, within BWI76_RS02715 at 550.789 kb on + strand, within BWI76_RS02715 at 550.818 kb on - strand, within BWI76_RS02715 at 550.881 kb on - strand, within BWI76_RS02715 at 550.896 kb on - strand, within BWI76_RS02715 at 550.896 kb on - strand, within BWI76_RS02715 at 550.985 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 548,215 + +0.8 548,218 - +0.9 548,258 - -0.2 548,408 + +0.1 548,408 + +0.8 548,519 + BWI76_RS02705 0.20 -0.4 548,554 - BWI76_RS02705 0.25 -0.8 548,615 + BWI76_RS02705 0.34 -0.1 548,615 + BWI76_RS02705 0.34 -0.3 548,684 - BWI76_RS02705 0.44 -0.6 548,701 - BWI76_RS02705 0.47 -0.6 549,182 + -0.2 549,280 - BWI76_RS02710 0.15 +0.2 549,395 + BWI76_RS02710 0.27 +0.3 549,408 - BWI76_RS02710 0.28 -0.4 549,432 - BWI76_RS02710 0.31 -0.6 549,573 + BWI76_RS02710 0.45 +0.4 549,583 + BWI76_RS02710 0.46 +0.3 549,591 - BWI76_RS02710 0.47 -0.6 549,591 - BWI76_RS02710 0.47 +0.3 549,602 - BWI76_RS02710 0.48 +1.4 549,711 + BWI76_RS02710 0.59 +0.1 549,716 + BWI76_RS02710 0.60 +0.4 549,857 + BWI76_RS02710 0.74 +0.4 549,857 + BWI76_RS02710 0.74 +1.6 549,857 + BWI76_RS02710 0.74 +1.4 549,959 + BWI76_RS02710 0.85 +0.4 550,138 + -0.2 550,138 + +0.3 550,224 - -1.1 550,235 + -0.3 550,247 + +0.3 550,255 - -0.1 550,255 - -0.4 550,521 - BWI76_RS02715 0.41 +1.4 550,654 + BWI76_RS02715 0.57 -0.2 550,761 + BWI76_RS02715 0.70 +0.2 550,789 + BWI76_RS02715 0.74 +0.5 550,818 - BWI76_RS02715 0.77 +0.7 550,881 - BWI76_RS02715 0.85 -0.0 550,896 - BWI76_RS02715 0.87 +0.5 550,896 - BWI76_RS02715 0.87 -0.7 550,985 - +0.1
Or see this region's nucleotide sequence