Experiment: Fusidic 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01245 and BWI76_RS01250 are separated by 61 nucleotides BWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides BWI76_RS01255 and BWI76_RS01260 are separated by 109 nucleotides
BWI76_RS01245: BWI76_RS01245 - DNA helicase RecQ, at 254,595 to 256,421
_RS01245
BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106
_RS01250
BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973
_RS01255
BWI76_RS01260: BWI76_RS01260 - lysophospholipase L2, at 258,083 to 259,075
_RS01260
Position (kb) 256
257
258 Strain fitness (log2 ratio) -2
-1
0
1 at 255.654 kb on - strand, within BWI76_RS01245 at 255.695 kb on + strand, within BWI76_RS01245 at 255.739 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.885 kb on + strand, within BWI76_RS01245 at 255.963 kb on + strand, within BWI76_RS01245 at 256.006 kb on + strand, within BWI76_RS01245 at 256.258 kb on + strand at 256.357 kb on - strand at 256.376 kb on - strand at 256.408 kb on + strand at 256.535 kb on + strand at 256.543 kb on - strand at 256.633 kb on - strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.643 kb on + strand, within BWI76_RS01250 at 256.651 kb on - strand, within BWI76_RS01250 at 256.656 kb on - strand, within BWI76_RS01250 at 256.718 kb on + strand, within BWI76_RS01250 at 256.807 kb on - strand, within BWI76_RS01250 at 256.875 kb on - strand, within BWI76_RS01250 at 257.129 kb on + strand at 257.137 kb on - strand at 257.179 kb on + strand at 257.379 kb on - strand at 257.379 kb on - strand at 257.464 kb on - strand, within BWI76_RS01255 at 257.533 kb on + strand, within BWI76_RS01255 at 257.543 kb on + strand, within BWI76_RS01255 at 257.551 kb on - strand, within BWI76_RS01255 at 257.687 kb on - strand, within BWI76_RS01255 at 257.689 kb on + strand, within BWI76_RS01255 at 257.696 kb on + strand, within BWI76_RS01255 at 257.761 kb on + strand, within BWI76_RS01255 at 257.769 kb on - strand, within BWI76_RS01255 at 257.811 kb on + strand, within BWI76_RS01255 at 257.853 kb on - strand at 258.014 kb on - strand at 258.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.5 mg/ml remove 255,654 - BWI76_RS01245 0.58 -0.2 255,695 + BWI76_RS01245 0.60 -0.3 255,739 + BWI76_RS01245 0.63 +0.4 255,885 + BWI76_RS01245 0.71 -0.8 255,885 + BWI76_RS01245 0.71 +0.6 255,885 + BWI76_RS01245 0.71 +0.1 255,963 + BWI76_RS01245 0.75 -0.2 256,006 + BWI76_RS01245 0.77 +1.5 256,258 + -0.1 256,357 - -0.1 256,376 - -0.0 256,408 + -0.6 256,535 + -0.0 256,543 - +0.1 256,633 - BWI76_RS01250 0.24 -2.0 256,643 + BWI76_RS01250 0.26 +0.0 256,643 + BWI76_RS01250 0.26 -0.5 256,651 - BWI76_RS01250 0.27 -0.8 256,656 - BWI76_RS01250 0.28 -0.3 256,718 + BWI76_RS01250 0.38 -0.9 256,807 - BWI76_RS01250 0.52 -0.8 256,875 - BWI76_RS01250 0.63 -0.2 257,129 + +1.3 257,137 - +0.5 257,179 + +0.6 257,379 - +0.2 257,379 - +0.0 257,464 - BWI76_RS01255 0.18 -0.4 257,533 + BWI76_RS01255 0.29 +0.1 257,543 + BWI76_RS01255 0.31 +0.2 257,551 - BWI76_RS01255 0.32 +0.3 257,687 - BWI76_RS01255 0.54 +0.2 257,689 + BWI76_RS01255 0.54 +0.1 257,696 + BWI76_RS01255 0.55 -0.1 257,761 + BWI76_RS01255 0.66 -0.3 257,769 - BWI76_RS01255 0.67 +0.5 257,811 + BWI76_RS01255 0.74 +0.6 257,853 - +0.2 258,014 - -0.7 258,080 - -0.7
Or see this region's nucleotide sequence