Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3998 and BT3999 are separated by 72 nucleotides BT3999 and BT4000 are separated by 33 nucleotides BT4000 and BT4001 are separated by 0 nucleotides
BT3998: BT3998 - GTP pyrophosphokinase (NCBI ptt file), at 5,209,459 to 5,211,702
BT3998
BT3999: BT3999 - membrane-bound lytic murein transglycosylase D presursor (NCBI ptt file), at 5,211,775 to 5,213,070
BT3999
BT4000: BT4000 - hypothetical protein (NCBI ptt file), at 5,213,104 to 5,213,991
BT4000
BT4001: BT4001 - putative chromosome partitioning protein parB (NCBI ptt file), at 5,213,992 to 5,214,882
BT4001
Position (kb) 5211
5212
5213
5214 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5211.776 kb on + strand at 5211.842 kb on - strand at 5211.844 kb on - strand at 5211.856 kb on + strand at 5211.967 kb on - strand, within BT3999 at 5212.032 kb on + strand, within BT3999 at 5212.078 kb on + strand, within BT3999 at 5212.083 kb on + strand, within BT3999 at 5212.197 kb on + strand, within BT3999 at 5212.213 kb on - strand, within BT3999 at 5212.265 kb on + strand, within BT3999 at 5212.389 kb on + strand, within BT3999 at 5212.417 kb on + strand, within BT3999 at 5212.440 kb on - strand, within BT3999 at 5212.443 kb on + strand, within BT3999 at 5212.464 kb on - strand, within BT3999 at 5212.511 kb on - strand, within BT3999 at 5212.632 kb on - strand, within BT3999 at 5212.680 kb on + strand, within BT3999 at 5212.681 kb on - strand, within BT3999 at 5212.681 kb on - strand, within BT3999 at 5212.681 kb on - strand, within BT3999 at 5212.689 kb on - strand, within BT3999 at 5212.742 kb on + strand, within BT3999 at 5212.743 kb on - strand, within BT3999 at 5212.747 kb on - strand, within BT3999 at 5212.773 kb on + strand, within BT3999 at 5212.774 kb on - strand, within BT3999 at 5212.776 kb on - strand, within BT3999 at 5212.857 kb on + strand, within BT3999 at 5212.883 kb on + strand, within BT3999 at 5212.932 kb on + strand, within BT3999 at 5212.933 kb on - strand, within BT3999 at 5212.984 kb on - strand at 5212.988 kb on - strand at 5213.081 kb on + strand at 5213.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 5,211,776 + -3.3 5,211,842 - -1.3 5,211,844 - -0.5 5,211,856 + +0.5 5,211,967 - BT3999 0.15 +0.0 5,212,032 + BT3999 0.20 -1.1 5,212,078 + BT3999 0.23 -2.5 5,212,083 + BT3999 0.24 +1.0 5,212,197 + BT3999 0.33 -2.6 5,212,213 - BT3999 0.34 -1.1 5,212,265 + BT3999 0.38 -0.5 5,212,389 + BT3999 0.47 -3.4 5,212,417 + BT3999 0.50 -3.0 5,212,440 - BT3999 0.51 -1.3 5,212,443 + BT3999 0.52 +0.0 5,212,464 - BT3999 0.53 -3.0 5,212,511 - BT3999 0.57 -0.5 5,212,632 - BT3999 0.66 -1.8 5,212,680 + BT3999 0.70 -1.3 5,212,681 - BT3999 0.70 +2.0 5,212,681 - BT3999 0.70 -1.9 5,212,681 - BT3999 0.70 -2.2 5,212,689 - BT3999 0.71 +0.5 5,212,742 + BT3999 0.75 -3.4 5,212,743 - BT3999 0.75 -4.1 5,212,747 - BT3999 0.75 -2.7 5,212,773 + BT3999 0.77 -3.3 5,212,774 - BT3999 0.77 -2.4 5,212,776 - BT3999 0.77 -2.6 5,212,857 + BT3999 0.83 +1.0 5,212,883 + BT3999 0.85 -2.3 5,212,932 + BT3999 0.89 -0.5 5,212,933 - BT3999 0.89 +0.0 5,212,984 - -1.1 5,212,988 - -2.4 5,213,081 + -3.5 5,213,093 - -2.8
Or see this region's nucleotide sequence