Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3932 and BT3933 are separated by 114 nucleotides BT3933 and BT3934 are separated by 216 nucleotides
BT3932: BT3932 - DNA primase (NCBI ptt file), at 5,104,729 to 5,106,921
BT3932
BT3933: BT3933 - prephenate dehydrogenase (EC 1.3.1.13) (from data) , at 5,107,036 to 5,107,809
BT3933
BT3934: BT3934 - putative phospho-2-dehydro-3-deoxyheptonate aldolase/chorismate mutase (NCBI ptt file), at 5,108,026 to 5,109,087
BT3934
Position (kb)
5107
5108 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 5106.956 kb on - strand at 5106.970 kb on - strand at 5107.010 kb on + strand at 5107.038 kb on - strand at 5107.344 kb on + strand, within BT3933 at 5107.345 kb on - strand, within BT3933 at 5107.345 kb on - strand, within BT3933 at 5107.346 kb on + strand, within BT3933 at 5107.425 kb on + strand, within BT3933 at 5107.480 kb on - strand, within BT3933 at 5107.480 kb on - strand, within BT3933 at 5107.518 kb on - strand, within BT3933 at 5107.588 kb on + strand, within BT3933 at 5107.588 kb on + strand, within BT3933 at 5107.588 kb on + strand, within BT3933 at 5107.589 kb on - strand, within BT3933 at 5107.685 kb on + strand, within BT3933 at 5107.694 kb on + strand, within BT3933 at 5107.694 kb on + strand, within BT3933 at 5107.756 kb on + strand at 5107.757 kb on - strand at 5107.798 kb on + strand at 5107.802 kb on - strand at 5107.810 kb on - strand at 5107.813 kb on + strand at 5107.814 kb on - strand at 5107.844 kb on + strand at 5107.844 kb on + strand at 5107.935 kb on - strand at 5107.943 kb on - strand at 5108.037 kb on + strand at 5108.037 kb on + strand at 5108.037 kb on + strand at 5108.037 kb on + strand at 5108.038 kb on - strand at 5108.110 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 5,106,956 - +1.1 5,106,970 - -1.2 5,107,010 + -1.7 5,107,038 - -0.7 5,107,344 + BT3933 0.40 -6.7 5,107,345 - BT3933 0.40 +0.1 5,107,345 - BT3933 0.40 -0.5 5,107,346 + BT3933 0.40 -1.5 5,107,425 + BT3933 0.50 -1.7 5,107,480 - BT3933 0.57 -1.9 5,107,480 - BT3933 0.57 -5.1 5,107,518 - BT3933 0.62 -4.1 5,107,588 + BT3933 0.71 -1.2 5,107,588 + BT3933 0.71 -2.9 5,107,588 + BT3933 0.71 -3.3 5,107,589 - BT3933 0.71 -1.7 5,107,685 + BT3933 0.84 -0.5 5,107,694 + BT3933 0.85 -0.9 5,107,694 + BT3933 0.85 +1.1 5,107,756 + -4.8 5,107,757 - +0.1 5,107,798 + -5.0 5,107,802 - -4.6 5,107,810 - -0.5 5,107,813 + -1.9 5,107,814 - -3.2 5,107,844 + -1.1 5,107,844 + +0.1 5,107,935 - -0.8 5,107,943 - -1.5 5,108,037 + -1.7 5,108,037 + -1.9 5,108,037 + -1.0 5,108,037 + -6.3 5,108,038 - -1.7 5,108,110 - -3.8
Or see this region's nucleotide sequence