Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2270 and BT2271 are separated by 61 nucleotides BT2271 and BT2272 are separated by 102 nucleotides BT2272 and BT2273 are separated by 34 nucleotides BT2273 and BT2274 are separated by 6 nucleotides
BT2270: BT2270 - integral membrane protein (MarC family) (NCBI ptt file), at 2,851,712 to 2,852,371
BT2270
BT2271: BT2271 - haloacid dehalogenase-like hydrolase (NCBI ptt file), at 2,852,433 to 2,853,125
BT2271
BT2272: BT2272 - conserved hypothetical protein (NCBI ptt file), at 2,853,228 to 2,854,082
BT2272
BT2273: BT2273 - methyltransferase (NCBI ptt file), at 2,854,117 to 2,854,791
BT2273
BT2274: BT2274 - hypothetical protein (NCBI ptt file), at 2,854,798 to 2,855,523
BT2274
Position (kb)
2853
2854
2855 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2852.243 kb on - strand, within BT2270 at 2852.243 kb on - strand, within BT2270 at 2852.320 kb on + strand at 2852.321 kb on - strand at 2852.343 kb on - strand at 2852.365 kb on + strand at 2852.389 kb on + strand at 2852.398 kb on + strand at 2852.399 kb on - strand at 2852.414 kb on - strand at 2852.420 kb on + strand at 2852.420 kb on + strand at 2852.420 kb on + strand at 2852.421 kb on - strand at 2852.473 kb on + strand at 2852.473 kb on + strand at 2852.473 kb on + strand at 2852.473 kb on + strand at 2852.473 kb on + strand at 2852.474 kb on - strand at 2852.474 kb on - strand at 2852.516 kb on - strand, within BT2271 at 2852.516 kb on - strand, within BT2271 at 2852.754 kb on - strand, within BT2271 at 2852.755 kb on + strand, within BT2271 at 2852.756 kb on - strand, within BT2271 at 2852.756 kb on - strand, within BT2271 at 2852.762 kb on + strand, within BT2271 at 2852.950 kb on + strand, within BT2271 at 2852.951 kb on - strand, within BT2271 at 2852.993 kb on - strand, within BT2271 at 2852.996 kb on - strand, within BT2271 at 2852.996 kb on - strand, within BT2271 at 2853.040 kb on + strand, within BT2271 at 2853.042 kb on + strand, within BT2271 at 2853.043 kb on - strand, within BT2271 at 2853.138 kb on - strand at 2853.139 kb on + strand at 2853.139 kb on + strand at 2853.140 kb on - strand at 2853.141 kb on + strand at 2853.141 kb on + strand at 2853.143 kb on + strand at 2853.143 kb on + strand at 2853.143 kb on + strand at 2853.143 kb on + strand at 2853.144 kb on - strand at 2853.146 kb on + strand at 2853.173 kb on - strand at 2853.218 kb on + strand at 2853.219 kb on - strand at 2853.224 kb on + strand at 2853.225 kb on - strand at 2853.251 kb on - strand at 2853.284 kb on + strand at 2853.374 kb on - strand, within BT2272 at 2853.396 kb on - strand, within BT2272 at 2853.419 kb on - strand, within BT2272 at 2853.549 kb on - strand, within BT2272 at 2853.580 kb on - strand, within BT2272 at 2853.668 kb on + strand, within BT2272 at 2853.736 kb on + strand, within BT2272 at 2853.736 kb on + strand, within BT2272 at 2853.857 kb on + strand, within BT2272 at 2853.962 kb on + strand, within BT2272 at 2853.982 kb on - strand, within BT2272 at 2854.082 kb on + strand at 2854.127 kb on - strand at 2854.868 kb on - strand at 2854.906 kb on - strand, within BT2274 at 2854.970 kb on - strand, within BT2274 at 2855.018 kb on - strand, within BT2274 at 2855.036 kb on - strand, within BT2274 at 2855.072 kb on - strand, within BT2274
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 2,852,243 - BT2270 0.80 +0.1 2,852,243 - BT2270 0.80 -0.9 2,852,320 + -0.5 2,852,321 - -1.3 2,852,343 - -0.8 2,852,365 + -2.1 2,852,389 + -1.5 2,852,398 + -3.3 2,852,399 - +2.1 2,852,414 - -1.2 2,852,420 + -0.3 2,852,420 + -0.7 2,852,420 + -1.9 2,852,421 - -0.9 2,852,473 + +0.1 2,852,473 + -0.5 2,852,473 + -2.2 2,852,473 + -1.4 2,852,473 + -2.3 2,852,474 - +1.7 2,852,474 - +0.1 2,852,516 - BT2271 0.12 -1.7 2,852,516 - BT2271 0.12 -0.9 2,852,754 - BT2271 0.46 -0.5 2,852,755 + BT2271 0.46 +0.7 2,852,756 - BT2271 0.47 +0.1 2,852,756 - BT2271 0.47 +1.1 2,852,762 + BT2271 0.47 -0.9 2,852,950 + BT2271 0.75 -2.2 2,852,951 - BT2271 0.75 -0.9 2,852,993 - BT2271 0.81 -1.4 2,852,996 - BT2271 0.81 -1.7 2,852,996 - BT2271 0.81 -0.5 2,853,040 + BT2271 0.88 -2.3 2,853,042 + BT2271 0.88 +1.3 2,853,043 - BT2271 0.88 -1.8 2,853,138 - +1.1 2,853,139 + +2.1 2,853,139 + -1.5 2,853,140 - -1.6 2,853,141 + -0.3 2,853,141 + -1.7 2,853,143 + +0.1 2,853,143 + -0.1 2,853,143 + -0.5 2,853,143 + -0.3 2,853,144 - -0.7 2,853,146 + -1.9 2,853,173 - +0.6 2,853,218 + +1.7 2,853,219 - -2.4 2,853,224 + -0.3 2,853,225 - -1.2 2,853,251 - -0.5 2,853,284 + -1.7 2,853,374 - BT2272 0.17 -2.0 2,853,396 - BT2272 0.20 -0.5 2,853,419 - BT2272 0.22 -2.3 2,853,549 - BT2272 0.38 -1.7 2,853,580 - BT2272 0.41 -1.3 2,853,668 + BT2272 0.51 -1.3 2,853,736 + BT2272 0.59 +2.1 2,853,736 + BT2272 0.59 -3.6 2,853,857 + BT2272 0.74 +1.1 2,853,962 + BT2272 0.86 -0.5 2,853,982 - BT2272 0.88 +1.1 2,854,082 + -1.5 2,854,127 - -2.1 2,854,868 - +1.1 2,854,906 - BT2274 0.15 -1.9 2,854,970 - BT2274 0.24 -1.0 2,855,018 - BT2274 0.30 -0.4 2,855,036 - BT2274 0.33 -0.2 2,855,072 - BT2274 0.38 -1.7
Or see this region's nucleotide sequence