Experiment: Varel_Bryant medium with Chlorpromazine 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2168 and BT2169 are separated by 19 nucleotides BT2169 and BT2170 are separated by 134 nucleotides BT2170 and BT2171 are separated by 24 nucleotides BT2171 and BT2172 are separated by 5 nucleotides
BT2168: BT2168 - coproporphyrinogen III oxidase (NCBI ptt file), at 2,728,034 to 2,729,164
BT2168
BT2169: BT2169 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,729,184 to 2,729,732
BT2169
BT2170: BT2170 - hypothetical protein (NCBI ptt file), at 2,729,867 to 2,730,301
BT2170
BT2171: BT2171 - putative anti-sigma factor (NCBI ptt file), at 2,730,326 to 2,731,198
BT2171
BT2172: BT2172 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 2,731,204 to 2,733,897
BT2172
Position (kb)
2729
2730
2731 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2728.875 kb on + strand, within BT2168 at 2728.877 kb on + strand, within BT2168 at 2728.890 kb on + strand, within BT2168 at 2728.890 kb on + strand, within BT2168 at 2728.968 kb on + strand, within BT2168 at 2728.969 kb on - strand, within BT2168 at 2728.992 kb on + strand, within BT2168 at 2729.057 kb on + strand at 2729.097 kb on + strand at 2729.137 kb on + strand at 2729.163 kb on - strand at 2729.259 kb on + strand, within BT2169 at 2729.259 kb on + strand, within BT2169 at 2729.260 kb on - strand, within BT2169 at 2729.260 kb on - strand, within BT2169 at 2729.260 kb on - strand, within BT2169 at 2729.261 kb on + strand, within BT2169 at 2729.271 kb on + strand, within BT2169 at 2729.296 kb on - strand, within BT2169 at 2729.301 kb on + strand, within BT2169 at 2729.461 kb on - strand, within BT2169 at 2729.662 kb on + strand, within BT2169 at 2729.683 kb on + strand at 2729.683 kb on + strand at 2729.683 kb on + strand at 2729.770 kb on + strand at 2729.825 kb on + strand at 2729.879 kb on - strand at 2729.910 kb on + strand at 2729.958 kb on - strand, within BT2170 at 2729.963 kb on - strand, within BT2170 at 2729.970 kb on - strand, within BT2170 at 2729.970 kb on - strand, within BT2170 at 2729.970 kb on - strand, within BT2170 at 2730.125 kb on - strand, within BT2170 at 2730.192 kb on + strand, within BT2170 at 2730.192 kb on + strand, within BT2170 at 2730.195 kb on - strand, within BT2170 at 2730.211 kb on - strand, within BT2170 at 2730.235 kb on - strand, within BT2170 at 2730.235 kb on - strand, within BT2170 at 2730.267 kb on + strand at 2730.267 kb on + strand at 2730.268 kb on - strand at 2730.316 kb on - strand at 2730.332 kb on + strand at 2730.357 kb on + strand at 2730.498 kb on + strand, within BT2171 at 2730.531 kb on - strand, within BT2171 at 2730.536 kb on + strand, within BT2171 at 2730.537 kb on - strand, within BT2171 at 2730.537 kb on - strand, within BT2171 at 2730.621 kb on + strand, within BT2171 at 2730.622 kb on - strand, within BT2171 at 2730.630 kb on - strand, within BT2171 at 2730.630 kb on - strand, within BT2171 at 2730.699 kb on - strand, within BT2171 at 2730.709 kb on - strand, within BT2171 at 2730.712 kb on + strand, within BT2171 at 2730.713 kb on - strand, within BT2171 at 2730.734 kb on - strand, within BT2171 at 2730.765 kb on + strand, within BT2171 at 2730.794 kb on + strand, within BT2171 at 2730.825 kb on + strand, within BT2171 at 2730.868 kb on - strand, within BT2171 at 2730.900 kb on + strand, within BT2171 at 2730.902 kb on + strand, within BT2171 at 2730.902 kb on + strand at 2730.902 kb on + strand, within BT2171 at 2730.902 kb on + strand, within BT2171 at 2730.903 kb on - strand, within BT2171 at 2730.903 kb on - strand, within BT2171 at 2730.936 kb on + strand, within BT2171 at 2731.131 kb on + strand at 2731.138 kb on + strand at 2731.180 kb on + strand at 2731.181 kb on - strand at 2731.183 kb on + strand at 2731.201 kb on + strand at 2731.202 kb on - strand at 2731.212 kb on + strand at 2731.254 kb on - strand at 2731.257 kb on + strand at 2731.257 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Chlorpromazine 0.05 mM remove 2,728,875 + BT2168 0.74 -2.0 2,728,877 + BT2168 0.75 -2.2 2,728,890 + BT2168 0.76 -1.8 2,728,890 + BT2168 0.76 -1.3 2,728,968 + BT2168 0.83 -1.4 2,728,969 - BT2168 0.83 -1.8 2,728,992 + BT2168 0.85 +0.1 2,729,057 + -1.5 2,729,097 + +0.4 2,729,137 + -1.9 2,729,163 - -1.3 2,729,259 + BT2169 0.14 -1.8 2,729,259 + BT2169 0.14 +0.6 2,729,260 - BT2169 0.14 -4.2 2,729,260 - BT2169 0.14 -1.9 2,729,260 - BT2169 0.14 -4.7 2,729,261 + BT2169 0.14 -3.3 2,729,271 + BT2169 0.16 +1.6 2,729,296 - BT2169 0.20 -0.9 2,729,301 + BT2169 0.21 -1.3 2,729,461 - BT2169 0.50 -2.5 2,729,662 + BT2169 0.87 +0.1 2,729,683 + -0.9 2,729,683 + -2.7 2,729,683 + -1.7 2,729,770 + +0.1 2,729,825 + -0.3 2,729,879 - -3.7 2,729,910 + -1.0 2,729,958 - BT2170 0.21 -4.2 2,729,963 - BT2170 0.22 -2.6 2,729,970 - BT2170 0.24 -5.3 2,729,970 - BT2170 0.24 -2.7 2,729,970 - BT2170 0.24 -4.1 2,730,125 - BT2170 0.59 +0.0 2,730,192 + BT2170 0.75 -2.3 2,730,192 + BT2170 0.75 -2.2 2,730,195 - BT2170 0.75 -4.2 2,730,211 - BT2170 0.79 -2.9 2,730,235 - BT2170 0.85 -4.0 2,730,235 - BT2170 0.85 -4.0 2,730,267 + -1.8 2,730,267 + -1.9 2,730,268 - -3.4 2,730,316 - -2.3 2,730,332 + -0.8 2,730,357 + -2.0 2,730,498 + BT2171 0.20 -0.7 2,730,531 - BT2171 0.23 -2.3 2,730,536 + BT2171 0.24 +1.1 2,730,537 - BT2171 0.24 -0.5 2,730,537 - BT2171 0.24 -1.3 2,730,621 + BT2171 0.34 +0.5 2,730,622 - BT2171 0.34 -1.3 2,730,630 - BT2171 0.35 -2.6 2,730,630 - BT2171 0.35 +1.1 2,730,699 - BT2171 0.43 -1.1 2,730,709 - BT2171 0.44 -0.3 2,730,712 + BT2171 0.44 -2.9 2,730,713 - BT2171 0.44 -1.3 2,730,734 - BT2171 0.47 -0.9 2,730,765 + BT2171 0.50 -1.3 2,730,794 + BT2171 0.54 -2.8 2,730,825 + BT2171 0.57 -0.8 2,730,868 - BT2171 0.62 -0.8 2,730,900 + BT2171 0.66 +0.7 2,730,902 + BT2171 0.66 -2.3 2,730,902 + +0.5 2,730,902 + BT2171 0.66 -0.4 2,730,902 + BT2171 0.66 +1.1 2,730,903 - BT2171 0.66 -2.9 2,730,903 - BT2171 0.66 +0.1 2,730,936 + BT2171 0.70 -1.5 2,731,131 + -1.5 2,731,138 + -1.2 2,731,180 + -3.5 2,731,181 - -1.5 2,731,183 + -2.5 2,731,201 + -0.9 2,731,202 - -4.1 2,731,212 + -2.1 2,731,254 - -4.4 2,731,257 + -1.1 2,731,257 + -3.8
Or see this region's nucleotide sequence